| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 924,350 | C→T | P363S (CCG→TCG) | clpA → | ATP‑dependent Clp protease ATP‑binding subunit ClpA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 924,350 | 0 | C | T | 86.7% | 33.5 / NA | 15 | P363S (CCG→TCG) | clpA | ATP‑dependent Clp protease ATP‑binding subunit ClpA |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (8/5); minor base G (0/2); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGAAACCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCG > NC_000913/924117‑924486 | atgaGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAATCATCAAGGGCCTGAAATCGAAGTATGAAGCGcac < 1:41704/251‑1 (MQ=255) cAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTATAATTCGTGTAATTGGTTCACCACCCTATCAGGAGATCAGCAACATTTTCGAGAAAGACAGTGCTCTGGCGAGTCGATTCCAGCAAATCGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGAAAAAGCGgtg > 1:487779/1‑251 (MQ=255) gTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAg > 1:248770/1‑212 (MQ=255) gCTCTCCAGCGGTAAAATTCGTGTCATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAACTTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAACTCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGGGAAATACATTAACGATCGTCATc > 1:161047/1‑251 (MQ=255) gtgtAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATAcc < 1:200151/173‑1 (MQ=255) ccTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCTTCGCTTCCAGAAAATTGATAGTACTGATCCGTCGAGCGAATAAACTGTTCAAATCATCAATGGCCTGAAAGCGAAGTAGGAAGCGCACCACGACGTGCGCTATTCCGCAAAAGCGGGGCGTGCGGCGGTATAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGAGAAAGCCATTGATGTTATCGACGAAGCGGgc < 1:454261/251‑1 (MQ=255) gaAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGTACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGNNATCGaann > 2:42614/1‑96 (MQ=255) gaAAGACCGGGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGTACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCggg < 1:42614/222‑1 (MQ=255) cgatcgaAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGtgcgtgcg < 1:32369/92‑1 (MQ=255) cgatcgaAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGtgcgnncg > 2:32369/1‑92 (MQ=255) natcgaAGAAACTGTTCAAATCATCAATGGCCTNAAANNGACGTANNAAGCGCCNNANGACGTGCGt < 2:408169/66‑1 (MQ=255) gatcgaAGAAACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGt > 1:408169/1‑67 (MQ=255) atcgaAGAAACTGATCAAATCATCAATGGCCTGAAATCGAAGCATGAAGCGCACCACGACGTGCGTTATCCCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGAGCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTcg > 1:7341/1‑173 (MQ=255) gaaACTGTTCAAATCATCAATGGCCTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAATCGgtgcgtgc < 1:116605/83‑1 (MQ=255) ccTGAAATCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGc > 1:358582/1‑51 (MQ=255) ccTGAAAGCGAAGTATGAAGCGCACCACGACGTGCNNTNNNCNGCAAAAGc < 2:358582/51‑1 (MQ=255) | ATGAGATCCACACCATTATCGGTGCGGGTGCAGCGTCTGGTGGTCAGGTCGATGCGGCTAACCTAATCAAACCGTTGCTCTCCAGCGGTAAAATTCGTGTAATTGGTTCGACAACCTATCAGGAGTTCAGCAACATTTTCGAGAAAGACCGTGCTCTGGCGCGTCGCTTCCAGAAAATTGATATTACTGAACCGTCGATCGAAGAAACTGTTCAAATCATCAATGGCCTGAAACCGAAGTATGAAGCGCACCACGACGTGCGTTATACCGCAAAAGCGGTGCGTGCGGCGGTAGAGCTGGCGGTGAAATACATTAACGATCGTCATCTGCCGGATAAAGCCATTGATGTTATCGACGAAGCGGGCGCTCG > NC_000913/924117‑924486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |