| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,708,813 | G→A | P555S (CCG→TCG) | eptB ← | phosphoethanolamine transferase EptB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,708,813 | 0 | G | A | 96.5% | 70.5 / NA | 29 | P555S (CCG→TCG) | eptB | phosphoethanolamine transferase EptB |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (17/11); minor base C (1/0); total (18/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGGGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGATTTTCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGC > NC_000913/3708573‑3709057 | ncAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAACATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGacac > 1:49992/2‑251 (MQ=255) tCGAATTTGGTCGGTTATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCCTCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAACGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGCGATGTGACACCCGTTGTTGTTTTCGTTAATTcca > 1:286582/1‑239 (MQ=255) naTAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACa > 1:305391/2‑250 (MQ=255) ncTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGcc > 1:146025/2‑229 (MQ=255) gTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTg > 1:33083/1‑251 (MQ=255) cAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGCAGTATAGCCAAGACAACCCCTGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCCTGTc > 1:106255/1‑251 (MQ=255) cGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTccac > 1:329068/1‑152 (MQ=255) gcATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTTATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCt > 1:179444/1‑251 (MQ=255) cATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCACAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTg > 1:390826/1‑251 (MQ=255) cATCGTACTGCGCAGATACGCCCTCCTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTAGGCTTCTTTTTTCAGATGCGCAAACGCCTg > 1:390818/1‑251 (MQ=255) cTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAGCCCATGATGGTATCGTACAGc < 1:136682/189‑1 (MQ=255) naGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCATCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCgg > 1:91865/2‑251 (MQ=255) ccctgaagaGCAAAATGGCTTGTGAGCGGCGAGAAAGTCAGCAGGCCGCTTAGGTAGCCGCTGCCTCTGGTGGCTGCTAGATGTGACACCAGTTGTTGTTGTCGTTAATTCCATCAGCCGGTGAAGTATAGCCAAGACAACCTAGGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACGTTCAGGGAAGCGGCGTTTTGGAGCTGCGCAATCGCCTGCGCATTGGCCGGATTTTCCAGATATTTATCt < 1:255553/243‑1 (MQ=255) aTAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGATTTTCCAGATATTTATCTGACATc < 1:6947/251‑1 (MQ=255) cAAAATGCATTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTgg > 1:426261/1‑218 (MQ=255) ncTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGATTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGATTTTCCa > 1:92880/2‑223 (MQ=255) ccTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGc < 1:322427/176‑1 (MQ=255) ngagaAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGCAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGATTTTCCAGATATTTATCTGACa > 1:99022/2‑226 (MQ=255) cagGCCGCTTAGTTAGCCTCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGTCAACCCATGATTGTATCGTACAGCTCTACGTGACGGCTTGGCACTTTCATGTCGGTTTCTTTTTTCATCTTCGCAAACGCCTGCGCATTGGCCGGATTTTCCAg < 1:87891/200‑1 (MQ=255) ncGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGtata > 1:311781/2‑85 (MQ=255) ccGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGtata < 2:311781/85‑1 (MQ=255) gCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACAcc < 2:26462/43‑1 (MQ=255) gCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGAGATGTGACAcc > 1:26462/1‑43 (MQ=255) gttagCGGCTGCCTCTTTTGCCTGCGAGATGTGACACCGGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATCGCCAAGACAACCCATGATGGCATCGTACAGCTCTACGTGACGGCGAGGCGCTTTCATGTCGGCTTCTTTTTTCa > 1:423388/1‑152 (MQ=255) gCNGCTNCNNCNNTTGNCTGCGAGATGTGACACCAGTTNNTGTTTTCGTNAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACANCTCTACGTGNNGGNGTGGCNCNNNCNNNTCNGCTTCTTTGTTCAGCTGCGNAANNGCCGGCNCATTGGCCGGATTTTCCAGATATTTa < 2:106255/192‑1 (MQ=255) gCCGCTGCCTCTTTTGCCTGCGAGATGTGACACCAGTTGTTGTTTTCGTTCATTCCACCATCCGGTCAAGTATAGCCAAGACAAACCATCATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCa > 1:227205/1‑130 (MQ=255) ctTTTGCCTGCGAGATGTGACACCAGGTGTTGTGTTCGTTAATTCCACCAGCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGGGACGGCGTGGCACGTTCATGTCGGCGTCTTTTTTCAGCTGCGCAAACGCCTGCGCAGTGGCCGGATTTTCCAGATATTTATCTGACATCCAGAc < 1:236004/196‑1 (MQ=255) ccTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGATTTTCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTgn < 1:59038/251‑2 (MQ=255) ccTGCGAGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTGTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGAGTTTCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCcggcg < 1:228844/219‑1 (MQ=255) | GCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTCGAACCTCCGACCCCTTCGTCCCGAACGAAGTGCGCTACCAGGCTGCGCCAATCACCGAATGCGGGGCGCATCTTACTGCGCAGATACGCCCTCGTCAATCCCTTAATAGCAAAATGCCTTTTGATCGGCGAGAAAGTCAGCAGGCCGCTTAGTTAGCCGCTGCCTCTTTTGCCTGCGGGATGTGACACCAGTTGTTGTTTTCGTTAATTCCACCATCCGGTGAAGTATAGCCAAGACAACCCATGATGGTATCGTACAGCTCTACGTGACGGCGTGGCACTTTCATGTCGGCTTCTTTTTTCAGCTGCGCAAACGCCTGCGCATTGGCCGGATTTTCCAGATATTTATCTGACATCCAGACCATCATCGGTACGCGGAACTGCTCCGGCGGTGCCAGTTCACGCGGCGTGCCGTGCAGGTGC > NC_000913/3708573‑3709057 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |