Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,613,740	G→A	R87K (AGG→AAG)	yjjV →	putative metal‑dependent hydrolase YjjV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,613,740	0	G	A	100.0%	38.4 / NA	16	R87K (AGG→AAG)	yjjV	putative metal‑dependent hydrolase YjjV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

GCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGATCTCTTTGGCGACGATCCGCAATTTGAGAGGCAGCAGTGGTTACTCGACGAACAACTGAAACTGGCGAAACGCTACGATCTGCCGGTGATCCTGCATTCACGGCGCACGCACGACAAACTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTT  >  NC_000913/4613536‑4613986                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       ncGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTggg                                                                                                                                                                                                                             >  1:150337/2‑232 (MQ=255)             ccAGCCTGCAACGAGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGATCTCTTTGGCGACGAt                                                                                                                                                                                              >  1:397900/1‑251 (MQ=255)                 ccTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCTCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGATCTCTTTGGCGACGATCCGc                                                                                                                                                                                          <  1:86778/251‑1 (MQ=255)                          cgcgGCACAAGCGGCCGTAGGCAAGATCCTTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGATCTCTTTGGCGACGATcc                                                                                                                                                                                            >  1:112468/1‑240 (MQ=255)                              gCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTTCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCGCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGtggtgg                                                                                                                                                                                                                                    <  1:371973/196‑1 (MQ=255)                               cacaAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGATCTCTTTGGCGACGATCCGCAATTTgag                                                                                                                                                                                  <  1:229683/245‑1 (MQ=255)                                 caAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTTGTGGCGGTTGGTGAGATCGGTCTGGATCTCTTTGGc                                                                                                                                                                                                    <  1:153052/225‑1 (MQ=255)                                                                          ggAAAATTTTGCCCTTGTGTTGGCATTAGCGGAAAATTATCAACCGCTTTATGCCGCATTGGGCTTGCATCCGGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACACCAGGCGCTGGAAAAGCGTCCGGCGa                                                                                                                                                                                                                                             <  1:186870/143‑1 (MQ=255)                                                                               aTTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATGGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACATCAGGCGCTGGAAAAGCGTcc                                                                                                                                                                                                                                                  <  1:182928/133‑1 (MQ=255)                                                                                                gCATTAGCGGAAAATTATCAAACGCTGTATGCAGCATTGGGCTTGCATACTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATc                                                                                                                                                                                                                      >  1:417398/1‑144 (MQ=255)                                                                                                                   aaCCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTgggg                                                                                                                                                                                                                            >  1:28929/1‑119 (MQ=255)                                                                                                                             tGCCGCATTGGGCTTGCATCCTGGGATGTTGAAAAAACATAGCGATGTGTCTCTTGAGCAGCTTCAGAAGGCGCTGGAAAAGCGTCCGGCGACGGTGGTGGCGGTGGGGGAGATc                                                                                                                                                                                                                      >  1:335928/1‑115 (MQ=255)                                                                                                                                                   gngaTGTTGGAAAAACATAGCGANNTGTCGCTTGNGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGNGAGCGGTCTNNAANTCTTGNGNNNCNNNCCNCAATTTGATAGGCAGCAGTNGTNNCTCGACNAACAACTGAAACTGGCGAAACGCTAc                                                                                                                                  <  2:111106/174‑1 (MQ=255)                                                                                                                                                   ggTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGGCTGGAACTCTTTGGCGACGATCCGCAATTTGAGAGGCAGCAGTGGTTACTCGACGAACAACTGAAACTGGCGAAACGCTAc                                                                                                                                  >  1:111106/1‑177 (MQ=255)                                                                                                                                                                                                   gcgcTGGAAAAGCGTCCGGCGAAGGTGGTGGCGGTGGGGGCGATCGGTCTGGATCTCTTTGGCGACGATCCGCAATTTGAGCGGCAGCAGTGGTTACtaga                                                                                                                                                              >  1:464858/1‑98 (MQ=255)                                                                                                                                                                                                         gAAAAGCGTCCGGCGTAGGTGGTTGCGGTGGGGGAGAGCGGTCTGGATCTCTTGGGCGACGATCCGCAATTTGAGAGGCAGCAGTGGTTACTCGACGAACAACTGAAACTGGCGAAACGCTACGATCTGCTGGTGATGCTGCAGTCACGGCGCACGCACTACAAACTGGCGATGCATGTTAAACGCCACGAGTTACCGCTCGCTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGATCGGtt  <  1:294477/251‑1 (MQ=255)                                                                                                                                                                                                             |                                                                                                                                                                                                                                                       GCGATGAAGAGGCCAGCCTGCAACGCGCGGCACAAGCGGGCGTAGGCAAGATCATTGTTCCGGCAACAGAGGCGGAAAATTTTGCCCGTGTGTTGGCATTAGCGGAAAATTATCAACCGCTGTATGCCGCATTGGGCTTGCATCCTGGTATGTTGGAAAAACATAGCGATGTGTCTCTTGAGCAGCTACAGCAGGCGCTGGAAAGGCGTCCGGCGAAGGTGGTGGCGGTGGGGGAGATCGGTCTGGATCTCTTTGGCGACGATCCGCAATTTGAGAGGCAGCAGTGGTTACTCGACGAACAACTGAAACTGGCGAAACGCTACGATCTGCCGGTGATCCTGCATTCACGGCGCACGCACGACAAACTGGCGATGCATCTTAAACGCCACGATTTACCGCGCACTGGCGTGGTTCACGGTTTTTCCGGCAGCCTGCAACAGGCCGAACGGTT  >  NC_000913/4613536‑4613986

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑