Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,286	G→A	A243V (GCT→GTT)	tsx ←	nucleoside‑specific channel‑forming protein Tsx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,286	0	G	A	100.0%	49.9 / NA	20	A243V (GCT→GTT)	tsx	nucleoside‑specific channel‑forming protein Tsx
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/8);  total (12/8)

GTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACG  >  NC_000913/431048‑431522                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             gTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATATggn                                                                                                                                                                                                                                  <  1:102405/251‑2 (MQ=255)                          aaGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCCTAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTACACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTAAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACg                                                                                                                                                                                                         >  1:235027/1‑251 (MQ=255)                           aGCACACGTTGGTAACAATCAGAAATGCCGGGAATAACTCCCGGCATTTTCATAATCAGAAGTTATAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATATGGCTGACAGCGATAGCGTTATTAGTACgg                                                                                                                                                                                                        >  1:259775/1‑251 (MQ=255)                                              cAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTGCAGTTCTGCATCGTCGTTCCACTGACCACCGGCGTGCCAGTAACGAGGTACGACAGAGTAGTGCCAGTGATCGTAGGTCAGAACCAGAGTAGGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTg                                                                                                                                                                                     <  1:279650/251‑1 (MQ=255)                                               aGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTgn                                                                                                                                                                                    <  1:406296/251‑2 (MQ=255)                                                                aTCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATATGGCGGGAAGCGTTAGAGGTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATccc                                                                                                                                                                   <  1:466134/251‑1 (MQ=255)                                                                                aaTCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACTAGAATATGGCTGGAAGCGATAGAGTTATTAGTACgg                                                                                                                                                                                                        <  1:184932/198‑1 (MQ=255)                                                                                                                 ccccAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTg                                                                                                                  >  1:162695/1‑251 (MQ=255)                                                                                                                               gCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACAGACCACCGTCGGGCCAGTAACGAGCTACGGCGAAGGAGTGTCAGTGATCGTAGTTGAGAACCAGAATATGGCTGGAAGCGATAGAGTGATTAGTACg                                                                                                                                                                                                         <  1:277271/150‑1 (MQ=255)                                                                                                                                                cgttgccgAAGTTCAGTTCTGNATCGTCGTTCCACTGACCACCGTCGTGCCATNANCGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATANGGCTGGAAGCNATANNGTTANTNGNACGGGACTTAATACCGTTGATTGCGTTACNGCTGTCATCCCCTAAATCGGAACNNCANNNGTNNTTggc                                                                                                                                       <  2:365822/199‑2 (MQ=255)                                                                                                                                                cgttgccgAAGTTCAGTTCTGCATCGTCGTTCCACTGCAAACCGTCGTGCAAGTAACGAGCTACGACAGAGTAGTGCCCGTGCTCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGAAAGGGTCTTAATACCGTTGATTGCGTTACCGTTGTCATCCCCTAAATCGGAACCCCAGCCGAAGTTggc                                                                                                                                       >  1:365822/1‑198 (MQ=255)                                                                                                                                                              agttcTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATCTGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATAACGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTACTTTTGa                                                                      >  1:201614/1‑250 (MQ=255)                                                                                                                                                                         gtcgtTCCACTGACCACCGTCGTGCCAGTAACGAGCTCCGACAGAGTAGTGCCCGGGATCGTGGTTCAGAACCCGACTATGGCTGGAAGCGATAGAGTTATTCGTACGGGGCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCt                                                                                                                                                                 >  1:224581/1‑148 (MQ=255)                                                                                                                                                                          tcgtTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTc                                                         >  1:436593/1‑251 (MQ=255)                                                                                                                                                                                                                 aCAGAGTAGTGACAGTGATCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGAc                                                                                                                >  1:164646/1‑157 (MQ=255)                                                                                                                                                                                                                           gCCAGTGATCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTg                                                                                                                                                                                         >  1:189251/1‑74 (MQ=255)                                                                                                                                                                                                                               gTGATCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTttcgttcg                                        >  1:119647/1‑215 (MQ=255)                                                                                                                                                                                                                                 naTCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGGTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATCGACg  >  1:406305/2‑251 (MQ=255)                                                                                                                                                                                                                                  aTCGTAGTTCAGAACCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCNNTAAATCNNa                                                                                                                                                         >  2:27808/1‑99 (MQ=255)                                                                                                                                                                                                                                   tngtngTTCANAACCNGAATATGGCTGGAAGNGATAGAGTGATTAGTACGGNNCNTAATGNCGTTg                                                                                                                                                                                         <  2:189251/66‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                             GTTTACGCCGCATCCGGCATGAACAAAGCACACGTTGTTAACAATCAGAAATGCCGGGAATAAATCCCGGCATTTTCATAATCAGAAGTTGTAACCTACTACCAGGTAACCACCCCAGCCGGTAGAGCGAACGTTGAAGTTGCCGTTGCCGAAGTTCAGTTCTGCATCGTCGTTCCACTGACCACCGTCGTGCCAGTAACGAGCTACGACAGAGTAGTGCCAGTGATCGTAGTTCAGAGCCAGAATATGGCTGGAAGCGATAGAGTTATTAGTACGGGTCTTAATACCGTTGATTGCGTTACCGCTGTCATCCCCTAAATCGGAACCCCAGTCGAAGTTGGTGAAGCCGATGTAGCTCAGCTGACCGCCCCACAGATCGGTAATCGGCACAAAGTATTTAATTTTGAAACGGTAACCGTCCCACTCGTTTTCGTTCGCTGCGCCATAGTTCTGCCACTGGTATTTCGCATAGACG  >  NC_000913/431048‑431522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑