Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,636,217	G→A	A71T (GCG→ACG)	creB →	DNA‑binding transcriptional regulator CreB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,636,217	0	G	A	100.0%	53.7 / NA	20	A71T (GCG→ACG)	creB	DNA‑binding transcriptional regulator CreB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

CTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCA  >  NC_000913/4635972‑4636440                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                cTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCCACGGGAAACGGTCTGGTTCGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTc                                                                                                                                                                                                                            >  1:420819/1‑251 (MQ=255)                                                              ggAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGCGGTCTTTGAGCGAGGCTTGCCGGTGCTGGCTAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTg                                                                                                                                                               >  1:490144/1‑251 (MQ=255)                                                                       gCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCACCAGTTACTGACGCTCCa                                                                                                                                                                                                                          >  1:151611/1‑183 (MQ=255)                                                                                               cTACATGTTGCAGCAGGAAGGTTTTACCGTCGAGGGCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTgtgatgacccgctacaggtgct                                                                                                                              >  1:388808/1‑230 (MQ=255)                                                                                                       tGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCAGACGTCATGATTCTCGATGTTGGTCTGCCGGATCTTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCg                                                                                                                                                                                                                   >  1:480390/1‑158 (MQ=255)                                                                                                         cagcagGAAGGTTTTGCCGTAGCGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTga                                                                                                                                            >  1:421273/1‑227 (MQ=255)                                                                                                                   ggtttGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGTCGTCATGATTCTCGATGTGGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGc                                                                                                                                                                                                                    <  1:423793/143‑1 (MQ=255)                                                                                                                         ccGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGCAGTGTgc                                                                                                    >  1:420020/1‑251 (MQ=255)                                                                                                                                           gAGGCTTGNCNNNGNNGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGNTGGTCTGCCGGATATNAGNGGCTNTGANTTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAACTTGGTGCTgacn                                                                                                                                          >  2:351056/1‑194 (MQ=255)                                                                                                                                           gAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTgacg                                                                                                                                          <  1:351056/195‑1 (MQ=255)                                                                                                                                                         cTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCCGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCggg                                                                        >  1:269586/1‑247 (MQ=255)                                                                                                                                                           ggATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGGCGGATATTAGCGGCGTTGAATTGGGCCGCCAGTTGCTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGAc                                                                                                                                                                                               <  1:455788/126‑1 (MQ=255)                                                                                                                                                                             ggTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGaan                                                                                                                                                                                 <  1:405969/122‑2 (MQ=255)                                                                                                                                                                                                      gTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGAAGCCACATCCGGCGTTAACTGTACTGTTACTGACGACCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCACGCGAAGTGTGCGCCAGGGAGCGCACCTTACTGCGTCGGGGGAAGAAGTTCTCGACGCCCTCTCCCGTCATCCGTATTGGACATTttg                        >  1:465569/1‑250 (MQ=255)                                                                                                                                                                                                                   atatTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTgaagaan                                                                <  1:307038/197‑2 (MQ=255)                                                                                                                                                                                                                   atatTAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCNAAANNNnnttnn                                                                                                                   >  2:307038/1‑144 (MQ=255)                                                                                                                                                                                                                       tAGCGGCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTggn                                                                                                                                                             <  1:315274/100‑2 (MQ=255)                                                                                                                                                                                                                           ggCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTCCCTGTTCTGTTCCTGACGTCCCGAAGTGACGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACTTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCa  <  1:21987/251‑1 (MQ=255)                                                                                                                                                                                                                           ggCTTTGAATTGTGCCGCCAGTTACTGACGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCGTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTccc                                              <  1:371896/207‑1 (MQ=255)                                                                                                                                                                                                                           ggCTTNNAANNNTGNNGCCAGTTACTGACGCTCCATCCGNCGTTACCTGTACTGTTCCTGACGGCCCGANGNGNAGAGNNCGANCGCCTGCTTGNGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGNGNAGTGTGCGCCAGGGTGCGCACCTTACTGCGTNGGGTGAAGAAGTTCTCGACGCCGTNTcccnnatngnnnnnnn                                 >  2:371896/1‑207 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                CTGTCATGCCGTGGCGGCAATAACAGAGGCGATTTATGCAACGGGAAACGGTCTGGTTAGTGGAAGATGAGCAAGGGATAGCCGACACGCTGGTCTACATGTTGCAGCAGGAAGGTTTTGCCGTCGAGGTCTTTGAGCGAGGCTTGCCGGTGCTGGATAAAGCTCGCAAGCAGGTACCCGACGTCATGATTCTCGATGTTGGTCTGCCGGATATTAGCGGCTTTGAATTGTGCCGCCAGTTACTGGCGCTCCATCCGGCGTTACCTGTACTGTTCCTGACGGCCCGAAGTGAAGAGGTCGATCGCCTGCTTGGGCTGGAAATTGGTGCTGACGACTACGTGGCTAAACCGTTTTCACCCCGCGAAGTGTGCGCCAGGGTGCGCACCTTACTGCGTCGGGTGAAGAAGTTCTCGACGCCGTCTCCCGTCATCCGTATTGGACATTTTGAATTGAATGAACCCGCGGCGCA  >  NC_000913/4635972‑4636440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑