| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,652,331 | 0 | T | C | 94.4% | 53.1 / NA | 19 | intergenic (+2346/+397) | ydfD/ynfP | lysis protein/protein YnfP |
| Reads supporting (aligned to +/- strand): ref base T (1/0); major base C (9/8); minor base A (1/0); total (11/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01 | |||||||||||
GGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑TTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGC > NC_000913/1652144‑1652533 | ggaggaCATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCCTCTTATTAAGCCAGCAATCGACGCCCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCACCTTTTTTTTTCAACCTGAAGTGTc > 1:171254/1‑249 (MQ=255) aaaaTGGAACAATGATGATCAGGAGAAATTTAGCAAACGACCGTTTCACGGTCCCAAAAACCCAGGCGGGAACCAATCGGGCCATTTATCTTTTTAAACCAGCCATCGACGCTCTCCCGAGTCAGATCACACTAACGAGACTGAGCAAATAGCATATCATTGATGTTCAA‑CTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGAACCTTTGTTTTTCCACCTGAAAAGTCCGCGAGAGGAAAAAATTAt > 1:271431/1‑251 (MQ=255) cAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTg < 1:31295/251‑1 (MQ=255) ncGTTTCACGGTACCAACAAAAAAGGCGGGAACCAATTGGGTCCTTCATCTTTTTAAGCCAGCCATTGACGCTCTCCGGAGTCCGATGGCATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCACCTTC‑GAGGGTATGGGAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAATGTCAGCGAGGGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATACAGCAGATGTggg > 1:147222/2‑251 (MQ=255) ccGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGTAGTCAGATGACATTAACGAGACTGATCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATTCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGTTGACCATTTTACCGTTGACTCAATAAGGCAGATGTggg < 1:464869/251‑1 (MQ=255) caGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGa < 1:2263/194‑1 (MQ=255) ggCNGNNANNAATNGGGTCATTCATCTTATTAAGCNNGCAATCGACNCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATNTTCAC‑CTCANNGANTATGGNANNNCNNNAANACAAAAATGCACCTTTGTTNTTNNACCTGANGTGTCAGCGAGAGTAAAAAATTATgg < 2:119832/189‑1 (MQ=255) ggCGGGAAACAATCGGGTCATTCATCATATTTAGCCAGCAATCGACGCTCTCCGGAGGCAGATGACATTAAAGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGGATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTTAACCTGAAGTGTCAGCGAGAGGAAAAAATTATgg > 1:119832/1‑189 (MQ=255) gTCATTCATCTTATTAAGCCAGAAATCGACGCTCTCCGGAGTCAGCTGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑ATAAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAACAGTGCCGGACTCAGCCATCGAAAATc > 1:383856/1‑251 (MQ=255) aGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTGCAACCTGAAGTGTCAGCGagag < 1:136411/144‑1 (MQ=255) nccAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACAt > 1:52145/2‑250 (MQ=255) cGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTTATGTTCAC‑CTCAGAGAGTATGGCAAAACAGAAAAACAAAAATGCACCTGTGTTTTTCAACCTGAAGTGGCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGcc < 1:431244/250‑1 (MQ=255) ngCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCATTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCctgct > 1:46167/2‑251 (MQ=255) cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCgg > 1:18155/1‑191 (MQ=255) cAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGc > 1:428419/1‑251 (MQ=255) gagaCTGAGCAAAGAGCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAATGCAGATGTGGGAc < 1:386193/157‑1 (MQ=255) agagCATATCATTGATGTTCAC‑CTCAGAGAGTATGGCAGAAAAGAAAACCAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCGGCGCGAGTAAGAAATTATGGTGTCCATTTTACCGTTGACTCAATAAGGTAGATGTGGGACGCAGCGa > 1:325868/1‑152 (MQ=255) tGTTCAC‑CNCNNNGNNTATGGCAGAACAGAAAAACCAAAATGCACCTTTGTTTTTCAACCNGCAGTGTCAGCgag > 2:347584/1‑75 (MQ=255) ggTTCAC‑CTCAGAGAGTATGGCATAACAGAAAAACAAAAATGCACCGTTGTTTTTCAACCTGAAGTGTCAGCgag < 1:347584/74‑1 (MQ=255) | GGAGGACATAGATCTGAAAAATGGAACAATGATGATCAGGAGAAATTTAGCAAAAGACCGTTTCACGGTACCAAAAACACAGGCGGGAACCAATCGGGTCATTCATCTTATTAAGCCAGCAATCGACGCTCTCCGGAGTCAGATGACATTAACGAGACTGAGCAAAGAGCATATCATTGATGTTCAC‑TTCAGAGAGTATGGCAGAACAGAAAAACAAAAATGCACCTTTGTTTTTCAACCTGAAGTGTCAGCGAGAGTAAAAAATTATGGTGACCATTTTACCGTTGACTCAATAAGGCAGATGTGGGACGCAGCGATAAAACGTGCCGGACTCCGCCATCGAAAATCATATCAGTCGAGACATACTTATGCCTGCTGGTCGCTGACAGC > NC_000913/1652144‑1652533 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |