Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,476,285	G→A	H52H (CAC→CAT)	yheO ←	DNA‑binding transcriptional regulator YheO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,476,285	0	G	A	100.0%	51.2 / NA	20	H52H (CAC→CAT)	yheO	DNA‑binding transcriptional regulator YheO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (14/6);  total (14/6)

CACAGCAGACCAATTACACGCTGTTCGCGGTTACGAATCGCGATAGTCAGGGACTTCATTAATACGCCGCTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGTGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGG  >  NC_000913/3476055‑3476527                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   cacaGCAGACCAATTACACGCTGTTCGCGGTTACGAATCGCGATAGTCAGGGACTTCATTAATACGCCGCTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTg                                                                                                                                                                                                                                >  1:397626/1‑251 (MQ=255)       agACCAATTACACGCTGTTCGCGGTTACGAATCGCGATAGTCAGGGACTTCATTAATACGCCGCTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGcc                                                                                                                                                                                                                          <  1:29801/251‑1 (MQ=255)                              gTTACGAATCGCGATAGTCAGGGACTTCATTAATACGCCGCTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTca                                                                                                                                                                                                    >  1:15029/1‑249 (MQ=255)                                                       ttCATTAATACGCCGCTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAg                                                                                                                                                                                                                            <  1:355851/201‑1 (MQ=255)                                                                 cgccgcTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTACTATAGTGCAGCATACGCAGCGCACGGTCAGTACTTGGCGACCAAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACCTTTTAGATACTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCg                                                                                                                                                                >  1:279345/1‑251 (MQ=255)                                                                                                     cGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAAc                                                                                                                            >  1:217039/1‑251 (MQ=255)                                                                                                      gctgctATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGACAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCAACGTTAGAAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCGCAGCGGGAGCCAATAAGCATCGATAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAGGGACGTTGATCCAGTAAATCCAACt                                                                                                                           >  1:486297/1‑251 (MQ=255)                                                                                                      gctgctATCCGCTCCCGTCATATCGTGCAGCATACGTAGAGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAACCGATTTCACAGCGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACt                                                                                                                           >  1:486298/1‑251 (MQ=255)                                                                                                         gctATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGACAATTTTAGATCCTGCAAAGAATGAAAAACGCTTTCACAGTGGGAGcc                                                                                                                                                                                                                          >  1:252181/1‑153 (MQ=255)                                                                                                                   cccGTAATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAg                                                                                                                                                                                                                    >  1:397034/1‑149 (MQ=255)                                                                                                                              gTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGCATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTTCGTTgg                                                                                                            >  1:213948/1‑242 (MQ=255)                                                                                                                                          tAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTNACCNNNnnac                                                                                                                                                                                                  >  2:309759/1‑144 (MQ=255)                                                                                                                                          tAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTTCGTTgn                                                                                                            <  1:309759/230‑2 (MQ=255)                                                                                                                                                   gTCAGTAATTGGTGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGTTACGTAGGATTTCAGAatatn                                                                                                                                                                       <  1:122069/162‑2 (MQ=255)                                                                                                                                                                                                 gCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGCCTTCACCGTGGGAGAAAATAAGAATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCACAATCGGTCTGGTCGACAGGACGTTGATCCAGTAAATCCAACTCACTGGTTTCGTTGGTTaaaa                                                                                                      >  1:497808/1‑181 (MQ=255)                                                                                                                                                                                                            tgCTGAACATTTTAGATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGATCCTGTCGTTAACTTTTc                                               <  1:114836/224‑1 (MQ=255)                                                                                                                                                                                                            ggCTGTACCTTTTAGATCCTGCAACGNATACACNNCGNTTTCACAGTGGGAGCCAATNAGNNNCNNNANCCCGTNCANNACCGCTTCGNAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAANCCCACTCACNNGTTTCGTTGGTTAAAAGCGACNTGGNNANNanaaanaan                                                                               >  2:114836/1‑192 (MQ=255)                                                                                                                                                                                                                     ttttAGATCCTGCAAAGAATGCAAAACGATGTCGCAGTGGGAGCCAATAAGCAGCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGAc                                                                                                                                                <  1:253474/119‑1 (MQ=255)                                                                                                                                                                                                                          gATCCTGCAAAGAATGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGACAGGACGTTGATCCAGTATATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGCCATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAAc                                                    >  1:237108/1‑206 (MQ=255)                                                                                                                                                                                                                                ggaaaGAATGCAAAACGATTTCACAGTGGGAGCCAATACGCATCGCTAAGCCGTCCAACACAGCTTCGTAGGATTTCAGAATATCAAACTCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCCACTCACTGGTTTCGTTGGTTAAACGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCgg  >  1:483210/3‑250 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                   CACAGCAGACCAATTACACGCTGTTCGCGGTTACGAATCGCGATAGTCAGGGACTTCATTAATACGCCGCTTTTGGCGCGAGTAAAGTAGCATTTAGAAACGCTGCTATCCGCTCCCGTCATATCGTGCAGCATACGTAGCGCCAGGTCAGTAATTGGCGAACCAATCTTCCGGCCTGTATGTTCACCGTTAGCAATGCGAATGGCTGAACATTTTAGATCCTGCAAAGAGTGCAAAACGATTTCACAGTGGGAGCCAATAAGCATCGCTAACCCGTCCACCACCGCTTCGTAGGATTTCAGAATATCAAAATCGGTCTGGTCGAAAGGACGTTGATCCAGTAAATCCAACTCACTGGTTTCGTTGGTTAAAAGCGACCTGGACATGAAAAAAAACACTCCTTTTCAGGAGCCTGTCGTTAACTTTTCAGGGCAGGCTCATTAATGATGCGGGTAACTAAATTAATACAGCGG  >  NC_000913/3476055‑3476527

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑