| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,609,438 | G→A | E318K (GAA→AAA) | hyfG → | hydrogenase 4 catalytic subunit HyfG |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,609,438 | 0 | G | A | 96.0% | 73.4 / NA | 25 | E318K (GAA→AAA) | hyfG | hydrogenase 4 catalytic subunit HyfG |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/15); minor base C (1/0); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
ATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTA > NC_000913/2609247‑2609679 | ataCCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcgccgc < 1:114871/251‑1 (MQ=255) ccAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGat > 1:154660/1‑251 (MQ=255) aTTCGCACGATTCTGCTGGAAGTCGAACGGCTACACAGTCAGTTGCTTAACCTTGGCCTCTCCTGCGATTTCGTTGGTTTTGATGCCGGCTTGATGCAATTTGTCCGCGTGAGGGAAAAGTCGAGGACTATGGCGAATTTGCTGATGGGGTCGGTTAAGACCTACGGTCTGAATCTGACTGGTGGTGTTCGCCGCGGTATTCGCAAAGAGCAAGGTCTTCAAACGCTGAAACTGGTGCGCGAGATgcgcgc < 1:226588/251‑1 (MQ=255) cgctcgctTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCg > 1:143568/1‑250 (MQ=255) ntgGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGCAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGaa > 1:54605/2‑215 (MQ=255) ngAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTgg > 1:124236/2‑251 (MQ=255) ggAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTgn < 1:405069/251‑2 (MQ=255) aGTCGAACGGCTACACAGTCATTTGCTTAATCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGATTTATGCAATTTTTCCGCGTGCGGGAAAAGGCGATGACGATGGCGAAATTGCTGATCGGGTCGNGTANNNNNTannnnnn > 2:298894/1‑144 (MQ=255) cGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGAtgct < 1:1454/251‑1 (MQ=255) tCATTTGCTTAACCTTGGCCTCGCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTcg < 1:244737/115‑1 (MQ=255) aCCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCGACGTGCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCaaan < 1:61729/175‑2 (MQ=255) ctctCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgc < 1:9343/190‑1 (MQ=255) ctctCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGANNGGGTCGNNt > 2:9343/1‑99 (MQ=255) tctcCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGc < 1:206836/84‑1 (MQ=255) cTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGCCGATGGCGAAACTGCTGATCGGGTCGCGTAAAACCTACGGTCTGGATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTgcg > 1:168129/1‑177 (MQ=255) ccATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGt > 1:365641/1‑91 (MQ=255) aTTTCGTTGGTTTTGATACCTGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGTTCGCGTAAACCCTACGGTCTTAATCTGATTGGTGGTGGTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGGTGAAACGGGTGCGCGAGATGCGCGCCGACGTGTCggn < 1:55738/196‑2 (MQ=255) ttGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATgcg < 1:386587/175‑1 (MQ=255) cGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcg > 2:33032/1‑80 (MQ=255) cGGGAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTcg < 1:33032/80‑1 (MQ=255) gggAAAAGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCAc > 1:31421/1‑251 (MQ=255) aaaaGTCGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCAcccn < 1:298894/251‑2 (MQ=255) cGATGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCTCCCCTACGc < 1:120696/250‑1 (MQ=255) tGACGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCGGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTg < 1:425659/234‑1 (MQ=255) aCGATGGCGAAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATTGAACAACGCGCTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTa < 1:232325/251‑1 (MQ=255) | ATACCAATTCGGTTGAAAATGCACTGGGGATTGAGGTGCCGCAACGAGCACATACTATTCGCTCGATTCTGCTGGAAGTCGAACGGCTACACAGTCATTTGCTTAACCTTGGCCTCTCCTGCCATTTCGTTGGTTTTGATACCGGCTTTATGCAATTTTTCCGCGTGCGGGAAAAGTCGATGACGATGGCGGAATTGCTGATCGGGTCGCGTAAAACCTACGGTCTGAATCTGATTGGTGGTGTTCGCCGCGATATTCTCAAAGAGCAACGTCTGCAAACGCTGAAACTGGTGCGCGAGATGCGCGCCGACGTGTCGGAGCTGGTAGAGATGCTGCTTGCTACGCCGAATATGGAACAACGCACTCAGGGCATTGGCATTCTCGACCGACAAATCGCCCGTGATTTGCGCTTTGATCACCCCTACGCCGACTA > NC_000913/2609247‑2609679 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |