Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,612,957	G→A	D183N (GAT→AAT)	yjjU →	putative patatin‑like phospholipase YjjU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,612,957	0	G	A	100.0%	25.1 / NA	11	D183N (GAT→AAT)	yjjU	putative patatin‑like phospholipase YjjU
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/7);  total (4/7)

CAAGCCAGATGCCGTTGCAAATGGATACCGCCGCGCGGTTGTTTGACAGCGGCAAATCGTTTTATATGTGCGCCTGTCGTCAGGATGACTACGCGCCGAATTACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTACCTGGATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCAGGGCGCTAAAACGTTGGTCGTCATTCGCACTGTGCC‑GTCACAAATGTACTACACGCCGCAGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATC  >  NC_000913/4612751‑4613122                                                                                                                                                                                                               |                                                                                                                                                                       cAAGCCAGATGCCGTTGCAAATGGATACCGCCGCGCGGTTGTTTGACAGCGGCAAATCGTTTTATATGTGCGCCTGTCGTCAGGATGACTACGCGCCGAATTACTGTTTACCAACCAAACAATACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCAGTAACTACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAgg                                                                                                                            <  1:290929/251‑1 (MQ=255)                            ccgccgCGCGGTTGTTTGACAGCGGCAAATCGTTTTATATGTGCGCCTGTCGTCAGGATGACTACGCGCCGAATTACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCGGGGCGCTAAAACGGTgggtaaatcg                                                                                                 >  1:37169/1‑243 (MQ=255)                                                                 tatGTGCGCCTGTCGTCAGGATGACTACGCGCCGAATTACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTGTTATCGTAGCGTAGTGTCGCTGGAAGGCATTAACTACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCATGGCGCTAAAACGTTGgtcgt                                                                                                     <  1:414023/210‑1 (MQ=255)                                                                 tatGTGCGCCTGTCGTCAGGATGACTACGCGACGAATTACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTAACTGAATGGCGGGATCAGTGATGCGATTACGGTTAAAGAGGCGGCAAGGCAGGGCGCAAAAACGTTGGTCGTCATTCGCACTGTGCC‑GTCACAAATGTACTACACGCCGCAGt                                                           >  1:197894/1‑251 (MQ=255)                                                                                 cAGGATGACTACGCGCCGAATTACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCAAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTACCTGAATGGCGGGATCAGTGATGCGACTCCGGTTAAAGAGGCGGCa                                                                                                                               >  1:500496/1‑168 (MQ=255)                                                                                               gcCGAATTACTTGTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTTCAGTGCGATACCTGGCTTTTATCGTAGTGGAGTGTCGCTGGAAGGCATTAACTTCCTGAATGGCGGGATCAGTGAGGCGATTCCGGTTATAGAGGCGGCAAGGCAGGGCGCGAAATCGGTGGTCGTCATTCGCACTGTGCCCATC‑CAAATGTACTACACGCCGCAGTGGTTCAAACGCAGGGAACGCTGGCTGGGTg                             <  1:377358/251‑1 (MQ=255)                                                                                                   aaTTACTTTTTACCAACCAAACAAAACGGGCGGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGTCAAGGCAGGGCGCTAAAACGTTGGTCGTCATTCTCACTGTGCC‑GTCACAAATGTACTACACGCCGCAGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACan                         <  1:94975/251‑2 (MQ=255)                                                                                                     ttACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACGACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCAGGGCGCTAAAACGTTGGTCGTCATTCg                                                                                               <  1:326086/180‑1 (MQ=255)                                                                                                          ttttACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCAggg                                                                                                                       <  1:450834/151‑1 (MQ=255)                                                                                                                          aaaCTGGCTGGATGTGATTCGCGCCTCCAGTGCGCTACAAGGCTTTTATCGTAGCGGAGTGTAGCTGGACCGCATTAAATAACTGAATGGCGGGATCACTGATGCAATTCCGGTTCAAGAGGGGGCACGACAGGGCGCTAAAACGTTGGTCGTCATTCGCACCGTGCC‑GTCACAAATGAACTACACGCCGCCGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATc  >  1:193858/1‑251 (MQ=255)                                                                                                                                                                              aNNGGAGTGNNGCTGGAAGGCATTAACTACCTGAATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCAGGGCGCTAAAACGTTGgtc                                                                                                       <  2:37169/99‑1 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                       CAAGCCAGATGCCGTTGCAAATGGATACCGCCGCGCGGTTGTTTGACAGCGGCAAATCGTTTTATATGTGCGCCTGTCGTCAGGATGACTACGCGCCGAATTACTTTTTACCAACCAAACAAAACTGGCTGGATGTGATTCGCGCCTCCAGTGCGATACCTGGCTTTTATCGTAGCGGAGTGTCGCTGGAAGGCATTAACTACCTGGATGGCGGGATCAGTGATGCGATTCCGGTTAAAGAGGCGGCAAGGCAGGGCGCTAAAACGTTGGTCGTCATTCGCACTGTGCC‑GTCACAAATGTACTACACGCCGCAGTGGTTCAAACGCATGGAACGCTGGCTGGGTGACAGTAGCCTGCAGCCGCTGGTCAATC  >  NC_000913/4612751‑4613122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑