Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,124,168 C→A G97* (GGA→TGA)  cytR ← DNA‑binding transcriptional repressor CytR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,124,1680CA94.4% 41.5 / ‑5.9 19G97* (GGA→TGA) cytRDNA‑binding transcriptional repressor CytR
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (9/8);  total (10/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01

GGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTT  >  NC_000913/4123972‑4124399
                                                                                                                                                                                                    |                                                                                                                                                                                                                                       
ggCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTAGGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCCTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTc                                                                                                                                                                                                                    >  1:241684/1‑218 (MQ=255)
                               ccATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCGCCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGCGactaat                                                                                                                                                                                                     >  1:321459/1‑202 (MQ=255)
                                     ccATCGGCGGCAGATTACGCTGTTCCTCAAGGCGGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTGGGTGATGATCAAATCTATAAAGGTTGTTTCCTGCTGAGTTGGATGCGCACAGTCGCGAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTc                                                                                                                                                                                                                    <  1:75812/181‑1 (MQ=255)
                                            cggcAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCATATATCATTGATTTg                                                                                                                                                                                                                                 <  1:145020/161‑1 (MQ=255)
                                              ncAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCACTTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCCGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGc                                                                                                                                     >  1:61170/2‑251 (MQ=255)
                                               cAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGAAACAACATGCCATCAATTTGCTTGGAGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGGCGCCAATCAGCACCCGCTATCAGTGATTTGCCGCCGTAACTTCGATATCGCGAATAATTTCGCTAAAGAAGGGAACGCAGATAGCCGGGACAATc                                                                                                                                                                >  1:363441/1‑223 (MQ=255)
                                                   ttACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCAGTGATTTgccg                                                                                                                                                                                                                              <  1:171086/157‑1 (MQ=255)
                                                                 aaTGCTTGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCATAGTCGCCAATCAGCACCAGATATCAGTAATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAATAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTgcgc                                                                                                                         <  1:467820/244‑1 (MQ=255)
                                                                    gCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCt                                                                                                               >  1:11562/1‑251 (MQ=255)
                                                                           tCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTTCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCgg                                                                                                           <  1:78454/248‑1 (MQ=255)
                                                                           tCAAACGGAAGCCTTGAACCCAGCAACAACAGGCCATAAAGTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGAGTTTGATGCGCACAGTCGCCAATCAGCACCAGATATTAGTGATTTGCCGCCGTAACTTCGATACCTCTAATAATTTCGCTAAAGAAGGGATCGCAGATAGCCGGGACAATCAGCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCgg                                                                                                           <  1:78449/248‑1 (MQ=255)
                                                                                            ncccAGCAACAACATGCCGTCAATTTGCTTGGTGCTGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATAAGCACCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGAGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCggg                                                                                       >  1:405309/2‑251 (MQ=255)
                                                                                                             cATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGCTTTTGATCCGCACCGTCGCCAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGAGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTa                                                                                                                                        >  1:271439/1‑185 (MQ=255)
                                                                                                             cATCAAGTTGCTTGGTGAGGATCAAAACGATAAAGGATTTTGCCGGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCAGTGAttt                                                                                                                                                                                                                                  <  1:473613/95‑1 (MQ=255)
                                                                                                                          ggTGATGAGCAAATCGATAAAGTTTTTTTCCTGCTGATTGTGATGCGCACAGCCGCCATGCAGCACCATAGATCAGGGTTGTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGatat                                                                                                                                                                           <  1:237060/137‑1 (MQ=255)
                                                                                                                           gTGATGATCAAATCGAGAAAGGTTTTTTCCTGCTGAGTTTGATGCGCCCAGTCGCCAATCAGCCCCAGATATCTGTGATTTGCCGCCGTAACGTCGAGACCGCGAATAAGTTCGCTAAAGAAGGGATCGCATATATCCGTGACATTCACCAGAATTGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTTCGGTAAATAACCCACTTCCCGGGCCGCTTTTTc                                                                             <  1:254809/230‑1 (MQ=255)
                                                                                                                                   naaaTCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATAAGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTCGCGCCCCATAGGCTGCGGTAAATAACTCACTTCCCGGGCCGCTTTTTCAAcc                                                                         >  1:146503/2‑226 (MQ=255)
                                                                                                                                                                       cgcACAGTCGCCAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTg            >  1:388022/1‑251 (MQ=255)
                                                                                                                                                                                 ccAATCAGCACCAGATATCAGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACttt  >  1:401435/1‑251 (MQ=255)
                                                                                                                                                                                                    |                                                                                                                                                                                                                                       
GGCAGCTCCAGCTCCGGTGCAAATTCGTTCGCCATCACCATCGGCGGCAGATTACGCTGTTCCTCAATGCTGGCATCAAACGGCAGCCTTGAACCCAGCAACAACATGCCATCAATTTGCTTGGTGATGATCAAATCGATAAAGGTTTTTTCCTGCTGATTTTGATGCGCACAGTCGCCAATCAGCACCAGATATCCGTGATTTGCCGCCGTAACTTCGATACCGCGAATAATTTCGCTAAAGAAGGGATCGCAGATATCCGGGACAATCACCAGAATGGTGCGGGATTCATTACGCTTGACGTTGCGCCCCATAGGCTGCGGTAAATAACCCACTTCCCGGGCCGCTTTTTCAACCCGATTACGGGTGGCCTGGGAGACTTTATCGGGATTCATTAATGCTCGGGAGACGGTCGCTGTAGAGACTTT  >  NC_000913/4123972‑4124399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑