| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 374,464 | C→T | H199H (CAC→CAT) | mhpE → | 4‑hydroxy‑2‑oxovalerate aldolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 374,464 | 0 | C | T | 95.0% | 47.8 / ‑5.9 | 20 | H199H (CAC→CAT) | mhpE | 4‑hydroxy‑2‑oxovalerate aldolase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/11); minor base G (0/1); total (8/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
CCCGCGAGCTCGGAATGGACACCGTTGGTTTTCTGATGATGAGCCATATGACCACGCCGGAGAATCTCGCCAAGCAGGCAAAGCTGATGGAAGGCTACGGTGCGACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCACCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAG > NC_000913/374244‑374690 | cccGCGAGCTCGGAATGGACACCGTTGGTTTTCTGATGATGAGCCATATGACCACGCCGGAGAATCTCGCCAAGCAGGCAAAGCTGATGGAAGGCTACGGTGCGACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAActct > 1:177549/1‑251 (MQ=255) acCGTTGGTGTTCTGATGATGAGCCATATGACCACGCCGGAGAAGCTCGCAAAGCAGGCAAAGCTGATGGAAGGCTACGGTGCGACCGGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATAGCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGATTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAggn < 1:46917/251‑2 (MQ=255) tGACCACGCCGGAGAATCTCGCCAAGCAGGCAAAGCTGATGCAAGGCTAAGGTACCAAATGCCATCATGAAGGGCATGACGTCGGAGCGATGAAAATGAGCGATATTCGAGACACTTTCCGAGACCTGAAAGCAGAGCTGAAAACAGAACAGCAAACTGCCATGCAACCTAATCTTAACCTGAGTCTTGGAGTGGCGAACTCTATCGCGGAGGAGGAAGTGGGCTGTGACCGAATCGACGCACGCCTcgcg > 1:376513/1‑251 (MQ=255) aCGCCGGAGAATCTCGCCAAGCAGGCAAAGCGGATGGAAGGCTACGGTGCGACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCtn < 1:149093/221‑2 (MQ=255) aCGGTGCGACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGTAGGTAACGCACCGCTGGAAGTGTTTATTgcc < 1:418871/251‑1 (MQ=255) aCGGTGCGACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTgcc < 1:418864/251‑1 (MQ=255) gCGACCTGTATTTATGTGGTGGATTCGGGCGGTGCGATAAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTAAAAGCAGAGCTTAAACCAGAAACGCAAACTGGCATGCACGCTCAGCGTAACCTGATTCTTGGCGTGGCGATCTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACg < 1:503908/187‑1 (MQ=255) gtggtgGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTg < 1:399713/251‑1 (MQ=255) ggtggATTCTGGAGGTGCGATGAACATGAGCGATATCCGTGATCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGGGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGgc > 1:112298/1‑251 (MQ=255) gtggATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTtg < 1:10590/118‑1 (MQ=255) gATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATctct > 1:501092/1‑251 (MQ=255) gTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTgg < 1:359419/212‑1 (MQ=255) ccGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGNAGANGGCGNCGNCCGAATCGACGCCAGNCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTNNTNNNTNCCGCcgcg < 2:345212/193‑1 (MQ=255) ccGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACCCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGGTGCGACCGAATCGACGCCAACCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACAGCTGGAAGTGTTTATTGCCGCcgcg > 1:345212/1‑193 (MQ=255) ccGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCCCGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCGGCGACCGAAGCGACGCCAGCCTCGCGGGAATGGGCGCGGGTGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCGGCGGATAAACTGGGCGGTCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTgg < 1:328085/251‑1 (MQ=255) cccTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGCGGCAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGCCGACCTGGTGCGt > 1:452759/1‑251 (MQ=255) cTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCATCCTAACCTGAGTCTTGGCGTGGCGAACTCTCTcgc > 1:244383/1‑83 (MQ=255) gcnaaanCNNGNNGCGNAAACGGGCATGCACGCACATCNNAACCTGAGTNTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACNCCAGCCTCGNNGGNATGGTNGNNNGNNNAGNTAACGCACCGCTGGAAGTGNTTNNTGCCGCNGCGGATAAACTGGGCTTTCAGCATgg < 2:112298/188‑1 (MQ=255) aaCGCAAACTGGCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGGCGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGTTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAg < 1:218637/251‑1 (MQ=255) gCATGCACGCTCATCATAACCTGAGTCTTGGCGTGGCGCACTCTATCGCGCCGGTGGAAGAGGGCTGCGACCGAATAGACGCCAGCCTCGCGGCAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATctct > 1:466519/1‑180 (MQ=255) | CCCGCGAGCTCGGAATGGACACCGTTGGTTTTCTGATGATGAGCCATATGACCACGCCGGAGAATCTCGCCAAGCAGGCAAAGCTGATGGAAGGCTACGGTGCGACCTGTATTTATGTGGTGGATTCTGGCGGTGCGATGAACATGAGCGATATCCGTGACCGTTTCCGCGCCCTGAAAGCAGAGCTGAAACCAGAAACGCAAACTGGCATGCACGCTCACCATAACCTGAGTCTTGGCGTGGCGAACTCTATCGCGGCGGTGGAAGAGGGCTGCGACCGAATCGACGCCAGCCTCGCGGGAATGGGCGCGGGCGCAGGTAACGCACCGCTGGAAGTGTTTATTGCCGCCGCGGATAAACTGGGCTGGCAGCATGGGACCGATCTCTATGCGTTAATGGATGCCGCCGACGACCTGGTGCGTCCGTTGCAGGATCGACCGGTACGAG > NC_000913/374244‑374690 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |