Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,325,6440CT100.0% 87.9 / NA 30S33N (AGC→AAC) yjdNPF06983 family protein YjdN
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/17);  total (13/17)

GATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGG  >  NC_000913/4325406‑4325843
                                                                                                                                                                                                                                              |                                                                                                                                                                                                       
gATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTATCCTTCTTCGACCCGTTGCGAATCGAGCACCCGCGTAACCCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGACGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAACTTGCATTCCGGACGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTtat                                                                                                                                                                                                >  1:469151/1‑248 (MQ=255)
                                     tttCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATGCCGGAAGGGCAGTTCTCGGGGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAacgtac                                                                                                                                                                        <  1:291151/235‑1 (MQ=255)
                                                     nttGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGtt                                                                                                                                         >  1:54638/2‑250 (MQ=255)
                                                               gAGCACCAGCGTACAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCgg                                                                                                                                    >  1:454373/1‑245 (MQ=255)
                                                                                 ggAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACCTTGGCGTGGGCGATGGCGGAATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGCTTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAgg                                                                                                                                                            >  1:248199/1‑203 (MQ=255)
                                                                                  gAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAAc                                                                                                                                             >  1:453752/1‑217 (MQ=255)
                                                                                       gCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAacgt                                                                                                                                                                          <  1:241378/183‑1 (MQ=255)
                                                                                           gCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCATGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGt                                                                                                                                          <  1:497221/211‑1 (MQ=255)
                                                                                             ttttCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTAGAGAGCAGTTCcgcgn                                                                                                                                                                                 <  1:133240/170‑2 (MQ=255)
                                                                                                                 cGCTCATCATGAGGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTGGATAGTATAGAGCAGTTCCGCGCCCAACGTACGTGGATAATAGGCAAGCGCGGCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAg                                                                            <  1:275865/251‑1 (MQ=255)
                                                                                                                     catcatGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACgg                                                                                                           <  1:37092/216‑1 (MQ=255)
                                                                                                                      atcatGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAgg                                                                                                                         <  1:288542/201‑1 (MQ=255)
                                                                                                                                cGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCg                                                              >  1:374113/1‑250 (MQ=255)
                                                                                                                                      cGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTc                                                                                                                                                                                      <  1:221812/124‑1 (MQ=255)
                                                                                                                                              cgcACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATGTTGGCATTTCGCCGAATTTTATTGTATAGAGCAGTTCcg                                                                                                                                                                                    <  1:429108/118‑1 (MQ=255)
                                                                                                                                                         gggggCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGTCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTc                                                                                                                                                                                      <  1:155633/103‑1 (MQ=255)
                                                                                                                                                                          cGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCATGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAagag                                                                                                                            >  1:86172/1‑146 (MQ=255)
                                                                                                                                                                                aTTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCNAAANNNNNGtnnnnnn                                                                                                                  >  2:311849/1‑144 (MQ=255)
                                                                                                                                                                                aTTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTGTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGan                     <  1:311849/243‑2 (MQ=255)
                                                                                                                                                                                           gAAGGGCAGTTCTCGGCGCTGTCCTGCTCTGATTGTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAAGCACGTCTGAACAGTTACGGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGGTAAGCGTAGTTCGAAAGGTATGCAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCgg  <  1:491249/251‑1 (MQ=255)
                                                                                                                                                                                           gAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCggn                                                                                                                                   <  1:305756/122‑2 (MQ=255)
                                                                                                                                                                                           gAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGc                                                                                                                                   >  2:305756/1‑122 (MQ=255)
                                                                                                                                                                                               ggCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATATGCAATCGCGTCGGAACGTTTTCCGGCAAAAGAGAGGTAGGGACTTAACGTCATGATGGTGAGCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTta                                                       <  1:469848/194‑1 (MQ=255)
                                                                                                                                                                                                   gTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGACGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTNACGNNnnnat                                                                                                     >  2:299815/1‑144 (MQ=255)
                                                                                                                                                                                                   gTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCan                                                                            <  1:299815/169‑2 (MQ=255)
                                                                                                                                                                                                           cgcTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTccn                                                                                                                                                                                    <  1:97326/57‑2 (MQ=255)
                                                                                                                                                                                                             ntGTCCTGCGCTGATTTTGGCATTTCGCCGAAGTTGATTTtat                                                                                                                                                                                                >  1:150770/2‑43 (MQ=255)
                                                                                                                                                                                                                     cgcTGATTTTGGCATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCCCCCAACGTACCTTGATACTAGGCaa                                                                                                                                                         >  1:85144/1‑74 (MQ=255)
                                                                                                                                                                                                                          aTTTTGGCATTTCGCCGAAGTTGATGTTATTGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGGTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATTATTGTGACCTCAGTTAAGAGAAGCCAGGTATGTGTAGTTCGATATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCg                   <  1:341096/203‑1 (MQ=255)
                                                                                                                                                                                                                                ncATTTCGCCGAAGTTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAag                                                                                                                              >  1:93657/2‑90 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                       
GATTTTTCCGTTAGCGGCAAGATTGTCAAACCAGCGTTTTCCTTCTTCGACCTGTTGCGAATCGAGCACCAGCGTAAAGCCGGAGTAGCTGGCTTTTCCTGACGGCATGGCATCGCTCATCATGATGTCGCTTCCGGCAATGCGCACATTGGCGTGGGCGATGGCGGTATCGGGAAATTGCATTCCGGAAGGGCAGTTCTCGGCGCTGTCCTGCGCTGATTTTGGCATTTCGCCGAAGCTGATTTTATAGAGCAGTTCCGCGCCCAACGTACGTTGATAATAGGCAATCGCGTCGGAACAGTTACCGGCAAAAGAGAGGTAGGGACTTAACGGCATGATGGTGACCTCAGTTAAGAGAAGCCAGTTAAGTGTAGTTCGAAATTTATACAGATGAGAGGCGGCCTGATAAGACGCGCGAGCGTCGCATCAGGCAGTCGG  >  NC_000913/4325406‑4325843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑