Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,608,089	C→T	A248A (GCC→GCT)	tam →	trans‑aconitate 2‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,608,089	0	C	T	100.0%	39.9 / NA	16	A248A (GCC→GCT)	tam	trans‑aconitate 2‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

AGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCCCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAACAGGTCAATTTCAATAGCGTTGCAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGT  >  NC_000913/1607902‑1608319                                                                                                                                                                                            |                                                                                                                                                                                                                                       aGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGTAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTTCGGGCATTTCCGCGTCTGTGTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCg                                                                                                                                                                                             <  1:270825/231‑1 (MQ=255)                   cGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACATGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCt                                                                                                                                                                  <  1:450264/240‑1 (MQ=255)                                  tGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGtt                                                                                                                                                          <  1:472455/233‑1 (MQ=255)                                       gCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGt                                                                                                                                                                                                                                  <  1:257167/156‑1 (MQ=255)                                                                       naCCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTCTCCACTGCAAGAGGCCGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGtcagctgacgtg                                                                                                                                                                                             >  1:90056/2‑150 (MQ=255)                                                                             gagCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAata                                                                                                                                                                                                                      >  1:196093/1‑130 (MQ=255)                                                                               ncGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCCTTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAACAGGTCAATTTCAATAGCGTTGCAGATGGCATcc                                                                                                               >  1:96072/2‑231 (MQ=255)                                                                                          cTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAANGACNNNNnggnn                                                                                                                                                                                         >  2:298759/1‑144 (MQ=255)                                                                                                  tAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAACAgg                                                                                                                                             <  1:171591/182‑1 (MQ=255)                                                                                                        cTACCATCAGATGCTGGAAGAGAAGTATGCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGNNTATGGANNa                                                                                                                                                                                                                          >  2:3890/1‑99 (MQ=255)                                                                                                        cTACCATCAGAGGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGATAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGTCTTCATCGTTCATCTGTAACAgcg                                                                                                                                            <  1:3890/177‑3 (MQ=255)                                                                                                            cATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGAATTTCAGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAACAGGTCAATTTCAATAGCGTTGCAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCg                                                              >  1:445626/1‑251 (MQ=255)                                                                                                              tCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCtn                                                                                                                                                                                                          <  1:63897/109‑2 (MQ=255)                                                                                                                                             gngaNCGGACNGATANTGCTGGCATTTCCGCNTCTGTTTAGTGTTGCTCGGNNTNTGGAGNAAata                                                                                                                                                                                                                      <  2:196093/66‑1 (MQ=255)                                                                                                                                                 aCGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTc                                                                                                                                                                      >  1:2879/1‑110 (MQ=255)                                                                                                                                                                         ctcgTCTGTTTATTGTTGCTCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAACAGGTCAATTTCAATAGCGTTGCAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGt  >  1:461954/3‑250 (MQ=255)                                                                                                                                                                                            |                                                                                                                                                                                                                                       AGATGCCGTCACACCAGGCGATTATCGATTGGGTGACTGCCACTGGATTACGTCCGTGGTTACAGGATCTGACCGAGAGCGAACAGCAGCTTTTTCTTAAGCGCTACCATCAGATGCTGGAAGAGCAGTATCCACTGCAAGAGAACGGACAGATACTGCTGGCATTTCCGCGTCTGTTTATTGTTGCCCGGCGTATGGAGTAAATATCACGTCAGCTGGTAATGACGATCGGGAAGAATTTTTGCTGGAATTTCGGCTTCATCGTTCATCTGTAACAGGTCAATTTCAATAGCGTTGCAGATGGCATCCAGTGGTAAATCATTGTTTTCAGTACCGAACGGATCTTCCAGTTCTTCCGCCAGACAATCCAGCGAAATAAAAGTGTAGGAAATCAGCACAGAGATAAAAGGCGTCATGT  >  NC_000913/1607902‑1608319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑