| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,805,830 | C→T | E41K (GAA→AAA) | waaP ← | lipopolysaccharide core heptose (I) kinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,805,830 | 0 | C | T | 93.3% | 35.2 / NA | 15 | E41K (GAA→AAA) | waaP | lipopolysaccharide core heptose (I) kinase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (7/7); minor base G (0/1); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01 | |||||||||||
GGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGC > NC_000913/3805612‑3805992 | ggTAAGAATAAATGATGCGCGAGTGAATTGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCATGCGATGAATAGCGTTCCTCTCGCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATGGTGTATAATCTCTTGTAATGTCGTCCCCTTGCGCCATTTAAGAAAAAAGCGTTTCCCGGACAGTGTAAAGCGTAATGTACGACGAg < 1:429740/239‑1 (MQ=255) ngAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTc > 1:100259/2‑249 (MQ=255) aTCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGc < 1:79355/250‑1 (MQ=255) cAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAAc < 1:432886/249‑1 (MQ=255) ccACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGCGTAGATTTTTTCTAATCTCTTTTCATGTGGTACCCTTGTGCCATTTAAGAAAATAGCTTTTCCAGGACAGTTTAAAGCGCCATGTACGACGAGTCTCCAGTTCACGAACGACCTCGCCGTTCAGTTTTTTGACCTCTGCAACAGCATCTTTACCACGACAACGTGTGGCAAGCGGCTCTTTTAATTAAACCATCTAAACCCCCTGTAATg > 1:248733/1‑251 (MQ=255) ccACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTGCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACGTCGCCGTTCAGTTTTTGGACCTCTGCAAAAGCATCTTTACCGCGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATg < 1:281208/251‑1 (MQ=255) cACTCTCTGTCTGCGCCCAAAACGGGCTTCCGCAATGAGAGTATATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCTGACAGTTTAAAGCGTAATGTACGACTAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCa < 1:374204/186‑1 (MQ=255) cACTCNNTGNNNGCNNCCAAAACGGGCATCCGCAATGAGNGTAGATTTTTTATAATCTCTTTTAATGTCNTNCNCTTGNNCCANTTAAGAAAATNGCTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCNCNAAAGACCTCGCCGTTCAGTTTTTTGACCTCTNCAAAAGCa > 2:374204/1‑186 (MQ=255) tgtcttCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTGTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTGAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGTCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTAcn < 1:90035/187‑2 (MQ=255) gcgcCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCATTGTGCCCTTTAAGAAAATAGATTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCCCGAAAGACCTCGACGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCAAGCCAAAGTGTGGCAAGCGGCTCTTTTATTTCAACCATCTAAACCACCTGTAATGATATCCGCGGCtt > 1:344331/1‑251 (MQ=255) gCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCACAGTGTGGCAAGCGGCTCTTTTAAtt > 1:447455/1‑201 (MQ=255) cATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTTAAAGCg > 1:418411/1‑93 (MQ=255) gtcgtcCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGc < 1:82800/209‑1 (MQ=255) ccTTGTGCCATTTACGAAAATAGGTTTTCCCGGACAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGACAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCaa > 1:178438/1‑128 (MQ=255) cAGTTTAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTgg < 1:364796/163‑1 (MQ=255) cAGTTNNAANNNTANNGTACGACGAGTCTCCAGTTCACGNAAGACCTCGCCGTTCAGTTTTTTGACCTCNGNANAAGCNNCTTNACCACGCCAANGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGNTNTCCGCGGCTTTTTCTgg > 2:364796/1‑163 (MQ=255) | GGTAATAATAAATGATGCGCGAGTTAATGGATTTAACCCTTTTTCGCCAAACCCAATGCCCTTCATTGTATCAACGCCAACATCACTCAGGCGATGAATAGCGTGCCACTCTCTGTCTGCGCCCAAAACGGGCATCCGCAATGAGAGTAGATTTTTTATAATCTCTTTTAATGTCGTCCCCTTGTGCCATTTAAGAAAATAGCTTTTCCCGGACAGTTCAAAGCGTAATGTACGACGAGTCTCCAGTTCACGAAAGACCTCGCCGTTCAGTTTTTTGACCTCTGCAAAAGCATCTTTACCACGCCAAAGTGTGGCAAGCGGCTCTTTTAATTCAACCATCTAAACCACCTGTAATGATATCCGCGGCTTTTTCTGGCAAGC > NC_000913/3805612‑3805992 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |