| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 914,713 | C→T | A49T (GCG→ACG) | lysO ← | L‑lysine/thialysine exporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 914,713 | 0 | C | T | 96.0% | 62.9 / ‑5.8 | 25 | A49T (GCG→ACG) | lysO | L‑lysine/thialysine exporter |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (9/15); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01 | |||||||||||
GAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGTTTTAGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGCCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGG > NC_000913/914474‑914921 | gAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGTTTTAGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGAGGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATAGTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATAc < 1:410070/251‑1 (MQ=255) aaaGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGTTTTAGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATAGTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATAcc < 1:469839/251‑1 (MQ=255) gcACAGTTTTAGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGCTGGTGGTTGCGCCACGGCAGGCATCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATACAGGTAATACTGACGGCCGACTAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGCGAAACGTCAGACTGCTACCCATAAAAAAGAGAATAAGGTACACCATCCAGCTTAATAGCTGATta > 1:508364/1‑250 (MQ=255) cTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGATGATGGAGGTTGCGCCACGGCAGGCCTCGCTCCCGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCCGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGc > 1:373601/1‑249 (MQ=255) ccATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGAGGCACTATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTgg > 1:435659/1‑251 (MQ=255) tGCTGATGGTGGTTGCGCCCCGGCAGGCTTTGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGCTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGCCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATc < 1:41722/180‑1 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTg < 1:70029/144‑1 (MQ=255) tGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAggg < 1:33408/250‑1 (MQ=255) gATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGGTACTGGCGAGGTTATCGAGAAACGTTAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATGAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACACTCAgggg < 1:261534/250‑1 (MQ=255) gATGGTGGGTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACCCAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCTCCAACAGGTTACTGGCGAGGGTATCGAGAAACGTCAGACTGAGACCCATAAAAAAGAGAATAATGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAGTCAggg < 1:75800/249‑1 (MQ=255) ggtTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATCACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAAcc > 1:275973/1‑248 (MQ=255) ggtTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAAGATTACACAGTAAAATATAGGTAAGACTGACGGCAGAATAATACAGAATCGCCAACAGGTTCCTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTACCCCACAATCAGGGGAAcc > 1:443741/1‑248 (MQ=255) ccACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACGGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAATCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTa < 1:193418/251‑1 (MQ=255) cACGGCAGGCCTCGCGCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCATTCGGATTCCCAGAAAAAAGAGAATAAGTTAAACCGGCCAGCTTAATAGCTGATTAATAGCTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAgg < 1:463964/234‑1 (MQ=255) cAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATtaata > 1:204737/1‑166 (MQ=255) aaTACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGa < 1:187507/250‑1 (MQ=255) ataatGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAAGCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCg < 1:7596/251‑1 (MQ=255) nccAACAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAAt > 1:313384/2‑158 (MQ=255) ccAACAGGTTACTGGCGAGGTTATCGAGAAACGTCCGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTACTAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATg > 1:414234/1‑202 (MQ=255) aaCAGGTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGAtn < 1:105442/218‑2 (MQ=255) gTTACTGGCGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAgn < 1:146398/79‑2 (MQ=255) nnggNNNNNTTANCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAAt < 2:313384/144‑1 (MQ=255) cGAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAAccn < 1:131364/122‑2 (MQ=255) gAGGTTATCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAgg > 1:231576/1‑226 (MQ=255) aTCGAGAAACGTCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCTGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCt < 1:230988/170‑1 (MQ=255) | GAAAGCCAGTCCACTTAGACCAATGGCAAAACCAATCACTACTACGCCGCACAGTTTTAGCGACTCCAGCGCCATCGCAATACGCGACGGGAGTTTTTCTTGCTGATGGTGGTTGCGCCACGGCAGGCCTCGCTCCAGCCACATCAGGGCGGCAATATTACACAGTAAAATAACGGTAATACTGACGGCAGAATAATGCAGAATCGCCAACAGGTTACTGGCGAGGTTATCGAGAAACGCCAGACTGATACCCATAAAAAAGAGAATAAGGTAAACCATCCAGCTTAATAGCTGATTAATAACTTTTAACGCAGCTTGTTGGCGAAGCGGAATGAGGTAACCCACAATCAGGGGAACCAGAATGATTAACAGCCCAGAAAACATGAAAACCCAGTCCTTGCAAAGATGAAGTCGAAATGCGCGATGACACACTACTGAAAGCGGAAGG > NC_000913/914474‑914921 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |