Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,358,8590TC94.7% 50.7 / ‑5.6 19Y110C (TAT→TGT) puuPputrescine:H(+) symporter PuuP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (9/9);  minor base A (0/1);  total (9/10)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGC  >  NC_000913/1358615‑1359080
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             
gACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGGAGGTTACCACCCACCCCCACGGCGGCACTTCCGGGAAGAGGGCGGAGGGATAGATTTTCGCCAAAAAGACGTTAATCATCGGCAAAAAGAAACAATcc                                                                                                                                                                                                                          >  1:382621/1‑250 (MQ=255)
                                     aCTACCAGGAAGATAAACACCCCCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACCCCCACGGCGGCACTTCCTGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCgn                                                                                                                                                                                    <  1:62496/251‑2 (MQ=255)
                                                                                                                       tctTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGc                                                                                                                                                      <  1:69643/199‑1 (MQ=255)
                                                                                                                                     gcggTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTTATAGCGGTAAACAGCACACCcg                                                                                     <  1:429146/250‑1 (MQ=255)
                                                                                                                                       ggTTAAAATGGCGACGAAGGATACCACCCACAACCATGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGAc                                                                                                                                                                          >  1:417350/1‑163 (MQ=255)
                                                                                                                                        gTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCCACAAGACGTTAATCATCGGCAAAAAGAGACAATTCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTgg                                                                                                                                >  1:475271/1‑204 (MQ=255)
                                                                                                                                        gTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTGTGCCGTAGCTGATAGc                                                                                                      <  1:410197/230‑1 (MQ=255)
                                                                                                                                                              ccacccacACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCTGGTTAATCGTCTTTTGCTCGTAg                                                                                                                                                              <  1:391543/152‑1 (MQ=255)
                                                                                                                                                                   cacacccacGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCg                                                                                                                                        >  1:275737/1‑169 (MQ=255)
                                                                                                                                                                                   cTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTATGCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGc                                              <  1:336522/243‑1 (MQ=255)
                                                                                                                                                                                      ccGGGAAGAGGGCGGAGAGATAGATTTTCACCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGTCCATAAATCCGACGTGCGGGTTAATCGCCTTTTGCTCGTAGGTATATGCCTAACCGGCCTCCGGAAACTGGCTAACCAGTTTGACGTAGCTGATAGCGGTAAAc                                                                                               <  1:157154/191‑1 (MQ=255)
                                                                                                                                                                                                ggcggcGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGAGATGACCAGCCGACCATAAATCCGNNGAGCGGNnt                                                                                                                                                                                 >  2:32345/1‑99 (MQ=255)
                                                                                                                                                                                                ggCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGACGACCATCCGACCATAAATCCGACGTGCGGGTTAATc                                                                                                                                                                             <  1:32345/103‑1 (MQ=255)
                                                                                                                                                                                                                   gCCAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATACATCCGACGTGCGGGTTAATCg                                                                                                                                                                            >  1:266563/1‑85 (MQ=255)
                                                                                                                                                                                                                    ccAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGATAATCGACTTTTGCGCGTAGGTATAgg                                                                                                                                                       >  1:166869/1‑105 (MQ=255)
                                                                                                                                                                                                                    ccAACAAGACGTTAATCATCGGCAAAAAGAGACAATCCAGCAGCGATGACAAGCCGACCATAAATCCGACGTGTGGGTTAATCGACTTTTGCGAGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACAAGTTTGCCGTAGATGATAGCGGTAAAAAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGAACGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATCCGGACATc     >  1:381832/1‑251 (MQ=255)
                                                                                                                                                                                                                       aaaaGACGTTAATCATCGGCAAAAAGAGAAAATCCAGCAGCGATGACCAGCCGACAATAAACCCGACGGGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCCCCGGAAACTGGGGAACCAGTTTGACGTAGCTGATAGCTGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGc  <  1:168269/249‑1 (MQ=255)
                                                                                                                                                                                                                                          cNAAANGAGANAANCCAGCAGCGATGACCNGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTNNGNNNANGCCGAAcc                                                                                                                                                <  2:349019/90‑1 (MQ=255)
                                                                                                                                                                                                                                          cAAAAAGAGACAATCCAGCAGCGATGACCATTCGACCATAAACCCGACGTGCGGGGTATGCGTCTTTTGCGCGTAGGTATAGTCCGAACCTTCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGc                                                                                                      <  1:471276/132‑1 (MQ=255)
                                                                                                                                                                                                                                          cAAAAAGAGACAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGCCTTTTGCGCGTAGGTATAGGCCGCAcc                                                                                                                                                >  1:349019/1‑90 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                             
GACGGTGCCAACGCCTTCTCCTTTATGCAGTCCCTGAACTACCAGGAAGATAAACACCACCATGATGGAGATTTGCACCAGTACAAACAGGGTATTGAAGTTAGCGACCAGGTTGACGCTCTTCAGATTCGCGGCGGTTAAAATGGCGACGAAGGTTACCACCCACACCCACGGCGGCACTTCCGGGAAGAGGGCGGAGAGATAGATTTTCGCCAACAAGACGTTAATCATCGGCAAAAAGAGATAATCCAGCAGCGATGACCAGCCGACCATAAATCCGACGTGCGGGTTAATCGACTTTTGCGCGTAGGTATAGGCCGAACCGGCCTCCGGAAACTGGCGAACCAGTTTGCCGTAGCTGATAGCGGTAAACAGCACACCCGCCAGCGCCAGCAAATAGGACGCCGGAACGTGACCGTCGCTAATGCCGGAGACAATACCAAAAGTATCAAATACGGTCATCGGC  >  NC_000913/1358615‑1359080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑