Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,894,839	T→C	L12P (CTC→CCC)	pabB →	aminodeoxychorismate synthase subunit 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,894,839	0	T	C	100.0%	62.5 / NA	20	L12P (CTC→CCC)	pabB	aminodeoxychorismate synthase subunit 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/11);  total (9/11)

CAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGC  >  NC_000913/1894609‑1895057                                                                                                                                                                                                                                       |                                                                                                                                                                                                                           cAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTg                                                                                                                                                                                                        >  1:36107/1‑251 (MQ=255)   gCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCATGGATCCGCTCGACAGCTTTTTGCTGCTGTTCCTGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGa                                                                                                                                                                                                       >  1:68068/1‑250 (MQ=255)                           ncTGGCCGCTGCTCATCACTTGTGCCCTCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTcc                                                                                                                                                                                          >  1:404039/2‑239 (MQ=255)                                                                   ccGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGGAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCATTAGAGTCAGGACTGATGAAGACGGTATCTCCCGCTGTGATTACTTTACCCTGGCGGCAGGACGCCGCTGAAGTTTATTTCTCCCGCGTAAGCCATCTGTCGGGGGCGATACGTGTACACTCCGGCGATGCCGatc                                                                                                                                      <  1:167453/251‑1 (MQ=255)                                                                    ngCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGAAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGatc                                                                                                                                      >  1:104716/2‑250 (MQ=255)                                                                                 tttGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCg                                                                                                                         <  1:8309/250‑1 (MQ=255)                                                                                                        gaAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCATGACTCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgccgagc                                                                                                  <  1:356870/250‑1 (MQ=255)                                                                                                                 ccTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCan                                                                                        <  1:55126/251‑2 (MQ=255)                                                                                                                         gtgctcttcctatcgCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGTCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTTTCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCGGCCGTGGGGGATGCTTTTACGCTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAAcc                                                                                 <  1:83708/236‑1 (MQ=255)                                                                                                                                      tGCTANNTTNNNGCNNCTAATTATTTCATGCTACCCGGCNCATAGCCAGTAGAGTCAGGACTGATGACGNCNTNATCTNNCGCNGTGATTACTTNACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCANCNGCCGTGGGCGATGCTTTTac                                                                                                                                                        >  2:378305/1‑166 (MQ=255)                                                                                                                                      tGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTATAGTCAGGACTGATGAAGACTTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCATGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGTGCGATGCTTTTac                                                                                                                                                        <  1:378305/166‑1 (MQ=255)                                                                                                                                                             tttCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAgn                                                                                                                                                                                                                 <  1:146713/86‑2 (MQ=255)                                                                                                                                                                  tGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGACGACGTTATCTCCCGCTGTGCTTACTTTAACCTGGCGTCAGGACGCCGATGAATTTTATTTCTCCCGCTTAAGCAACCTGCCGTGGGCGTTGCTTTTACACTCCGGCTCCGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCgcc                                                                                                      >  1:492298/1‑188 (MQ=255)                                                                                                                                                                                    cAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCGGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTGTGATATTGTGGTCGCCGAGCCGATTTGCACTGTAACCACTTGCTGTAAAGAAACCGTTGGTAGTGAAAGCGAAAAACGCACAACGACCACTGATGAc                     <  1:215472/251‑1 (MQ=255)                                                                                                                                                                                                 aCTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTcc                                                                                                                                                                                          <  1:217870/73‑1 (MQ=255)                                                                                                                                                                                                 aCTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTGTTACACTCCGGCTATGCCGATCATCCGTAGAGCCGCTTTGATATGGTGGTCGCCGAGCCGATTTGCACTTTAACCACGTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGc        <  1:83794/251‑1 (MQ=255)                                                                                                                                                                                                 aCTGANGAAGANGNNATCTCCCGCTGTGATTACTNTACCCTGGCGTCAGGNCGCCNCTGAAntan                                                                                                                                                                                                  >  2:217870/1‑63 (MQ=255)                                                                                                                                                                                                       gaAGACGTTATCTCCCGCTGTGATTACTTTACCCTGGCGTCAGGACGCCCCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTCCACTCCGGCTCTGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCcagc  >  1:214039/1‑251 (MQ=255)                                                                                                                                                                                                                            naTTACTTTACCATGGCGTCAGGACGCCGCTGAATTTTATGTCTCCCGCTTAAGCCACCTGCCGTTGGCGATGCTTTTac                                                                                                                                                        >  1:308503/2‑80 (MQ=255)                                                                                                                                                                                                                            gATTACTTTACCCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTac                                                                                                                                                        <  2:308503/80‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                           CAGCTCTTCTGCCACCAGCGCGATTGCCTGGCCGCTGCTCATCCCTTGTGCCATCAGTTCCTGGATCCGCTCGACAGCTTTTTGCTGCTGTTCATGGGTGAGTGAAGGTAAACCTGCAAACATTGTTAACTCCTGCTAAATTGTTGGCGCTAATTATTTCATGCTACCCGGCACATAGCCAGTAGAGTCAGGACTGATGAAGACGTTATCTCCCGCTGTGATTACTTTACTCTGGCGTCAGGACGCCGCTGAATTTTATTTCTCCCGCTTAAGCCACCTGCCGTGGGCGATGCTTTTACACTCCGGCTATGCCGATCATCCGTATAGCCGCTTTGATATTGTGGTCGCCGAGCCGATTTGCACTTTAACCACTTTCGGTAAAGAAACCGTTGTTAGTGAAAGCGAAAAACGCACAACGACCACTGATGACCCGCTACAGGTGCTCCAGC  >  NC_000913/1894609‑1895057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑