Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,771,979	G→T	intergenic (‑235/‑302)	yibI ← / → mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,771,979	0	G	T	100.0%	63.4 / NA	22	intergenic (‑235/‑302)	yibI/mtlA	DUF3302 domain‑containing protein YibI/mannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/8);  total (14/8)

TGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTA  >  NC_000913/3771748‑3772162                                                                                                                                                                                                                                        |                                                                                                                                                                                        tGCCTCGGATTCAAGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGGGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTAAACAAACCATcaaa                                                                                                                                                                       >  1:389135/1‑250 (MQ=255)          ttCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAAGGAGTCAGGATGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCCAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTAAATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAAcc                                                                                                                                                                             >  1:457962/1‑235 (MQ=255)                                            gcAGAAGGGAGTCAGGCTGATATTTTGACCATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAACTGCCATTTTTAGCg                                                                                                                            >  1:165351/2‑250 (MQ=255)                                                                                       gcgATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATACCGATTGTATGAAGAAGGCATAACATGCTGTAGATCACATCAGGAGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGc                                                                                                         >  1:209572/1‑226 (MQ=255)                                                                                        cgATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTTATGCAGATCACAAATATTCAACAAACCAGCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATcgc                                                                                                                    <  1:278415/214‑1 (MQ=255)                                                                                             ctcGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATc                                                                                                                      >  1:482503/1‑207 (MQ=255)                                                                                                    tAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTaca                                                                                                >  1:433183/1‑222 (MQ=255)                                                                                                              gAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTAAGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGAGCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGc                                                                                                                             <  1:488835/183‑1 (MQ=255)                                                                                                                                     ttGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAAGCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTAc                                                       <  1:286597/230‑1 (MQ=255)                                                                                                                                                cATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGtgatga                                                                               >  1:36545/1‑195 (MQ=255)                                                                                                                                                                cGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAACACAAAAAtgtg                                                                                                                                                            >  1:193590/1‑102 (MQ=255)                                                                                                                                                                    ggCATACCATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAANTCGNNNNNttnnnnnn                                                                                                        >  2:297299/1‑144 (MQ=255)                                                                                                                                                                    ggCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAACCCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAAGCGCCGCCTTGTTGCTTTTTGACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAggn                   <  1:297299/235‑2 (MQ=255)                                                                                                                                                                    ggCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTac                                                                                                 >  1:227286/1‑157 (MQ=255)                                                                                                                                                                     gCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCAtttt                                                                                                                                 <  1:179325/124‑1 (MQ=255)                                                                                                                                                                     gCATAACATGCTGTAGATCACATCAAGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGGGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTa  <  1:197514/251‑1 (MQ=255)                                                                                                                                                                      cATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGc                                                                                           >  1:450225/1‑161 (MQ=255)                                                                                                                                                                                              ggTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGcc                                                                                                                                      >  1:215646/1‑94 (MQ=255)                                                                                                                                                                                               ntGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACATACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGata                                >  1:315506/2‑195 (MQ=255)                                                                                                                                                                                                   ncNCCNTAAGANAATANCTTGTGATTCAGATCNCAAATATTCAACAAACCAGNNANACAAANAtgtg                                                                                                                                                            <  2:193590/66‑1 (MQ=255)                                                                                                                                                                                                      ccGTAAGAAAATATCTTGTGATTCAGATCACANATATTCAACNNACNATCAANANNNANNNGTNACACTACTCACATTTAAATNCCNNTTTTAGNGAAAATCGCCGCCTTGTTGCtttttt                                                                                                   <  2:114711/121‑1 (MQ=255)                                                                                                                                                                                                      ccGTAAGAAAATATCTTGTGATTCAGATCACAAATATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCtttttt                                                                                                   >  1:114711/1‑121 (MQ=255)                                                                                                                                                                                                                                        |                                                                                                                                                                                        TGCCTCGGATTCACGTTTATCAGTGTTGTTTTTGGGCTGGCAGCCAGAAGGGAGTCAGGCTGATATTTTGACAATAATCCGGGTTCGCGATTCTCGCCATAACACCAAAGAATAATTTTTAGAGGTGATGAGTTGCTTAGTTACATAACGATTGTATGACGAAGGCATAACATGCTGTAGATCACATCAGGTGAACGCCGTAAGAAAATATCTTGTGATTCAGATCACAAAGATTCAACAAACCATCAAAACAAAAATGTGACACTACTCACATTTAAATGCCATTTTTAGCGAAAATCGCCGCCTTGTTGCTTTTTTACACAAGCGTTTTGTGATGAACGTCACGTCAATTACCTCTCTACCCCCTATATTTATGTGATTGATATCACACAAAAGGCCGTCGACTGGACAGTTA  >  NC_000913/3771748‑3772162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑