Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,241,019 T→A F359Y (TTC→TAC)  sstT → serine/threonine:Na(+) symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,241,0190TA94.7% 43.6 / NA 20F359Y (TTC→TAC) sstTserine/threonine:Na(+) symporter
Reads supporting (aligned to +/- strand):  ref base T (1/0);  major base A (7/11);  minor base G (0/1);  total (8/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTC  >  NC_000913/3240827‑3241256
                                                                                                                                                                                                |                                                                                                                                                                                                                                              
gAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCgg                                                                                                                                                                                         <  1:452399/247‑1 (MQ=255)
                         agcaATGACTATTACCGTGGTGACGCTGGCTGCGGTTAATACGCTTGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGGGCCTGTGGCGCATCCGGCGTGGCCGGTGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACTG‑TATCTCGAACGATATCGCCAGGCAGTTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCgcgc                                                                                                                                                            <  1:239895/251‑1 (MQ=255)
                          gcaATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGGAGGTGGTTGCCGTCGGCTTTa                                                                                                                                                                                                >  1:410191/1‑214 (MQ=255)
                               cACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGGTCCCCCTGGCCTGTAATATGTACGG‑TATTTCGAAc                                                                                                                                                                                                                                  >  1:143764/1‑175 (MQ=255)
                                           gtTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCGGTGTGCCTGTGGCGCATCCTGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTTGTTGCCGGCGGCTTTAGCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGAc                                                                                                                                           <  1:277506/250‑1 (MQ=255)
                                              gACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGTGTGGGTCTCTGCTTCTGATCTCACTGGCCGGTAATATGTACGG‑TATGTCGAACGATATCGCCATGCAGGTGGTTGCCGGCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCTAAACCGCGCTGAACTCTTCAACTGa                                                                                                                                            <  1:270610/246‑1 (MQ=255)
                                                      ntgCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATACAGGGGGTTGCCGTCGGCTTTATCATCGGCGGATTTCCGGACTCTTGCGGAAACGCGCTGAACTCTTCCACTGACGTGCTGGTCACt                                                                                                                               >  1:311890/2‑251 (MQ=255)
                                                              aaTACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGTCTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGc                                                                                                                       <  1:357596/251‑1 (MQ=255)
                                                                                   naTCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGCACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAAc                                                                                                                                                       >  1:57730/2‑198 (MQ=255)
                                                                                          ccACGGCGCTGATGTTGAGCGCGGTGGCTTCTCTGTGTGCCTGTGGCGCGTCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTTGCCCGTAATATGTTCGGGAATTTC‑CACGATATCGCCATGGCGGGGGTTTCCGTCGGCTTTCTCATCGGCGTTTTTCCGGACTCTTTCGAAACCGCGCCGAACTCTTCAACCGACGTGTTGTTCACTGCGGCAGCTTGCCCGGCAGAAGTCGATCGTCTGGcc                                                                                           >  1:136209/1‑250 (MQ=255)
                                                                                                  ctgctgTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGc                                                                                                                                                                                                    <  1:33867/138‑1 (MQ=255)
                                                                                                                 gtggCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCggn                                                                                                                          <  1:300699/197‑2 (MQ=255)
                                                                                                                                              cGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAAGAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAgn                                       <  1:102511/251‑2 (MQ=255)
                                                                                                                                                                    ctgctgATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTgg                                                                                             >  1:261441/1‑175 (MQ=255)
                                                                                                                                                                      gctgATCCCACTGGCCTGTAATATGTACGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAAGTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATc               <  1:222593/251‑1 (MQ=255)
                                                                                                                                                                        nnnnnnnnnnggNNNNNAATNTGTACGG‑TATTTCGAACGATATCGCCATACAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGc                                                                                                              <  2:311890/144‑1 (MQ=255)
                                                                                                                                                                             ccACTGGCCTGTAATATGTACGG‑TATTTCGAACGATCTCGCCATGCAGGTGGTTGCCGTCGGCTTTCTCATCGGCGTATTGCCGGACTCATGCg                                                                                                                                                                     >  1:477269/1‑94 (MQ=255)
                                                                                                                                                                                  ggCCTGTACTATGTACGG‑TATTTCGAACGATATCGACCTGCAGGTGGTTGCCGTCGGCTTTCTCCTCGGCGTATTGCAGGACTCTTGCGCAACCGCGCTCAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAgg                                                                                                               >  1:406932/1‑143 (MQ=255)
                                                                                                                                                                                  ggCCTGTAATATGTACGG‑TATTTCGAACTATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCGTCAACTGACGTGCTGTTCACGGCGGCAGCTGGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTa                                                               <  1:232960/191‑1 (MQ=255)
                                                                                                                                                                                   gCCTGTAATATGTGCGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGGTTTCTCAACTTTAAACGGATCAATTCCCCTTTTc  <  1:421632/251‑1 (MQ=255)
                                                                                                                                                                                                |                                                                                                                                                                                                                                              
GAGCCACCATCAATATGGCGGGCGCAGCAATCACTATTACCGTGTTGACGCTGGCTGCGGTTAATACGCTGGGTATTCCGGTCGATCTGCCCACGGCGCTGCTGTTGAGCGTGGTGGCTTCTCTGTGTGCCTGTGGCGCATCCGGCGTGGCGGGGGGGTCTCTGCTGCTGATCCCACTGGCCTGTAATATGTTCGG‑TATTTCGAACGATATCGCCATGCAGGTGGTTGCCGTCGGCTTTATCATCGGCGTATTGCAGGACTCTTGCGAAACCGCGCTGAACTCTTCAACTGACGTGCTGTTCACTGCGGCAGCTTGCCAGGCAGAAGACGATCGTCTGGCAAATAGCGCCCTGCGTAATTAATTGTTTAACCCCTTTCGTCTACGGCGGAAGGGGTTTTCTCAACTTTAAACGGATCAATTCCCCTTTTC  >  NC_000913/3240827‑3241256

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑