Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,880,433	G→A	R206* (CGA→TGA)	casD ←	type I‑E CRISPR system Cascade subunit CasD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,880,433	0	G	A	100.0%	52.0 / NA	19	R206* (CGA→TGA)	casD	type I‑E CRISPR system Cascade subunit CasD
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/11);  total (8/11)

GCGCTGACTGCAATAAAACATGACAGCCTTCTGGTGTGTTTCGCTTCTCAACATGAAAAAGAAAATCACGAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACC  >  NC_000913/2880216‑2880665                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         gcgcTGACTGCAATAAAACATGACAGCCTTCTGGTGTGTTTCGCTTCTCAACATGAAAAAGAAAATCACGAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCg                                                                                                                                                                                                          <  1:195070/250‑1 (MQ=255)       aCTGCAATAAAACATGACAGCCTTCTGGTGTGTTTCGCTTCTCAACATGAAAAAGAAAATCACGAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTaaa                                                                                                                                                                                                      >  1:12894/1‑248 (MQ=255)                          gCCTTCTGGTGTGTTTCGCTTCTCAACATGAAAAAGAAAATCACGAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACGGTACTGAGATACATCCATACCTCCGTTAATCACATGCCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTtcgtag                                                                                                                                                                                                                 <  1:467273/218‑3 (MQ=255)                                                             gAAAATCACGAGCAGCATCCGGTCTGNTTGGAANNAANTGCCATAATCCCTGGTGAANTTNNNANNNANACCTGNTCNNGGCCCTGGCNATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCNGGAAGCAAANNGTCACGGCAAGGTGATCATCGNTTCNNCNNGNACCNTan                                                                                                                                                                                                       >  2:119359/1‑192 (MQ=255)                                                                     cGAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAAATGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAAttt                                                                                                                                      >  1:107140/1‑250 (MQ=255)                                                                      gAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAATATCCCTGCTCCAGGCCCTGGCAATGATGTCTTTACTTAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCggn                                                                                                                                                                                                                       <  1:99486/168‑2 (MQ=255)                                                                           gcaTCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAgcg                                                                                                                               <  1:119359/251‑1 (MQ=255)                                                                                                aaatGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGcn                                                                                                           <  1:95435/250‑2 (MQ=255)                                                                                                 aatGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCtn                                                                                                         <  1:55088/251‑2 (MQ=255)                                                                                                    gCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCGCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGGTCGTCGCTCGCCTTAAATTTTAAAGGATGCCCTGTAACTGATTCCTCTCTATGTATATCGCCGCGAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACa                                                                                                      <  1:239292/251‑1 (MQ=255)                                                                                                     ncATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGTCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTGAATCACATACCATTTTCGGGAAGCAAATTGTCAAGTCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAAt                                                                                                                                   >  1:122435/2‑221 (MQ=255)                                                                                                                          gTAAAGATCCCTGCTACAGACCCAGGCCATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGCAGCAAATTGTCAAGGAAAGGCGATAATCGGATCGTCGCGCGCCGCACCTTTTAAATGATGCCCTGTAACTGAt                                                                                                                                                                             >  1:412520/1‑158 (MQ=255)                                                                                                                                                           gaCTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTcc                                                             <  1:135489/237‑1 (MQ=255)                                                                                                                                                                tACTGNNATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTaaa                                                                                                                                                                                                      <  2:12894/95‑1 (MQ=255)                                                                                                                                                                           aTCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCGGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTTACATGTCCCCAAAAAAAGCGGGTGTGTTAGtn                                                                         <  1:49498/209‑2 (MQ=255)                                                                                                                                                                                 aCCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCGCGCGCCg                                                                                                                                                                                                          <  1:137656/74‑1 (MQ=255)                                                                                                                                                                                      ctTTAATCACATACCATTCTCGGGAAGCAAATTGTCAAGCCAAGGTGATCATCGGTTCGTCGCGCGCCGTACATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGACGCCAACGGGCTCATAATTTAATAGCGCCTTTTGAGGATCCGATGCCTGACATGTCACCAAAAAAAGCGGGGGTGTTAGTggg                                                                       >  1:507227/1‑200 (MQ=255)                                                                                                                                                                                                         tcGGGAAGCAAATTGTCAAGGCAAGGCGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGCTGCCCTCTCACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCACAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAAcc  >  1:478948/1‑250 (MQ=255)                                                                                                                                                                                                            ngAAGCAAATTGTCAAGGCAAGGTGATCATCGGTTCGTCCCGCGCCGTACATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCaaaa                                                                                           >  1:49767/2‑158 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                                                                                         GCGCTGACTGCAATAAAACATGACAGCCTTCTGGTGTGTTTCGCTTCTCAACATGAAAAAGAAAATCACGAGCAGCATCCGGTCTGTTTGGAAATAAATGCCATAATCCCTGGTGAAGTTGGTAAAGATCCCTGCTCCAGGCCCTGGCAATGATGACTTTACTGAGATACATCCATACCTCCTTTAATCACATACCATTCTCGGGAAGCAAATTGTCGAGGCAAGGTGATCATCGGTTCGTCGCGCGCCGTAAATTTTAAATGATGCCCTGTAACTGATTCCTCACTATATATATCGCCGCCAACGGGCTCATAATTTAATAGCGCCTTCTGAGGATCCGATGCCTGACATGTCCCCAAAAAAAGCGGGTGTGTTAGTGGGCAACTTCTCCGCCCCAGGTAAGGTGTATACCGAGGCTTTAATACTGCTTTTTCAAGTTCTGAGATAACC  >  NC_000913/2880216‑2880665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑