Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	690,948	G→A	V279V (GTC→GTT)	ybeX ←	CorC‑HlyC family protein YbeX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	690,948	0	G	A	100.0%	52.0 / NA	20	V279V (GTC→GTT)	ybeX	CorC‑HlyC family protein YbeX
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/9);  total (11/9)

CCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCT  >  NC_000913/690755‑691188                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 ccATCAGCGAAATAATCGCCGCAGGCCAGACGTAGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCg                                                                                                                                                                                         >  1:410919/1‑251 (MQ=255)                 cgccgcAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGttt                                                                                                                                                                         >  1:159667/1‑251 (MQ=255)                                               ggCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCCGCAGGCGAATGCGCTGGCGTTCCATTAATGAAGCAAAAACCATGTAGTTATCTATACAGTTTCGGTTTTATTCATCCACCTTCGGCTGCGGTGAGTCATCCGGGATTTTACCATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCgtcga                                                                                                                                                                                     >  1:245451/1‑209 (MQ=255)                                                    gCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTTGGGTGAGTCATCCGGGATTTTAACATGAACCGGAATAATTCGCCTACTGTCGGCCATCGCCACTTTGAACTGTTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATc                                                                                                                                      <  1:482158/251‑1 (MQ=255)                                                               gCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAAATAATGTGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGTGTCATCCGGGATTTTAANATGNNNNNGannnnnnnnnn                                                                                                                                                                                                                            >  2:301263/1‑144 (MQ=255)                                                                    gAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTAAATTAATGAGGCAAAAGCCATGTAGTTCTCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGGGCGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGc                                                                                                                                         >  1:80881/1‑232 (MQ=255)                                                                                        ggCGACTGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATATATCGAGTTTCGGTTTTATTAATTAAGCTTCGGCTGGGGTGAGTAATACGGGATTTTACCATGGACCTGAATAATACGCCAAATGTCGGCCATCGCCAATTTGAACTGGTATCCGTAGATGTCGCTAGTTTCGCCACACGCCGGGAGATGCACAAAt                                                                                                                                               >  1:294215/1‑206 (MQ=255)                                                                                                          naaTTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATACAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCa                                                                                                                                                                    >  1:148527/2‑167 (MQ=255)                                                                                                           aaTTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTGTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGGCGGCCATCGCCACGTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGGCCACCGATAGTGTCGACTTCTTCATCGCTAAAGGGGGTGCCGAACGCtt                                                                               <  1:209828/251‑1 (MQ=255)                                                                                                                       aaaaGTCATTTAGTTATCTATCCAATTTCGGTGTTATTCATCCAGCTTCTGCTTGTGTGATTCTGCCGTGATTTTAACATTAACCTGAATAGTACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAAcc                                                                                                                                                                                          <  1:345591/132‑1 (MQ=255)                                                                                                                       aaaaGCCCNGNNNTNNTCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGANTCATCCGGGATTTTANCANGAACNTGANTAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAAcc                                                                                                                                                                                          >  2:345591/1‑132 (MQ=255)                                                                                                                             cATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGTATTTTAACATGAACCTGAATAATCCGCCGACTGTCGGCCATCGCCGCTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGc                                                                                                                                         <  1:454883/175‑1 (MQ=255)                                                                                                                                  aGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCGGGGTTGAGTCAGCCTGGATTTTAACATGAACCTGAATAAGACGCCGACTGTCgn                                                                                                                                                                                                                   <  1:101420/96‑2 (MQ=255)                                                                                                                                   gTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCGAAAGGGGGGGCCGAACGCTTCGTGGAAGTc                                                                     <  1:399092/237‑1 (MQ=255)                                                                                                                                          atcCAGTTTCGGTTTTATTCATCCAGCATCGGCTGGGGTGAGTCATCCGGGATTGTAACATGAGCCTGAATAATACGCGGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGGCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCAGCAGACCACCGATAGTGGCGACGTCTGCATCGCTAAAGCGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTg                                                    <  1:67236/247‑1 (MQ=255)                                                                                                                                                             cATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTANCATNAACCNGANTAATACGCCGACTTTNGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTGNNCNNNGNGCCGGAAg                                                                                                                                                         <  2:356717/127‑1 (MQ=255)                                                                                                                                                             cATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTAGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAg                                                                                                                                                         >  1:356717/1‑127 (MQ=255)                                                                                                                                                                         ncTGGGGTGAGTCATCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAgtgt                                                                                                                   >  1:97899/2‑153 (MQ=255)                                                                                                                                                                                     naTCCGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTg                                                                                         >  1:320243/2‑167 (MQ=255)                                                                                                                                                                                        ccGGGATTTTAACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATACCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTGGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCAtct  <  1:502951/251‑1 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                                                 CCATCAGCGAAATAATCGCCGCAGGCCAGACGTCGTAAGGAGAGAAGGCCAGCGTTCCGCAGGCACCGAATAATAACGCCAGCAGCAGGCGAATGCGCTGGCGTTCAATTAATGAGGCAAAAGCCATGTAGTTATCTATCCAGTTTCGGTTTTATTCATCCAGCTTCGGCTGGGGTGAGTCATCCGGGATTTTGACATGAACCTGAATAATACGCCGACTGTCGGCCATCGCCACTTTGAACTGGTAACCGTCGATGTCGATAGTTTCGCCACGCGCCGGAAGATGCCCAAATGCCTGCATCACCAGACCACCGATAGTGTCGACTTCTTCATCGCTAAAGTGGGTGCCGAACGCTTCGTTGAAGTCTTCAATGGAAGCCAGTGCGCGTACGGTCCAGGTATGACGACTCAGCTGACGGAAGTCGATATCATCT  >  NC_000913/690755‑691188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑