Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,340,381 G→A V47I (GTA→ATA)  lapA → lipopolysaccharide assembly protein A

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,340,3810GA96.2% 69.7 / ‑6.1 26V47I (GTA→ATA) lapAlipopolysaccharide assembly protein A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/14);  minor base C (0/1);  total (11/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GTGTGGATGCCATTTCGCAGACGGCGCGAAAATGGTACTTTAAAGGGCTATTGCGGTAAGTTGACCATAATTTATTCGCTCTAACCACATAACGGGAAGTAATGTGAAATATTTACTCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGGTATTGTTTGCTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGCCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGG  >  NC_000913/1340140‑1340608
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   
gtgtGGATGTCATTTAGCAGACGGCGCGCAAATGGTACTTTAAAGGGCTATTGCGGTAAGTTGACCATAATTTATTCGCTCTAACCACATAACGGGAAGTAATGTGAAATATTTACTCATTTTATTACTGGTGTTAGCGATCTTCGTGCTTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGttt                                                                                                                                                                                                                             >  1:153402/1‑250 (MQ=255)
        gCCATTTCGCAGACGGCGCGAAAATGGTACTTTAAAGGGCTATTGCGGTAAGTTGACCATAATTTATTCGCTCTAACCACATAACGGGAAGTAATGTGAAATATTTACTCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCgggg                                                                                                                                                                                                                    <  1:81206/251‑1 (MQ=255)
          cATTTCGCAGACGGCGCGAAAATGGTACTTTAAAGGGCTATTGCGGTAAGTTGACAATACTTTATTCGCTCTAACAACATAACGGGAAGTAATGTGAAATCTTTAATCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGATCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACCTTGCTGGCGATATTGTTTACTGCGGGGtt                                                                                                                                                                                                                  >  1:276352/1‑251 (MQ=255)
                                 ggttcTTTTAAGGGCTATTGCCGTAAGTTGCCCATAATTTTTTCTGTCTCACCATATAACGTGCAGTAATGTGATAGATTTTCGCTTTTTCTCACTGGTGTTAGCGATTTCCGGTATTTCGTTCACCTTGGGTACGCAGAACCATCCACTTGGGGCGTTTAATTATATGCTAGCGCTATGGGAGTCCCGTATCGGCAGAGTGGTGGGGCTTTTGTTTATTGCGGGGTTTGCTATCGGTTGGTTGATTtgtg                                                                                                                                                                                           <  1:456991/247‑1 (MQ=255)
                                                      ggTAAGTTGACCATAATTTATTCGCTCTAACCACATAACGGGAAGTAATGTGAAATATTTACTCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTgg                                                                                                                                                                                >  1:134610/1‑241 (MQ=255)
                                                                         aTTCGCTCTAACCACATAACGGGAAGTAATGTGAAATATTTACTCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTGAATTATCTGTTAGCGCAAGTGGAGTACCGTATTGCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTtgtg                                                                                                                                                                                           <  1:295630/211‑1 (MQ=255)
                                                                                                       gtgAAATATTTACTCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAAcc                                                                                                                      >  1:120531/1‑250 (MQ=255)
                                                                                                                                      tAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTCTCTGTTAGCGCAAGGGGAGTACCGTCTTTCCACATTGCTGGCGATATTGTTTAATGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGCGTTCGTGTTTCCCTGgcgcgc                                                                                                                                                     >  1:208323/1‑188 (MQ=255)
                                                                                                                                             cTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTGAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCTGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGgcgcgc                                                                                                                                                     <  1:376338/181‑1 (MQ=255)
                                                                                                                                             cTTCGNNCTNNNGGNNACGTTGGGTGCGCAGAACGATCANCAGGTGACGTTTAATTATCTGTTAGCGCANGNGNAGTANNGTANTTCCACATTGNTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGNCNTGTTTTGGCTGCGAGTTCGTGTTTCCCTGgcncgc                                                                                                                                                     >  2:376338/1‑181 (MQ=255)
                                                                                                                                               tCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGc                                                                                                                                                                                                               <  1:288628/121‑1 (MQ=255)
                                                                                                                                               tCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGGTTGCTATCGGTTGGTTGATTTGTGGTCTGTTCTGGCTGCGATTTCGTGTTTCCCTGGCGCGCGCTTAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGCCGCan                                                                                <  1:103442/248‑2 (MQ=255)
                                                                                                                                                  tGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTgn                                                                                                                                                                                                               <  1:132997/118‑2 (MQ=255)
                                                                                                                                                  tGATTTCGGGCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTATCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTtgtg                                                                                                                                                                                           <  1:342331/138‑1 (MQ=255)
                                                                                                                                                         ggTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCACGGGGAGTACCGCATTTCCCCATTGCTGGAGATATTGTTTACTGCGGGGTTTGCTATAGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGa                                                                                                                                                                          >  1:252581/1‑148 (MQ=255)
                                                                                                                                                          ntCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGt                                                                                                                                                                                                                               >  1:311796/2‑94 (MQ=255)
                                                                                                                                                          gtcgcGTTGGGTGCGCAGAACGATCAACAGGGGACGTTTAATTAGCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATGGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGATGTCGTGTTTCTCTGGCGCGCGCGGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCTCCCGCGACTGACGTGGCTGTAGTGCCGCAc                                                                                <  1:473251/233‑1 (MQ=255)
                                                                                                                                                          gTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGt                                                                                                                                                                                                                               <  2:311796/94‑1 (MQ=255)
                                                                                                                                                             aCGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGgcgcgcgc                                                                                                                                                   <  1:86010/167‑1 (MQ=255)
                                                                                                                                                                    gTGCGCAGAACGATCAACAGGTGANGTTTAATTATNTGTNNGCGCNANGNGAGTACCGTATTTCCACATTGCTGGCGATNTTGTTTACTGCGGGGTTTGCTATNNGTNNNTTNNTTtgt                                                                                                                                                                                            <  2:369297/119‑1 (MQ=255)
                                                                                                                                                                    gTGCGCAGAACGATCAACAGGAGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTtgt                                                                                                                                                                                            >  1:369297/1‑119 (MQ=255)
                                                                                                                                                                                                            aGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGAc                                                                                                      >  1:11941/1‑165 (MQ=255)
                                                                                                                                                                                                                        gTACCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCg                                                                                                                                                                           >  1:221936/1‑84 (MQ=255)
                                                                                                                                                                                                                          aCCGTATTTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGCCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTgg  <  1:79521/251‑1 (MQ=255)
                                                                                                                                                                                                                          aCCGTATGTCCACATTGCTGGCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTGTCACCCGCTACTGACGTGGCTGTAGTGCCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTgg  <  1:79504/251‑1 (MQ=255)
                                                                                                                                                                                                                                             ggCGATATTGTTTACTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTTGCTGCGAGTTCGTGTGTCCCTGTCGCGCGCTGAACTTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGc                                                                                             <  1:407921/141‑1 (MQ=255)
                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                   
GTGTGGATGCCATTTCGCAGACGGCGCGAAAATGGTACTTTAAAGGGCTATTGCGGTAAGTTGACCATAATTTATTCGCTCTAACCACATAACGGGAAGTAATGTGAAATATTTACTCATTTTCTTACTGGTGTTAGCGATCTTCGTGATTTCGGTCACGTTGGGTGCGCAGAACGATCAACAGGTGACGTTTAATTATCTGTTAGCGCAAGGGGAGTACCGTATTTCCACATTGCTGGCGGTATTGTTTGCTGCGGGGTTTGCTATCGGTTGGTTGATTTGTGGCCTGTTCTGGCTGCGAGTTCGTGTTTCCCTGGCGCGCGCTGAACGTAAAATAAAGCGACTGGAAAACCAGCTTTCACCCGCGACTGACGTGGCTGTAGTGCCGCACTCGTCAGCGGCGAAGGAATAACTTTCTATGCTGGAGTTGTTGTTTCTGCTTTTGCCTGTAGCCGCTGCCTATGGCTGG  >  NC_000913/1340140‑1340608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑