| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,183,662 | G→A | Y261Y (TAC→TAT) | potB ← | spermidine preferential ABC transporter membrane subunit PotB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,183,662 | 0 | G | A | 100.0% | 23.2 / NA | 10 | Y261Y (TAC→TAT) | potB | spermidine preferential ABC transporter membrane subunit PotB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (6/4); total (6/4) | |||||||||||
CGCCATTGTTAGTGAATGCTGCGCTGCCTGTAACAGGCTGTCGTTGTTCATCAGCAGGCTATACCATTTGGTGGTAAAGCCCTGCCAGTTGATGCCAAAGCGCGAGCTGTTAAAGGAGTTCACAATCAAAATAATGATTGGGATATACAGGTACGCGTAGATAGCGGTCATAAAACCGCCGCGAAGCAGTCGACCGATCATTCGAGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGG > NC_000913/1183420‑1183874 | cGCCATTGTTAGTGAATGCTGCGCTGCCTGTAACAGGCTGTCGTTGTTCCTCAGCAGGCTATACCATTTGGTGGTAAAGCCCTGCCAGTTGATGCCAAAGCGCGAGCTGTTAAAGGAGTTCACAATCAAAATAATGATTGGGATATACAGGTACGCGTAGATAGCGGTCATAACACCGCCGCGAAGCAGTCGACCGATCATTCGAGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAAcc > 1:262612/1‑249 (MQ=255) ccgcGAAGCAGTCGACCGATCATTCGAGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCg < 1:141175/186‑1 (MQ=255) gcgAAGCAGTCGACCGATCATTCGAGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAACCAGCAACATCAGGcc < 1:287565/83‑1 (MQ=255) aaGCAGTCGACCGATCATTCGAGTTACACCTTCTTATTAAGCAAACGAGAAGAGCGCCAATAAACCAGCACCATCAGGCCCATAACGCTAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCAAGTCACGAATATTAAGGAACTGGACATTGATGACGTTAACGATCAGCAGGTTTTTCGCAc > 1:75095/1‑184 (MQ=255) gCAGTCGACCGATCATTCGAGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCcata > 1:186095/1‑250 (MQ=255) aGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTgg < 1:35220/251‑1 (MQ=255) nttCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGt > 1:316274/2‑85 (MQ=255) gTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAATAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGt < 2:316274/85‑1 (MQ=255) naaaCGAGAAGCGCGCCCATAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAg > 1:125539/2‑85 (MQ=255) cGAGAAGCGCGCCAATAAACCAGCAACATCAGGCCCATTAAGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAg > 1:322036/1‑149 (MQ=255) | CGCCATTGTTAGTGAATGCTGCGCTGCCTGTAACAGGCTGTCGTTGTTCATCAGCAGGCTATACCATTTGGTGGTAAAGCCCTGCCAGTTGATGCCAAAGCGCGAGCTGTTAAAGGAGTTCACAATCAAAATAATGATTGGGATATACAGGTACGCGTAGATAGCGGTCATAAAACCGCCGCGAAGCAGTCGACCGATCATTCGAGTTCCACCTTCTTATTCAGCAAACGAGAAGCGCGCCAGTAAACCAGCAACATCAGGCCCATTACGATAGTCAGCGTAATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCACCAGCAGACATCCGGCAATAATTCCCGGCATCGTCAGTGG > NC_000913/1183420‑1183874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |