Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,372,825	G→A	G306E (GGG→GAG)	ycjN →	putative ABC transporter periplasmic binding protein YcjN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,372,825	0	G	A	100.0%	69.5 / NA	26	G306E (GGG→GAG)	ycjN	putative ABC transporter periplasmic binding protein YcjN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/13);  total (13/13)

CACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTACTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGT  >  NC_000913/1372579‑1373038                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      nacCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTACTGCCAACACTATGCCGGGTTATAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGAGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCa                                                                                                                                                                                                                    >  1:51717/2‑250 (MQ=255)                         ccTATTACCGCGACCTTACTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCAACTATATCCTTCCGGCTGTGATTAACGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAagcagc                                                                                                                                                                                            >  1:366184/1‑250 (MQ=255)                                     cccTTACTGCCAACGCTAGGCCGGGTTCTAACGACATCATTGAAGTGAAAGACGCCTTTATGACCGGCTCCGCGCCGAGGGCGATTGACTCCACCTATATCCTTCCGGCGGTGATTAAAGAAGGCGGCCCGAAAAACGTCGTTTTCGTGGTGCCAACTGAGAAAAACTCTGCGGTCTACGGCATGTGGACCTCGCTGACCATGACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTc                                                                                                                                                                               <  1:253838/250‑1 (MQ=255)                                                                          catGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGTCGACTggg                                                                                                                                          >  1:2128/1‑251 (MQ=255)                                                                                aGTGAAAGACGCCGTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGTgcaggca                                                                                                                                                             <  1:421498/226‑1 (MQ=255)                                                                                                                 gATGGCGATTTACTCCCCCTATATCCTTCCGGCTGGGATTAAAGAAGGCGAACCAACAAACGTCGGTTTCGTGGTGCCCACCGAGACAAACTCTGCCGTCTACGGCCTGTTGACCTCCCTAACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAACCGCCGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCCGGGGCTGCGCTGCCGGTGattga                                                                                                     >  1:88204/1‑245 (MQ=255)                                                                                                                      cGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGGCGACGTCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTGAGGGAGCAGGCAGGCAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGt                                                                                                           <  1:244710/238‑1 (MQ=255)                                                                                                                               ccACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGTCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCgg                                                                                                <  1:496146/240‑1 (MQ=255)                                                                                                                                  ccTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCaaaa                                                                                                                                                                                                                 >  1:144370/1‑124 (MQ=255)                                                                                                                                         ccTTCCGGCTGTGATTAAAGAAGGCGACCCAAAAAACGGCGGTTTCGGGGTGCCAACCGAGAAAAACGCTGCGGTCTACGGCATGTTGACCCCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGAc                                                                                                                                                                                                     <  1:233251/129‑1 (MQ=255)                                                                                                                                                gCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTAGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTc                                                                                                                                                                               >  1:289449/1‑144 (MQ=255)                                                                                                                                                             aagGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGCCCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAAGTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAg                                                       <  1:234065/251‑1 (MQ=255)                                                                                                                                                                                      cggggtgGCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGCCCATTAGCGACGAGACAAAGACCGAAGAGACGGATGCAGCAGAAAAATTTGTCACGTTTATGGGGCAGGCAGACAACATTGCCGAATGGGTGATGATGTCGCCAGGTACTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACCACGACGTTATTAAGTGGCAGGGTGAACTACCgcataagc                              >  1:345582/4‑244 (MQ=255)                                                                                                                                                                                            gCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTgatgat                                                                                                                                   >  1:221746/1‑144 (MQ=255)                                                                                                                                                                                             ccAACNNAGNNNAANNCTGCGGTCTACGGCATGTTGACCNCGCTGACCATTACCGCCGAGCAAAAGACCNCNGNGACGNNAGCNGCAGAAAAATNTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATNTNGCCAGGTgctgcgctg                                                                                                                >  2:369157/1‑162 (MQ=255)                                                                                                                                                                                             ccAACCGAGAAAAACTCTGCGGGCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTgctgcgctg                                                                                                                <  1:369157/162‑1 (MQ=255)                                                                                                                                                                                                aCCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAgcagg                                                                                                                                                               >  1:21971/1‑112 (MQ=255)                                                                                                                                                                                                               ncGGTCTNNGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAgcagg                                                                                                                                                               <  2:21971/96‑1 (MQ=255)                                                                                                                                                                                                               gCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAgcag                                                                                                                                                                <  1:154380/96‑1 (MQ=255)                                                                                                                                                                                                                                  ccTCGNNGANNNTTNNCGCCGAGCAAAAGACCGAAGAGANGGAAGCAGCAGAAAAATTTGTCACCTTTANGNANCAGGNNGACNACATTGCCGANTGGGTGATGATGTCGCCAGGtgc                                                                                                                       >  2:367781/1‑118 (MQ=255)                                                                                                                                                                                                                                  ccTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAACAATTTGTCACCTTTCTGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCg                                                                                    >  1:337725/1‑153 (MQ=255)                                                                                                                                                                                                                                  ccTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGcc                                                                                                              <  1:31474/127‑1 (MQ=255)                                                                                                                                                                                                                                  ccTCGCTGACCATTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTGGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGtgc                                                                                                                       <  1:367781/118‑1 (MQ=255)                                                                                                                                                                                                                                     cgcggACCAGTACCGCCGAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAAGTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCgn                                                                                   <  1:57313/147‑2 (MQ=255)                                                                                                                                                                                                                                            cATTACCGCCGAGAAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGCGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGt  >  1:380335/1‑225 (MQ=255)                                                                                                                                                                                                                                                   gccgAGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTTCTGCGCGGCCGGTGAATAAAGCGgtggtg                                                                                           <  1:418277/129‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                      CACCAGAGATGATGCAGGCACTGACCTATTACCGCGACCTTACTGCCAACACTATGCCGGGTTCTAACGACATCATGGAAGTGAAAGACGCCTTTATGAACGGCACCGCGCCGATGGCGATTTACTCCACCTATATCCTTCCGGCTGTGATTAAAGAAGGCGACCCGAAAAACGTCGGTTTCGTGGTGCCAACCGAGAAAAACTCTGCGGTCTACGGCATGTTGACCTCGCTGACCATTACCGCCGGGCAAAAGACCGAAGAGACGGAAGCAGCAGAAAAATTTGTCACCTTTATGGAGCAGGCAGACAACATTGCCGACTGGGTGATGATGTCGCCAGGTGCTGCGCTGCCGGTGAATAAAGCGGTGGTGACTACCGCCACCTGGAAAGACAACGACGTTATTAAGGCGCTGGGTGAACTACCGAATCAGCTAATCGGTGAACTGCCAAATATTCAGGT  >  NC_000913/1372579‑1373038

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑