Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,380,629	G→A	G161D (GGC→GAC)	ycjU →	beta‑phosphoglucomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,380,629	0	G	A	100.0%	40.3 / NA	16	G161D (GGC→GAC)	ycjU	beta‑phosphoglucomutase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

AACGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCAGATCTCCGTGCACAGCAGATCTCGGTTGGGCTGGCTTCTGTCTCCCTGAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGGCGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCAC  >  NC_000913/1380421‑1380766                                                                                                                                                                                                                 |                                                                                                                                          naCGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCAGATCTCCGTGCACAGCAGATCTCGGTTGGGCTGGCTTCTGTCTCCCTGAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGAAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGCAGATGCGCAggg                                                                                                 >  1:90592/2‑250 (MQ=255)              ccGGCATTCGCTCTTTGCTGGCAGATCTCCGTGCACAGCATATCTCGGTTGGGCTGGCTTCTGTCTCCCTGAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTgc                                                                                                                                      <  1:38547/201‑1 (MQ=255)                                                                                cccTGAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTg                  <  1:289331/251‑1 (MQ=255)                                                                                    gAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGcc                <  1:142413/249‑1 (MQ=255)                                                                                                 aTTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGAcn                                                                                      <  1:311811/166‑2 (MQ=255)                                                                                                 aTTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTANCGGNNNNNaannnnnn                                                                                                      >  2:311811/1‑144 (MQ=255)                                                                                                            gcTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAgg                                                                                                  >  1:440750/1‑143 (MQ=255)                                                                                                                     ncgcgAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACg                                                                                      >  1:128045/2‑146 (MQ=255)                                                                                                                                              naTGCTTCCCAACTTAAAAACTCGAACCCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGCCTGGACGTGCCGCTGCAGGCATGTATCGGCATTGAAGAGGCGCAGGCGGGCATTGCCGCCATTAACGcc                                                                           >  1:100606/2‑132 (MQ=255)                                                                                                                                                tGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGCGCCTCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGc                 <  1:365432/188‑1 (MQ=255)                                                                                                                                                  cTTCCCAACGTAAAAACTCGAAACCGGACCCGTAAAGCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGTGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGgcg                                               <  1:361894/156‑1 (MQ=255)                                                                                                                                                          cTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCAGTGAAGATGCGCAGGCGGGCATTGACGCCATGAACGCCAGCGGTATGCAGTCGGTggn                                                       <  1:149914/140‑2 (MQ=255)                                                                                                                                                          cTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGACGCCGCCGGCATGTATCGGCATTGAAGATGCTCAGGCGGGCTTTGACGCCATTACCCCCCGCGGGCTGCGCTCAGGGGGGATCGGCGCGGGCTTAACCGGGGCGCCATTACTGTTGCCTTCAACGGAAtccc  >  1:351706/1‑191 (MQ=255)                                                                                                                                                          cTTAAAAACTCGAAACCGGACCCGGAAAGCTTTCTCGCCGCCTGGGCGGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATNGACNCCATNAANGCCAGCGGTATGCGCNCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGNNTNNNANGTAAtcac  <  2:351706/193‑1 (MQ=255)                                                                                                                                                                  ntCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGACGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGtt                   >  1:125440/2‑168 (MQ=255)                                                                                                                                                                     gAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAACGCAGGACGTGCCGCAGCAGGCATGTCTCGGCATTg                                                                                                              >  1:477411/1‑74 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                          AACGCTGTTCTACCCGGCATTCGCTCTTTGCTGGCAGATCTCCGTGCACAGCAGATCTCGGTTGGGCTGGCTTCTGTCTCCCTGAATGCGCCGACGATTTTAGCGGCGCTGGAGCTGCGCGAGTTTTTCACCTTCTGCGCGGATGCTTCCCAACTTAAAAACTCGAAACCGGACCCGGAAATCTTTCTCGCCGCCTGTGCAGGGCTGGGCGTGCCGCCGCAGGCATGTATCGGCATTGAAGATGCGCAGGCGGGCATTGACGCCATTAACGCCAGCGGTATGCGCTCGGTGGGGATCGGCGCGGGCTTAACCGGGGCGCAATTACTGTTGCCTTCAACGGAATCAC  >  NC_000913/1380421‑1380766

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑