Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,986,333	A→G	intergenic (‑205/‑592)	araF ← / → ftnB	arabinose ABC transporter periplasmic binding protein/putative ferritin‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,986,333	0	A	G	96.5%	92.7 / ‑4.3	29	intergenic (‑205/‑592)	araF/ftnB	arabinose ABC transporter periplasmic binding protein/putative ferritin‑like protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (12/16);  total (13/16)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

CCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGACATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTGAGTTTTTGAAATGGTGTAATAACCGCAACTCAAAGATGTGGAAAATGCACGTCATTCATTTCGTCATTAATTATCACTGTGCTCATTAATTAACAGA  >  NC_000913/1986104‑1986576                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    ccAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAACTTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCAAAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGt                                                                                                                                                                                                                                >  1:416336/1‑251 (MQ=255)  cAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTGTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTc                                                                                                                                                                                                                               <  1:193271/251‑1 (MQ=255)                    gtgCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTTACTGTGTTGACATAGTTTGAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCATAATTACAGTGAGAACGGGCAGAAATTGAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGGCGAATGc                                                                                                                                                                                                             <  1:69775/251‑1 (MQ=255)                              ctctcCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAACGACATTAGTTAAATTAATAGGCAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTCATTCTCCATAGGAGCGCACTATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGc                                                                                                                                                                                                                                        >  1:272623/1‑214 (MQ=255)                                            tgtCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAgn                                                                                                                                                                                                                                        <  1:299456/200‑2 (MQ=255)                                                                aaaCGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAgg                                                                                                                                                                                                                                              >  1:170548/1‑174 (MQ=255)                                                                   ngAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTc                                                                                                                                                                                                                   >  1:304426/2‑198 (MQ=255)                                                                                                     gCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGCAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGt                                                                                                                            >  1:256161/1‑251 (MQ=255)                                                                                                             ttttAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACAGCGCAGAATGACAGTGAGAACGTGCATAAATGTAGCGGGAAAAGGCAGAAGGGAAAGCCAATTTGTCAGACAAATTGGCGAATGCACAGCAGATTAATCCAGAAGATTAGCCTGGAAATCCTTGGTGTCTTTgg                                                                                                                                                            <  1:113546/211‑1 (MQ=255)                                                                                                                         gANNNNNGACNGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTc                                                                                                                                                                                                                   <  2:304426/144‑1 (MQ=255)                                                                                                                              gggACTGTGTTGACATAGTTTTAGCGAGAAATTAATTATCCATAGGAGAGCACTATCACATCGCAGAATTACAGTGAGAACGTGCATAACTTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAAACCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTg                                                                                                   >  1:459013/3‑251 (MQ=255)                                                                                                                               tGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTg                                                                                                   <  1:410700/250‑1 (MQ=255)                                                                                                                                gACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTGAg                                                                                                 <  1:251944/251‑1 (MQ=255)                                                                                                                                  cTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTGAGCGGTAAAAGGCATAAGGGAAAGCCAATGTGTCAGACAAATTGTCGAATGCACAGCAGATTAAGCCATAAGAGTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGtcttct                                                                                                                                       <  1:240448/211‑1 (MQ=255)                                                                                                                                                     gCGAGNNATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGc                                                                                                                                                                                                                                        <  2:34428/95‑1 (MQ=255)                                                                                                                                                     gCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGc                                                                                                                                                                                                                                        >  1:34428/1‑95 (MQ=255)                                                                                                                                                                 ttCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATCTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCttgttg                                                                                                                                                                   >  1:435915/1‑152 (MQ=255)                                                                                                                                                                        tAGGAGAGCAATATCACAGCGCAGAAGTACAGTGAGAACTTGCATAAGTTTAGCGGGAAAGGGCATAAGGGAAAGCGATTTTGGCAGACAAATTTGCGAATGCACAGCAGTTTAATCCATAAGATTAGCCTGGa                                                                                                                                                                              <  1:190433/134‑1 (MQ=255)                                                                                                                                                                                           cGCAGAATTACAGTGAGAACGTGCATAAAGTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAAGTGTCGAATGCACAGCAGATTAATCCATACGAGTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCGTTTTAACCAGTCAATATGCCGCAGTGCCTGGCTTTTATTTTTTTAAATTGTGTAATAACCGCAACTCAAAGATGTGGAAAATGCACGTCATTCAttt                                      <  1:487758/251‑1 (MQ=255)                                                                                                                                                                                               gAATTACANNGAGAACGTGNATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTNGAATGCACANNAGNTTAAGNCNNNANNNTANCCTGGAAATCCTTGTTGTCNTTNNTACCCANGCGGGATGTCTTCTTTTTAACCAGTc                                                                                                                           <  2:115478/162‑1 (MQ=255)                                                                                                                                                                                               gAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTTTCGAATGCAcagcag                                                                                                                                                                                                      <  1:403808/87‑1 (MQ=255)                                                                                                                                                                                               gAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTc                                                                                                                           >  1:115478/1‑162 (MQ=255)                                                                                                                                                                                                       gTGAGACCGTGCATAAATTTAGCGGGCAAAGGCATCAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTGAGTTTTTGAAATGGTGTAATAACCGCAACTCAAAGATGTGGAAAATGCACGTCATTCATTTCGTCattaatt                           >  1:136734/1‑250 (MQ=255)                                                                                                                                                                                                       gTGAGAACGTGCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGtctt                                                                                                                                         >  1:167323/1‑140 (MQ=255)                                                                                                                                                                                                                 nnnnnaaTNTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAAGCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAg                                                                                                                             <  2:315405/137‑1 (MQ=255)                                                                                                                                                                                                                 ncATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAg                                                                                                                             >  1:315405/2‑142 (MQ=255)                                                                                                                                                                                                                 gCATAAATTTAGCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGGCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCGATAGGCCGCATGACCTGGCGTGGAGTTTTTGAATTGGTGTAATAACCGCAACTCAAAGATGTGGAGAATGCTCGTCATTCATTTCGTCATTAATTATCACTGTGc                 <  1:328898/250‑1 (MQ=255)                                                                                                                                                                                                                            gCGGGAAAAGGCATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGtc                                                                                                                                           <  1:75662/117‑1 (MQ=255)                                                                                                                                                                                                                               ggAAAAGACATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTGAGTTTTTGAAATGGTGTAATAACCGCAACTCAA‑GATGTGGAAAATGCACGTCATTCATTTCGTCATTAATTATCACTGTGCTCATTAATTAACAGa  >  1:34169/1‑250 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                    CCAGGGCTTTAGTAAATTTGTGCATGGTTCTCTCCAGCTTTAGTGTCGTTTTGTGTAGGGCAAAAACGAATGACATTCGTTAAATTAATCGGAAAACAAAGCATTACCTTTTAACTAAAAGATAAGTGACTGTGTTGACATAGTTTTAGCGAGAAATTAATTCTCCATAGGAGAGCAATATCACATCGCAGAATTACAGTGAGAACGTGCATAAATTTAGCGGGAAAAGACATAAGGGAAAGCCAATTTGTCAGACAAATTGTCGAATGCACAGCAGATTAATCCATAAGATTAGCCTGGAAATCCTTGTTGTCTTTGGTACCCATGCGGGATGTCTTCTTTTTAACCAGTCAATAGGCCGCATTACCTGGCGTTGAGTTTTTGAAATGGTGTAATAACCGCAACTCAAAGATGTGGAAAATGCACGTCATTCATTTCGTCATTAATTATCACTGTGCTCATTAATTAACAGA  >  NC_000913/1986104‑1986576

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑