| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,322,325 | G→A | L208F (CTC→TTC) | trpE ← | anthranilate synthase subunit TrpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,322,325 | 0 | G | A | 95.0% | 46.7 / ‑3.3 | 20 | L208F (CTC→TTC) | trpE | anthranilate synthase subunit TrpE |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/8); total (12/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
CACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGT > NC_000913/1322098‑1322539 | cACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCg > 1:354383/1‑251 (MQ=255) gCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAAc < 1:273060/242‑1 (MQ=255) cTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCCTCGCTCTGATTACCTTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTAACGTAGTCCGTTCAGGCGAGCAGTGAAACgttgt > 1:500428/1‑175 (MQ=255) aaaaTTTCTCCAGCGCGAATCGCGTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATGCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTGTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTgn < 1:316208/207‑2 (MQ=255) gCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCtt > 1:216110/1‑198 (MQ=255) gAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGCCACGGAAACACCTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTATTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCtttttt > 1:270194/1‑173 (MQ=255) naTCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCactc > 1:96399/2‑176 (MQ=255) ggCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAATCAGGCTGGc < 1:411797/113‑1 (MQ=255) ggCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACa > 1:14543/1‑106 (MQ=255) cGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTGGCTGACGTAGTTCGTTCGGGCGGGAAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGATGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCg > 1:265902/1‑207 (MQ=255) cTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAAGCGCGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACACGGTCATCAGAGAACAGGCCGCCGAAGAAc > 1:250212/1‑251 (MQ=255) aGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACANNCTGGCCNNa > 2:26625/1‑99 (MQ=255) cTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAggn < 1:55826/155‑2 (MQ=255) tcgttCAGTTGCGGACGTAGTTCGTTCAGGCGAGCAGTGAAATGTTTTTTTTCTGCTTCATTCGGAACAAACGGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGTTCAATCACCATCAGCGTTTCAGCGAGAAAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACATTTGGGGAAAATTTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTcttcc < 1:26625/245‑1 (MQ=255) cAGTTGCTGACGTAGTTCGTTCAGGCGAGAAGTGAAACGTTGTTTTTCTCATTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGCAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGt > 1:376277/1‑250 (MQ=255) cTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATANGGGNGCTTNTTNTCTGATGGTCAATCANCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATNNTNNNCNGACAGTTg < 2:356119/165‑1 (MQ=255) cTGACGTAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAAAAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTg > 1:356119/1‑165 (MQ=255) tAGTTCGTTCAGGCGAGCAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTGTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGc < 1:451805/150‑1 (MQ=255) tcgttcAGGCGAACAGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTNNATCACCNNc > 2:27225/1‑99 (MQ=255) aGTGAAACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGc < 1:439687/41‑1 (MQ=255) | CACGTAATAGGCCGCCAGCGGTGACGGGCAGGGCAGAGAGAAACGGCGAGATGGCACCACCTGGAAAATTTCTCCAGCGCGAATCGCTTTTTGCAACAAACGCACTACGCCACCGAACTCTTCATCGCTCTGATTACATTCACAACGCATATGCGGCACGGAAACCACTGGCAGCGGCGGCGCGGCTTCGGTCAGTTGCTGACGTAGTTCGTTCAGGCGAGCAGTGAGACGTTGTTTTTCTTCTTCATTCGGAGCAAACAGGCTGGCCTGAATACGGGTGCTTTTTTTCTGATGGTCAATCACCATCAGCGTTTCAGCGAGATAAAAACAGAAATCAGGGCAGTTATTTTCCGCTGACAGTTGCGGTAAATCTTCAAATCCCGCCACAAGGTCATAAGAGAACAGGCCGCCGAAGAACATGGCTTCTCGTTCTTCCTTCGGT > NC_000913/1322098‑1322539 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |