| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,605,533 | C→T | S161S (AGC→AGT) | lsrB → | Autoinducer‑2 ABC transporter periplasmic binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,605,533 | 0 | C | T | 93.8% | 34.8 / ‑6.2 | 16 | S161S (AGC→AGT) | lsrB | Autoinducer‑2 ABC transporter periplasmic binding protein |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (8/7); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGC > NC_000913/1605294‑1605745 | aatcaaGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGGGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTCCTCAAGTCCCACCGTTan < 1:312671/251‑2 (MQ=255) gctgTTTCGCCTGATGTCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGTGATTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCATGGAACGCCCGCCCAGTTATGAGGTATGTTTGTGGATATGGCGGTGCTTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTGTTTCTACTCAAGTCGCACCGTTACGGACCAAAACCAGTGGGTGAAAGaagc < 1:329218/245‑1 (MQ=255) gCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGCCTCTGATACTAAACCGGAGTGCCGCTATTACTACATTAATCAGGGAACGCCCGACCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAgatcggaagagcacacgtctaactcc > 1:330162/1‑226 (MQ=255) ncGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGc > 1:133604/2‑251 (MQ=255) cAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGCGGATATGGCGGCGCGTCAGGTGAATACAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGtccc > 1:433311/1‑99 (MQ=255) aGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCCAAGTCGAGTTTTTCTACTCAAGTCCCACCGTTACg > 1:162910/1‑92 (MQ=255) ntATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAACGTCGCGTTTTTCTACTCAAGTCCCACCGTTAAGGACCAAAACCAGTGGGTg > 1:62112/2‑101 (MQ=255) gTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCTAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGAt < 1:272276/172‑1 (MQ=255) ggtggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCCAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAAACCGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGAATCCAGGCTGGGAAATTGTCACTTCGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCCGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACg > 1:488673/1‑251 (MQ=255) tggATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAGTggn < 1:93509/90‑2 (MQ=255) atatGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTGTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTg < 1:334524/251‑1 (MQ=255) gTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAATGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGcc < 1:438897/221‑1 (MQ=255) gTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTCCGGACCAAAACCAGTGGGTGAAAGAAGCGCAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTg > 1:291225/1‑234 (MQ=255) gAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGTCCCACCGCTTCGGCCCCAATGCAGTGGGTGAAAGAGGCGACAGAGAACAACGCCAAAGAGAATc > 1:324664/1‑104 (MQ=255) gcagagttgtgctctaccgatcttttttCAACTCAAGCCCCCCCGTTACGGACCAAAACCAGTGGGTAAAAAAAGCGAAAGCGAAAATCGCCAAAGAGCACCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATACCGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCTTATAGCGATCCCGACCCCATTATCGCCCCCGTTCCCAACGCCCTGCCCTCTGCCGCACAAGCCGc < 1:119053/227‑1 (MQ=255) ccAAAGTCGCGTTTTTCTACTCAAGTCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGGGAAAATCTCCAAAGAGCATCCACGCTGGGAAATAGTCACTACGCATTTTGGCTATAACGATGCCACTAAATCGTTACAAAcccc < 1:463864/154‑3 (MQ=255) cAAGTCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCCGTTTGGCTATAACGATGCCACTAAAt > 1:280165/1‑120 (MQ=255) | AATCAAGGTTATAACGCCATTATCGTTTCTGCGGTTTCGCCTGATGGCTTGTGTCCGGCACTGAAACGCGCCATGCAACGTGGTGTGAGAGTGCTGACCTGGGACTCTGATACTAAACCGGAGTGCCGCTCTTACTACATTAATCAGGGAACGCCCGCCCAGTTAGGAGGTATGTTGGTGGATATGGCGGCGCGTCAGGTGAATAAAGACAAAGCCAAAGTCGCGTTTTTCTACTCAAGCCCCACCGTTACGGACCAAAACCAGTGGGTGAAAGAAGCGAAAGCGAAAATCGCCAAAGAGCATCCAGGCTGGGAAATTGTCACTACGCAGTTTGGCTATAACGATGCCACTAAATCGTTACAAACCGCAGAAGGAATATTAAAAGCGTATAGCGATCTCGACGCCATTATCGCCCCCGATGCCAACGCCCTGCCCGCTGCCGCACAAGCCGC > NC_000913/1605294‑1605745 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |