Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,363,627	C→T	F295F (TTC→TTT)	puuC →	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,363,627	0	C	T	100.0%	36.7 / NA	14	F295F (TTC→TTT)	puuC	gamma‑glutamyl‑gamma‑aminobutyraldehyde dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/4);  total (10/4)

GGGTTTTGGTCATGAAGCCGGGCAGGCGCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTAATCGACTGCGCCCACGCCGACTCGGTCCATAGCTTTATTCGGGAAGGCGAAAGCAAAGGGCAACTGTTGTTGGATGGCCGTAACGCCGG  >  NC_000913/1363405‑1363863                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             gggTTTTGGTCATGAAGCCGGGCAGGCGCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTTTACAACCAGGGACAGGTGTGc                                                                                                                                                                                                                         <  1:412014/244‑1 (MQ=255)                                                      ncATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTTTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGtt                                                                                                                                                              >  1:101596/2‑250 (MQ=255)                                                             cTTTACCGGGTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTAGCTGGACGCGGGCGGCACAAGCGCCAACATCGTTTTCGCTGCCTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGAAGCAGGCATTTTTTATAACCAGGGCCAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGCATTCTTAGCACTGTTAAAAcagc                                                                                                                                                      >  1:438186/1‑251 (MQ=255)                                                                                                                              gcgTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGGTGTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCGCCGCAGCAGGCATTTTTTACAAGCAGTCACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGATAGCATCGCCGATGAATTCTTGGCCCTGTTAAAACAGCAGGGGCAAAGCTGGCTGCCGTGCCATCCACCGGATCCCGCAACAACCAGGGGCACCTTAATCGAc                                                                                     <  1:455293/251‑1 (MQ=255)                                                                                                                                gTCTGNNTGNNNGCNNGCGGCAAAAGCGCCAACATCGTTNTCGCTGACTGCCCGGATTTGCAACAGGCGNCNANCGCCNNCGCNGCAGGCATTTNTTACAACCAGGGCCAGGTGTGCATCGCCGGACCGCGCCTGTTg                                                                                                                                                                                                    >  2:366203/1‑138 (MQ=255)                                                                                                                                gTCTGGCGGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCGGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGTCATTTTTTACAACCAGGGACAGGTGTGCATCGCCGTAACGCGCCTGTTg                                                                                                                                                                                                    <  1:366203/138‑1 (MQ=255)                                                                                                                                      ctggAAGCGGGCGGAAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGAAACAGGCGGCAAGCGCCACCGAAGAAGGCATTTTTTACAACCAGGGACAGGTGTGCATCGCCGGAACGAGCCTGTTGCTGGAAGAGAGCATCGcc                                                                                                                                                                                  >  1:64788/1‑150 (MQ=255)                                                                                                                                        ggAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGAGCCACCGCAGCAGGCATTTTTTACACCCAGGGACAGGGGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAAcag                                                                                                                                                       >  1:226145/1‑175 (MQ=255)                                                                                                                                                            ccAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTTTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGCAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGACACCTTAATCGACTGCGCCCACGCCGACTCGGTCCATAGCttt                                                       >  1:83216/1‑251 (MQ=255)                                                                                                                                                                          gCTGACTGCCCGGCTTTGCAACAGGCGGACAGCGCCACCGCAGCAGGCATTTTTTACAACCAGGGACAGGTGTGCATAGCCGGAACGCGCCTGTTGCt                                                                                                                                                                                                  >  1:152904/1‑98 (MQ=255)                                                                                                                                                                                                  gcggcNNGCNNNACNNCAGCAGGCATTTTTTACAACCAGNGACAGGTGTGCATCGCCGGAACGCGCCTGNTNCNGGAANNGAGNATCGCCGATGNATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCANCNACTTGATCCCGCAaccac                                                                                                        >  2:371842/1‑164 (MQ=255)                                                                                                                                                                                                  gcggcAAGCGCCACCGCAGCAGGCATTTTTTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAaccac                                                                                                        <  1:371842/164‑1 (MQ=255)                                                                                                                                                                                                     ncAAGCGCCACCGCAGCAGGCATTTTTTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGAt                                                                                                                                                                               >  1:95953/2‑90 (MQ=255)                                                                                                                                                                                                                 gcagcaGGCATTTTTTACAACCAGGGACAGGTGTGCATAGCCGGAACGCGCCTGTTGCTGGAAGAGAGAATCGACGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCACCCACCATGGGCACCTTACTCGACTGCGCCCACGCCGACTCGGTCCATAGCTTTATTCGGGAAGGCGACAGCAAAGGGCAACTGTTGTTGGATGGCCGTAACGCCgg  >  1:225313/1‑251 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             GGGTTTTGGTCATGAAGCCGGGCAGGCGCTGTCGCGTCATAACGATATCGACGCCATTGCCTTTACCGGTTCAACCCGTACCGGGAAACAGCTGCTGAAAGATGCGGGCGACAGCAACATGAAACGCGTCTGGCTGGAAGCGGGCGGCAAAAGCGCCAACATCGTTTTCGCTGACTGCCCGGATTTGCAACAGGCGGCAAGCGCCACCGCAGCAGGCATTTTCTACAACCAGGGACAGGTGTGCATCGCCGGAACGCGCCTGTTGCTGGAAGAGAGCATCGCCGATGAATTCTTAGCCCTGTTAAAACAGCAGGCGCAAAACTGGCAGCCGGGCCATCCACTTGATCCCGCAACCACCATGGGCACCTTAATCGACTGCGCCCACGCCGACTCGGTCCATAGCTTTATTCGGGAAGGCGAAAGCAAAGGGCAACTGTTGTTGGATGGCCGTAACGCCGG  >  NC_000913/1363405‑1363863

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑