Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,161,248	G→T	G61V (GGC→GTC)	fabR →	DNA‑binding transcriptional repressor FabR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,161,248	0	G	T	100.0%	49.9 / NA	18	G61V (GGC→GTC)	fabR	DNA‑binding transcriptional repressor FabR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/9);  total (9/9)

GAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCGCCGTTGC  >  NC_000913/4161055‑4161497                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          gagcattcaattATGCTCATTCTCTGTTATAGTGCCGGCAGTACTTTTGGAAAGGATCCAGACATCGTGATGGGGGAAGGAGCGCAAAAAAAAGAAAAACCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGAc                                                                                                                                                                                                  <  1:436417/240‑1 (MQ=255)                               agagCCAGAAGTATTTTGGGAAAGGTTTAAGACATCGTGATGGGGGTAAGAGCGAAAAAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGagag                                                                                                                                                                                   <  1:204317/233‑1 (MQ=255)                                                          tCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCCTTTAGCCAATTAAGTGCTGACCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                                                                                                                                                                                                                          >  1:252149/1‑170 (MQ=255)                                                                     cagacgggtgtcctccctatcaaaaGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCTTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTGATCGGCACTTCCGCGACGTAGACGAACTGGGTCTGTCCATGGTTGATGAGAGCGGTGTAATGCGACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAg                                                                                                                              <  1:224177/230‑1 (MQ=255)                                                                        gggCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGACGCATTTAGCAAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGACGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCCTGGTTGATGAGAGAGGTTTAATGCTACGCCAACt                                                                                                                                                              >  1:452450/1‑216 (MQ=255)                                                                        cagagtggtgcttttccgatcgaaaaaaaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGACAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGcn                                                                                                                           <  1:146249/228‑2 (MQ=255)                                                                                   cgcAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTACGTGCTGCACGCAGCTTCGCCCGCCTGCGTTTGCGTGAAGAGGCGCGTGAAGCGGTCATTGCTACCACCTCTTTTTATCGGCCTTTCCGCGATCTAGACGCACTGGGTCTGACCATGGTTGATGAGAGCGGTTTACTGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCg                                                                                                                                    >  1:504438/1‑231 (MQ=255)                                                                                     caacaaAAAGAAACAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCACTTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGAAGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGGGTGATCCg                                                                                                              >  1:377946/1‑251 (MQ=255)                                                                                               aaaaaaCCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCATGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTGAATGCTa                                                                                                                                                                      <  1:173268/185‑1 (MQ=255)                                                                                                               gCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGAGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCCCCTCGGTCTCCACATTTATGGAGt                                                                                    >  1:280415/1‑251 (MQ=255)                                                                                                                                 tAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTTCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTc                                                                                                        <  1:356223/213‑1 (MQ=255)                                                                                                                                 tAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATTCGCCAGGCGCGTCAgn                                                                                                                                          <  1:148276/179‑2 (MQ=255)                                                                                                                                                            cGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAAc                                       <  1:29494/251‑1 (MQ=255)                                                                                                                                                              ncAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTCGATGAACTGGGTCTGACCATGGTTGATGAGCGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCggg                                                                                                                         >  1:52483/2‑167 (MQ=255)                                                                                                                                                                 gCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCCTTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTACTGATACACCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTcc                                  >  1:463171/1‑251 (MQ=255)                                                                                                                                                                      agtgtGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGcc                                                                                                                                  <  1:350778/146‑1 (MQ=255)                                                                                                                                                                      aGTTTGCGNGNNNTNNCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCNGCGACGTAGACGAACNGGNTCTGNCCANGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGcc                                                                                                                                  >  2:350778/1‑150 (MQ=255)                                                                                                                                                                                     ncgcgTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtg                                                                                                                                                                                          >  1:146318/2‑79 (MQ=255)                                                                                                                                                                                                 ggCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACTCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGCACGCTCCGGCCCCTCCGCTGCGTTTCGTGCCGCCGTTgc  >  1:110838/1‑251 (MQ=255)                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          GAGCAATCCAGTATGTTCATTCTCTGGTATAGTGCCAGCAGTACTTTTGGCAAGGATTCAGACATCGTGATGGGCGTAAGAGCGCAACAAAAAGAAAAAACCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAACTCATGCGCCAGGCGCGTCAGCGTATCGCCAAAGGCGGGAGTGTGATCCGCACCTCGGTCTCCACATTTATGGAGTTCATCGGTAATAATCCTAACGCCTTCCGGTTATTATTGCGGGAACGCTCCGGCACCTCCGCTGCGTTTCGTGCCGCCGTTGC  >  NC_000913/4161055‑4161497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑