| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 553,298 | C→T | V215I (GTA→ATA) | lpxH ← | UDP‑2,3‑diacylglucosamine diphosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 553,298 | 0 | C | T | 93.3% | 30.1 / NA | 15 | V215I (GTA→ATA) | lpxH | UDP‑2,3‑diacylglucosamine diphosphatase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (6/8); minor base G (0/1); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAAC > NC_000913/553075‑553503 | cgcgCCGGATTATTGCGGGAAGACAGTCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTAAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACTTCATCCGCCGTGACTTTCACCATTGATCCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGc < 1:331047/251‑1 (MQ=255) ccgatttcTTTAGAGGGCACAGAAAATAGCACGGAAAGAGAGCAAGGAAAAGGGTGGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAAGGAATCGGCTTAACTTCATCCGCCGGTACTGTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGGGAAAAGCAGGTTGGTTAGTTGCGATAAGTTCATTCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGc < 1:473644/243‑1 (MQ=255) ngCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTg > 1:300633/2‑250 (MQ=255) gCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGCAATCAGCCAAGTTGCGGGTTTTTTCAAACGGAAAATGAATCAGCTCAACGTCATACGCCGTGACTTTCACAATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATgggaa > 1:226556/1‑221 (MQ=255) cgtgttctcttcccatctaGGAAAACGGTTGCTTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCCCCCAGTATCACGCGAAAAGCAGGTTGCTGAGTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGacc < 1:453258/233‑1 (MQ=255) cGGAAAGCGAGCAAGGACAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATg > 1:401785/1‑200 (MQ=255) gtgAAATCAGCAAAGTGGCGGGTTTTCAAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCg < 1:86980/135‑1 (MQ=255) aaaaGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTc < 1:12207/222‑1 (MQ=255) ttGCGGGTTTTTTAAAGCGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTc < 1:199448/128‑1 (MQ=255) gAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAACAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGAt > 1:264553/1‑215 (MQ=255) aaTGAATCAGCTCAACTTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTAGCACGTGAAAAGCAGGTTGCTGATGGGCGATAAGTTCATGCACCGCCTGGCGATGGGTAGGCCCGTGTAGCAGCCATTGCTCCTGATGTGTTTCCATCGCACTGAGCACCGGGTTGTGGTGAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTcgc < 1:477116/251‑1 (MQ=255) cATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTg < 1:9354/73‑1 (MQ=255) cATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTg > 2:9354/1‑73 (MQ=255) ccgTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCacaaac < 1:433782/251‑1 (MQ=255) naaCCTTCCGTATGCCAGGCACCCAGTATCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGa > 1:404320/2‑97 (MQ=255) | CGCGCCGGATTATTGCGGGAAGACATGCGTCTTAAAACTCCTGTGGTGCACAACTCTCGGCTTTAGAGGGCACAGAGAATAGCACGGAAAGAGAGCAAGGAAAACGGTTGCGTGGCTGTGAAATCAGCAAAGTTGCGGGTTTTTTAAAACGGAAAATGAATCAGCTCAACGTCATCCGCCGTGACTTTCACCATTGAACCTTCCGTATGCCAGGCACCCAGTACCACGCGAAAAGCAGGTTGCTGATTGGCGATAAGTTCATGCACCGCCGGGCGATGGGTATGCCCGTGGATCAGCCATTGCACCTGATGTTTTTCCATCGCACTGACCACCGCGTTTTGGTTAACGTCCATGATCGCCAGCGATTTACTGCTGTTGGCTTCTTTGCTGTTCGCGCGCATTCGCGCGGCAATGCGTTTGCGCACAAAC > NC_000913/553075‑553503 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |