Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 371,176 G→A E300E (GAG→GAA)  mhpB → 3‑carboxyethylcatechol 2,3‑dioxygenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913371,1760GA100.0% 57.3 / NA 23E300E (GAG→GAA) mhpB3‑carboxyethylcatechol 2,3‑dioxygenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (13/10);  total (13/10)

TGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTAC  >  NC_000913/370945‑371384
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                
tgttgAGGATCAGATAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGc                                                                                                                                                                                                <  1:285092/250‑1 (MQ=255)
            gagaACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGcc                                                                                                                                                                                    >  1:350313/1‑250 (MQ=255)
                                          ngATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTg                                                                                                                                                                      >  1:303504/2‑234 (MQ=255)
                                                                                 ncAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAg                                                                                                                           >  1:148992/2‑238 (MQ=255)
                                                                                                      tAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTACGTTGGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCGAAGCCCGGAATGTATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGc                                                                                                                <  1:490077/228‑1 (MQ=255)
                                                                                                                              nnnnnngTNNNNNCATNAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTCAAGCGCCAGCACAGAGAACTg                                                                                                                                                                      <  2:303504/144‑1 (MQ=255)
                                                                                                                               ggAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGatgatg                                                                                                                                                 >  1:446084/1‑170 (MQ=255)
                                                                                                                                gAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAg                                                                                                                                                                                  >  1:200533/1‑136 (MQ=255)
                                                                                                                                   aGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCtn                                                                                                       <  1:57930/208‑2 (MQ=255)
                                                                                                                                    gTCGACACAGGAAATCAAACCCGGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACGGGCGAAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTGGGCTCGTTAAGCCCCAAAACAGAAAACTGAATATGCAGGAAAAGATGAGGAGTTATCAGCCACAAACCGAAgccg                                                                                                                        <  1:195855/190‑1 (MQ=255)
                                                                                                                                       nacacATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTg                                                        >  1:53152/2‑251 (MQ=255)
                                                                                                                                          acaTGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGc                                                                                                                                       >  1:261549/1‑169 (MQ=255)
                                                                                                                                           caTGAAATCAAAACCTGGGTCGCCGCTTTGGCCGCTATTTCTGCGTTTGTCAACTGGCGGATCGAATTGCGGTAGTCCCTCCCAATCCCGGAATGGATTGCCGTATTTGGCTCGTTAAGCGCCAGAACagag                                                                                                                                                                           <  1:235727/132‑1 (MQ=255)
                                                                                                                                               aaaTCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCCTTTGGCAACTGGCGTAGCGAAGGGAGTTATTACCGCCCAATCCCGGAATGGATTGACGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGCTGATGAGTTATCAGCAACAAACCGAAGCCGCCAACAGCCGTTTTCTGAATGTAGAAGAAGCGGGCAAAACGCTGCGCATCCATTTTAATGACTGCGGACa                                                  >  1:467888/1‑249 (MQ=255)
                                                                                                                                                  tCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTATGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCCATCCCGGAATGGATTGCCGGa                                                                                                                                                                                                      >  1:165738/1‑98 (MQ=255)
                                                                                                                                                               ngccgcTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGAGAAGGGCGTTATTACCGCACAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAAACGTTGt                                >  1:93090/2‑251 (MQ=255)
                                                                                                                                                               agacgtgtgctcttctgaccttGCGGTTGGCAATGGGCGTAGCGAAGGGCGTTAGTACCGCCCAATCGCGGAATGGATTGCCGTATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGATAAGATTATGACTTACCAGTCACAATCCTAGGCCGCCACCAGCCGTTTTCTAAATGTAAAAGAAGCCGGTAAAACGCGTCCCATCCATTTTAATGTCTGCCGGCAAGGCGACGAAACCGTTg                                 <  1:430282/229‑1 (MQ=255)
                                                                                                                                                                          ccGCTGTTTCTGCGTTTGGCAACTGANGTCGCGNNGGNCGTTATTACCGCCCATTTCNGGNNNGNNNTNTTGGANTTNNCTCCTTAAGNGCCAGTCCAGAGATCTGGATATGCAGGAGGAGATGATGAGTTATCAGCCACAANCCGCGGCCCNNCCCAGCCGTTTTCTTGATGTANAAGnngnngngacnt                                                                                 >  2:108328/1‑185 (MQ=255)
                                                                                                                                                                          ccGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGATATTACCGCCCAATACCGGAATGGATTGCCGGATATGGATCGTTAAGCGCCAGAACAGAGCACTGAATATGCAGGAGAACATGCTGAGTTCTCAGCcaca                                                                                                                                   >  1:500728/1‑141 (MQ=255)
                                                                                                                                                                               aTTTCTGCGGTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTATAAGAATCTGGTAAAACGCTGCGCATCCATGTGAATGACTGCGGACAAGGCGACGAAACCGTTGTCctgc                           <  1:335484/240‑1 (MQ=255)
                                                                                                                                                                                            ncAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAAcc                                     >  1:56223/2‑217 (MQ=255)
                                                                                                                                                                                             cAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTAc  >  1:428303/1‑251 (MQ=255)
                                                                                                                                                                                                cTNNNNNAGCNAAGGGCGTTATTACCGCCCAATCCCGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGtttg                                                                                                          <  2:317795/144‑2 (MQ=255)
                                                                                                                                                                                                                                  ccGGAATGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACan                                                                                                                                                                             <  1:93899/43‑2 (MQ=255)
                                                                                                                                                                                                                                       |                                                                                                                                                                                                                
TGTTGAGGATCAGAGAACGCTGCATCCGCTCAACCCGATTTGGGATAACCAGTTCATGACTTTGCTGGAGCAGGGACGCATACAGGAACTGGATGCCGTCAGTAACGAAGAGCTTTCCGCCATTGCCGGAAAGTCGACACATGAAATCAAAACCTGGGTCGCCGCTTTTGCCGCTATTTCTGCGTTTGGCAACTGGCGTAGCGAAGGGCGTTATTACCGCCCAATCCCGGAGTGGATTGCCGGATTTGGCTCGTTAAGCGCCAGAACAGAGAACTGAATATGCAGGAGAAGATGATGAGTTATCAGCCACAAACCGAAGCCGCCACCAGCCGTTTTCTGAATGTAGAAGAAGCGGGTAAAACGCTGCGCATCCATTTTAATGACTGCGGACAAGGCGACGAAACCGTTGTCCTGCTGCATGGTTCCGGCCCGGGTGCTAC  >  NC_000913/370945‑371384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑