| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,111,727 | 2 bp→TT | coding (865‑866/2544 nt) | opgH → | osmoregulated periplasmic glucans biosynthesis protein H |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,111,727 | 0 | C | T | 85.7% | 26.0 / NA | 14 | P289M (CCG→TTG) | opgH | osmoregulated periplasmic glucans biosynthesis protein H |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/7); minor base G (0/2); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | NC_000913 | 1,111,728 | 0 | C | T | 92.9% | 28.7 / NA | 14 | P289M (CCG→TTG) | opgH | osmoregulated periplasmic glucans biosynthesis protein H |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/8); minor base G (0/1); total (5/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGG > NC_000913/1111526‑1111952 || ggTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGAACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACCCTTTGCTGTATACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTg > 1:479200/1‑250 (MQ=255) aaTACAGTATATCTGTGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTAGGAATCAGTAAAAGCCACCGTGAATGCCAAACACTTTTATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGc < 1:189255/251‑1 (MQ=255) cAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCTTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACGTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTTAAGTCGTTGGCGAAGGTCAGATTTTCTATcgc < 1:391595/251‑1 (MQ=255) cGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGGGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATTTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGTAGCTTATCGCTGAAGTCGGTGGAGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCgg < 1:350384/251‑1 (MQ=255) gCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGc < 1:202685/194‑1 (MQ=255) gggAATCAGTCAAAGCCACCGGGAATGGCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATACCTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGc > 1:427704/1‑115 (MQ=255) gggAATCAGGAAAAGCCACCGGGAATTCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTAGAAGTGGGATATCGGGGTCGAAGAGCAAAAAGCCTGGATGGAGCTTATCGCTTAATGCGGGGGCGGAGGTCAGATTTTCTATCACCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGTTAATATc < 1:75313/188‑1 (MQ=255) gAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGCTATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATg > 1:461632/1‑190 (MQ=255) annaGCCACNNGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCt < 2:18300/99‑1 (MQ=255) aaaaGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCt > 1:18300/1‑99 (MQ=255) ccACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGACGGTACGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGAGGCAGCAAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGAt > 1:284831/1‑251 (MQ=255) cAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACGTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGGCGGTGGCGAAGGTCAGATTGTCGATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATg < 1:64949/164‑1 (MQ=255) aaaCACTTTGATGTCTACATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAG‑TAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCgn < 1:404556/176‑2 (MQ=255) aCATTCTTAGTGACAGTTATAACTTGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGAGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATgg < 1:389842/249‑1 (MQ=255) || GGTCGCGATAAATACAGTATATCTGCGTCAACAGTTGGCGATGAACCATTAAACCCGGAGCATCGCACGGCGTTGATCATGCCTATCTGTAACGAAGACGTGAACCGTGTTTTTGCTGGCCTGCGTGCAACGTGGGAATCAGTAAAAGCCACCGGGAATGCCAAACACTTTGATGTCTACATTCTTAGTGACAGTTATAACCCGGATATCTGCGTCGCAGAGCAAAAAGCCTGGATGGAGCTTATCGCTGAAGTCGGTGGCGAAGGTCAGATTTTCTATCGCCGCCGCCGTCGCCGCGTGAAGCGTAAAAGCGGTAATATCGATGACTTCTGCCGTCGCTGGGGCAGCCAGTACAGCTACATGGTGGTGCTGGATGCTGACTCGGTAATGACCGGTGATTGTTTGTGCGGGCTGGTGCGCCTGATGG > NC_000913/1111526‑1111952 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |