| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,159,493 | C→T | G433D (GGC→GAC) | sthA ← | soluble pyridine nucleotide transhydrogenase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,159,493 | 0 | C | T | 92.9% | 29.3 / NA | 15 | G433D (GGC→GAC) | sthA | soluble pyridine nucleotide transhydrogenase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/8); minor base G (0/1); total (6/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCTGGCAGAGGCCATCCGCGCAAGAATGGATGGCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGAAGTACTCAATAGTGTTGCCGCCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCGTGCCCACGTTCATGCCGACGATTTGTGCGCGTGCCAGATGTTTAAACTGGGCGCGGCCCACTTCATATGGCACTTTCATTGCGGTCAGCTGCTGTTCGGTTTTGCCCACAGAGCTGATT > NC_000913/4159275‑4159726 | ggAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGCGCAGCTGGCAGAGGCCATCCGCGCAAGAATGGATGGCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTACCCCATCGTCGGGGCGTTAAAGGCGGTGTTGACGAAGTAATCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGat > 1:253147/1‑250 (MQ=255) cACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCTGGCAGAGGCCATCCGCGCAAGAATGGATGGCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGAAGTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCg < 1:344624/251‑1 (MQ=255) cTCACCGCTGCGCAGCCGCTATGAGCAGCTGGCAGAGGCCATCCGCGCAAGAATGGATGGCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGGCGGGTAGTTAAAGGTGGTGTTGACGAAGTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAgc < 1:331027/229‑1 (MQ=255) ggCAGAGGCCATCCGCGCAAGAATGGATGGCAATTTCGATATAGTTTTACAACAGGCGGTTTAAACCGTTTACAGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTCGTTAAAGGTGGTGTTGACGAAGTACTCCCTAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCg > 1:328409/1‑206 (MQ=255) gCCATCCGCGCAAGAATGGATGGCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGAAGTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAAGCGCCTGAcc < 1:119699/171‑1 (MQ=255) ggCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGAAGGACGCAATAGTGTTTGCGGCTCCTTTCGGTGCCATAATCGCCGGACCGGTATGAATAATTTCTGCAGCGCGCTCGGCAAAGCAGTGAAGACTCAGATTCTCTTTTGTTGCCCGATGGAACAAAATTTTCAGCGTGCCCACGTTCATGc < 1:276311/251‑1 (MQ=255) gTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGGTAAAGGTGGTGTTGACGAAGTACACAATAGTGTTGCCGACACCGTTCTTTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCATTGATTACCCCGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCGTGc > 1:25968/1‑200 (MQ=255) cGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGCAGTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATACTCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCg > 1:79865/1‑133 (MQ=255) ncTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGAAGTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCGTGCCCACGTTCATGCCGACGATTTGTGCGCGTGCCAGATGTTTAAACTGGGCGCGGCCCACTTc > 1:315040/2‑251 (MQ=255) aCCCGATAGGCTTCAGCCATCGTCGGGTAGTTAAAGGTGGTGTGGACGAAGTACTCAAGAGTGTTGCCGTCACCTTTc < 1:456520/78‑1 (MQ=255) nngAANTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCTATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCg < 2:317280/137‑1 (MQ=255) aGTACTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCGTGCCCACGTTCATGCCGACGATTTGTGCGCGTGCCAGATGTTTAAACTGGGCGCGGCCCACTTCATATGGCACTTTCATTGCGGTCAGCTGCTGTTCGGTTTTGCCCACAGAGCTg < 1:23255/251‑1 (MQ=255) aCTCAATAGTGTTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCGTGCCCACGTTCATGCCGACGATTTGTGCGCGGGCCAGATGTTTAAACTGGGCGCGGCCCACTTCATATGGCACTTTCTTTGCGGTCAGCTGCTGTTCGGTTTTGCCCACAGAGCTGAtt > 1:110092/1‑251 (MQ=255) tCAATAGTGTTGCCGTCTCCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTccc < 1:507352/109‑1 (MQ=255) tgtTGCCGTCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCATAAtctc < 1:108500/91‑1 (MQ=255) | GGAACCACGCCGCACTATTGGCCTGGTTTATCGTCCTGGCTCACCGCTGCGCAGCCGCTATGAGCAGCTGGCAGAGGCCATCCGCGCAAGAATGGATGGCCATTTCGATAAAGTTTTAAAACAGGCGGTTTAAACCGTTTAACGCAGCTACCCGATAGGCTTCCGCCATCGTCGGGTAGTTAAAGGTGGTGTTGACGAAGTACTCAATAGTGTTGCCGCCACCTTTCTGTTCCATAATCGCCTGACCGATATGAATAATTTCGGCAGCGCGCTCGCCAAAGCAGTGAATACCCAGAATCTCTTTTGTTTCCCGATGGAACAAAATTTTCAGCGTGCCCACGTTCATGCCGACGATTTGTGCGCGTGCCAGATGTTTAAACTGGGCGCGGCCCACTTCATATGGCACTTTCATTGCGGTCAGCTGCTGTTCGGTTTTGCCCACAGAGCTGATT > NC_000913/4159275‑4159726 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |