Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,979,089 C→T Y163Y (TAC→TAT)  wzyE → enterobacterial common antigen polymerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,979,0890CT100.0% 59.0 / NA 22Y163Y (TAC→TAT) wzyEenterobacterial common antigen polymerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

CCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTACTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGACTTATATGATTGTCGGCGGCACTCGCGCCAATATCATCATCGCATTCGCTATCTTCCTGTTTATTGGCATTATTCGCGGCTGGATTTCGTTG  >  NC_000913/3978863‑3979278
                                                                                                                                                                                                                                  |                                                                                                                                                                                             
ccTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTc                                                                                                                                                                                         >  1:39160/1‑233 (MQ=255)
                 ctccaaCGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTAACATGCACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGGATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACACCGGCTTTTTGCTGTTCCTGCTTCACTCCTCCAGTCATATCTTTTCCAGTGCCGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCTATTCTGGTGGTCTACttagatctga                                                                                                                                                       >  1:363351/5‑242 (MQ=255)
                       cgcgTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCCATCTTACGGTGGTGATCCTGATGGGTAACGCGCTTGTAAGCGTCGGCATCTTCCTCATGCACAACGGCTTGTTGCTGGTCCGGCTTAACTCCTTCCGTTAGATCCTTTCCAGTGAAGGCTCCGGCGTGGCGGTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAg                                                                                                                                                <  1:154714/251‑1 (MQ=255)
                             gCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGAGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCTGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGc                                                                                                                                          >  1:505364/1‑251 (MQ=255)
                                             gTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTcc                                                                                                                            >  1:411936/1‑249 (MQ=255)
                                                            tGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGt                                                                                                                                        <  1:402395/222‑1 (MQ=255)
                                                              aatcgcgTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTGTGCTGTTCCGGCTTAACGCCTACAGTCAGATCTTTTCCAGTGAAGTTTCCAGCTTGGCTTTAAATCGCTTCTTTTATATTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTgg                                                                                                                                      <  1:64616/219‑1 (MQ=255)
                                                                 cgcgTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTgn                                                                                                      <  1:97431/251‑2 (MQ=255)
                                                                  gcgTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTCAGCGCCGGCCTCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTATCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTTATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGt                                                                                                                                        >  1:368022/1‑216 (MQ=255)
                                                                            naaTCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGACtt                                                                                           >  1:91482/2‑251 (MQ=255)
                                                                                         gTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGCCCTTTTGCTGTCACGCCTTAACTCCTACAGTCAGATCTTTTCCAGTGACGTCTCCGGCGTGGCGTTAACCCGCTTCTTTTATTTTTTCATCCTGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCCCGGTCGCCTTTGGCTTGCTGACTTATATGATTGTcgg                                                                              >  1:489921/1‑251 (MQ=255)
                                                                                             tCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGAGGCTGGTGGTCTACTTTCTGCTCCAGGACAGCAAAGc                                                                                                                                          <  1:424874/187‑1 (MQ=255)
                                                                                               cTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTc                                                                                                                                                                    >  1:35561/1‑159 (MQ=255)
                                                                                                          cgcgCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTTACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCg                                                                                                                         <  1:415310/191‑1 (MQ=255)
                                                                                                           gcgcTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTgn                                                                                                 <  1:55416/214‑2 (MQ=255)
                                                                                                                                                         tGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCCTCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGACTTATATGATTGTCGGCGGCACTCGCGCCAATATCATCATCGCATTCGCTATCTTCCTGTTTATTGGCATTATTCGCGGc              >  1:243002/1‑251 (MQ=255)
                                                                                                                                                             ccGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGACGACTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCCTCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGACTTATATGATTGTCGGCGGCACTCGCGCCAATATCATCATCGCATTCGCTATCTTCCTGTTTATTGGCATTATTCGCGGCTgg           >  1:185608/1‑250 (MQ=255)
                                                                                                                                                                   tAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCAc                                                                                                                  <  1:461037/141‑1 (MQ=255)
                                                                                                                                                                        ccTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGGTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTGCCTCGGCAGCACGGTCGCCTTTGGCTTGCTGACGTATATGATTGTCGGCGGCACTCGCGCCAATATCATCATCGCATTCGCTAGCGTCCTGTTTATTGGCATTATGCGCGGCTGGATTTCGTtg  <  1:461499/248‑1 (MQ=255)
                                                                                                                                                                              gTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGAt                                                                                                                                                                              <  1:246150/70‑1 (MQ=255)
                                                                                                                                                                                cAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTTGTGGTCTACTGTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGAc                                                                                             <  1:195474/149‑1 (MQ=255)
                                                                                                                                                                                                                gtTAAAACGCTTCTTTTATTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTg                                                                                               <  1:279423/115‑1 (MQ=255)
                                                                                                                                                                                                                                  |                                                                                                                                                                                             
CCTACAAAACCCGCCTACGCAAACGCGTTGCTGATGTACCGCGCCGTCCGCTGTTTACCATGAACCGCGTGGAGACCAATCTTACGTGGGTGATCCTGATGGGTATCGCGCTGGTAAGCGTCGGCATCTTCTTCATGCACAACGGCTTTTTGCTGTTCCGGCTTAACTCCTACAGTCAGATCTTTTCCAGTGAAGTCTCCGGCGTGGCGTTAAAACGCTTCTTTTACTTTTTCATCCCGGCGATGCTGGTGGTCTACTTTCTGCGCCAGGACAGCAAAGCGTGGCTGTTTTTCCTCGTCAGCACGGTCGCCTTTGGCTTGCTGACTTATATGATTGTCGGCGGCACTCGCGCCAATATCATCATCGCATTCGCTATCTTCCTGTTTATTGGCATTATTCGCGGCTGGATTTCGTTG  >  NC_000913/3978863‑3979278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑