Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	510,530	G→A	L284F (CTT→TTT)	copA ←	Cu(+) exporting P‑type ATPase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	510,530	0	G	A	100.0%	39.6 / NA	15	L284F (CTT→TTT)	copA	Cu(+) exporting P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/9);  total (6/9)

GATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGGCCGCCGGCGAAAACCATCACTGCCAGGGTTATCAGGCCGATAACCAACCACAGGCTGCGGTTGTCAGCGGTGACCATCATG  >  NC_000913/510320‑510756                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   gATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTAAGTAACCAGGCGTGCCGTCGGCGGGGTTACATCGAGCAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGACTATGGCCGAGATTGATCCGACCGGTAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGa                                                                                                                                                                                                                >  1:256140/1‑231 (MQ=255)            gtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCCGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTGCGGc                                                                                                                                                                                                     >  1:430519/1‑231 (MQ=255)                              ncTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGCGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGAAGCTCCTCGAATAGAGCCacgccacgcc                                                                                                                                                               >  1:91402/2‑251 (MQ=255)                                                                                  ggCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGc                                                                                                                                                                                        <  1:441433/174‑1 (MQ=255)                                                                                              ggggggTTAAATCGAGTAACTTTTACAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGATTCCTGAATATGGCCGAGAATGATCAGACCGATAATAATCGCGCTGGATTCGTAATAAAAATGTCGCGCTTCCATAGGGAACCACTGCGGCAACAGGTTTACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGAGAATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTg                                                                                               >  1:462439/3‑251 (MQ=255)                                                                                                                   tttCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATGGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAAGAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGGCCGCCgg                                                                            <  1:452193/249‑1 (MQ=255)                                                                                                                          cgcCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTgn                                                                                                                                                                                                        <  1:148942/118‑2 (MQ=255)                                                                                                                               tAGAAGAACTGTGGCGTGCGCGCGCTTCCAGCAGATGGCCGATATTGATCAGACCGATAATCATCGCGCTGGCTTCGGAATAAAAATGTCGCGCTTCCATCGGGACCCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCGCCGTGCAGCATGCTTTTCCATGCACTGCGGTAAAAATGGCCGCCGGCGAAAACCATCAc                                                               <  1:353589/250‑1 (MQ=255)                                                                                                                                              gTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTc                                                                                                                                                                                                                               >  1:190178/1‑75 (MQ=255)                                                                                                                                                         tCNAGCATNTGGCNGAGATTGATCAGACCNATAATCATCGCGCTGGCTTNNTNATAAANATGTc                                                                                                                                                                                                                               <  2:190178/64‑1 (MQ=255)                                                                                                                                                                 atGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACGGCGGCCACAGGTTGACGCACATCGAAAAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGGCCGCCGGCGAAAACCATCACTGCCAGGGTTATCAGGCCGATAACCAACCACAGGc                            <  1:372345/251‑1 (MQ=255)                                                                                                                                                                                          atcatcGCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTGCGGc                                                                                                                                                                                                     <  1:143035/57‑1 (MQ=255)                                                                                                                                                                                            catcTCGCTGGCTTCGTAATAAAAATGTCGCGCTTCCAGCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCTTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGGCCGCCGGCGAAAACCATCACTGCCAGGGTTAGCAGGCCGATAAGCAACCACAGGCTGCGGTTGTCAGCGGTGACCATCATg  <  1:323928/250‑1 (MQ=255)                                                                                                                                                                                            catcGCGCTGGCTTCGTAATAAAAATGTCGCGTTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGGccgccgn                                                                            <  1:103938/176‑2 (MQ=255)                                                                                                                                                                                               cgcgCTGGCTTCGTAATAAAAATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATgg                                                                                   >  1:414265/1‑166 (MQ=255)                                                                                                                                                                                                           gTAATAAAAATTTCGCGCTTCCATCGGGAACCACGGCGGCCACAGGTTGCCGCTCATCGAATAGAGCCACTCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTGCAGCAGGCTTTTCCATGCACTGCgn                                                                                            <  1:101635/145‑2 (MQ=255)                                                                                                                                                                                                                   |                                                                                                                                                                                                                                   GATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCGCGCGCTTCCAGCATATGGCCGAGATTGATCAGACCGATAATCATCGCGCTGGCTTCGTAATAAAGATGTCGCGCTTCCATCGGGAACCACTGCGGCCACAGGTTGACGCTCATCGAATAGAGCCACGCCACGCCAGTACCCAGCGCCACCAGCGTATCCATCGTCGCCGCACCGTTCAGCAGGCTTTTCCATGCACTGCGGTAAAAATGGCCGCCGGCGAAAACCATCACTGCCAGGGTTATCAGGCCGATAACCAACCACAGGCTGCGGTTGTCAGCGGTGACCATCATG  >  NC_000913/510320‑510756

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑