Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	498,094	G→A	G14S (GGT→AGT)	hemH →	ferrochelatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	498,094	0	G	A	100.0%	51.6 / NA	19	G14S (GGT→AGT)	hemH	ferrochelatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/9);  total (10/9)

CAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCC  >  NC_000913/497881‑498339                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      cAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAAACTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACg                                                                                                                                                                                                                  >  1:263788/1‑251 (MQ=255)                                    gCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATGATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCGGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTgg                                                                                                                                                                               <  1:371029/251‑1 (MQ=255)                                      cGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTTGCAAACCTGAGTACGCCCTATGCCCCCACATCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGtt                                                                                                                                                                             <  1:458026/251‑1 (MQ=255)                                                            cAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTAACTTTTCCGCTACAATTATCACCAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCACCacac                                                                                                                                                                                                                                  >  1:275033/1‑176 (MQ=255)                                                              aaaaCCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTAtggtgg                                                                                                                                                     <  1:27027/251‑1 (MQ=255)                                                                             gcTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCGTCACTTTTCCGATCCAATTATCAACAAGTTGCATAGATCAGAGGCGGAAATGCGTTAGGCTAAAACAGGTATCCTGCTGGCAAAACTGAGTACGCACGACGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCgcggc                                                                                                                                      >  1:491297/1‑251 (MQ=255)                                                                                                   ccTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCACCACGTTGAATCGATAAGAGGCGGTAATGCGTCAGCCTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCTGACGCGGTAAAACGCTATCTGaaa                                                                                                                                                                                                        >  1:208040/1‑163 (MQ=255)                                                                                                        cacaGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGAGTCAGACTAAAACCGGTATCCTGCTGGCCAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTaa                                                                                                                                                                                                                      >  1:76890/1‑144 (MQ=255)                                                                                                                  aGTTCTTCTTCCTCACTTTTCCGCTACAAGTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCTACACCTGAAGCGGGAAAACGCTATCTGAAACAATTTTTAAGCTACAGACTCGTGTTTTATACCTCACGGTGGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGGTCGGCGCGTGTgg                                                                                                       <  1:417889/245‑1 (MQ=255)                                                                                                                                                tATCAACAAGTGGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACTCCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTTGTCATTGCTGCGCGGCGTGATTTTGCCGCTGCGTTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGc                                                                    <  1:65898/250‑1 (MQ=255)                                                                                                                                                      caaGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTcgccgn                                                             <  1:306847/251‑2 (MQ=255)                                                                                                                                                                cGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTg                                                                                                                                                                                                           <  1:397240/99‑1 (MQ=255)                                                                                                                                                                                           aaaaCCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGc                        <  1:80755/251‑1 (MQ=255)                                                                                                                                                                                              aCCGGTATCCTGCTGGCAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGAAGCGCGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCACCAGCAGGCGCTGGca                       >  1:442020/1‑249 (MQ=255)                                                                                                                                                                                                         gctgGCAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGCTGGttt                                                    >  1:241983/1‑209 (MQ=255)                                                                                                                                                                                                              cAAACCTGAGTACGCCCGATGCCCCCACACCTGNAGCGGNANANNGCNNTCTGAAAc                                                                                                                                                                                                       <  2:321906/57‑1 (MQ=255)                                                                                                                                                                                                              cAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGCTACCTCAAGGTTGTTATGATGGCCATTGCTGCGCGGCGTGATTTTGACGCTGCGCTcg                                                                                                                 >  1:263704/1‑143 (MQ=255)                                                                                                                                                                                                              cAAACCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGAAAAACGCTATCTGAAAc                                                                                                                                                                                                       >  1:321906/1‑57 (MQ=255)                                                                                                                                                                                                                 aCCTGAGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGTCGCGTGGTTGATACCTCACGGTTGTTATGGCGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGccc  >  1:246535/1‑251 (MQ=255)                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      CAGGCCCGCACAAGCAAACTTATCAGCAATCTCAGGCCGGATATTCATTCGGCCTTTTACAAAAACCTCAATCTGCGCTATTCCTTTTTCTGATTTGACCTCTCACAGCAATTAGTTCTTCTTCCTCACTTTTCCGCTACAATTATCAACAAGTTGAATCGATAAGAGGCGGTAATGCGTCAGACTAAAACCGGTATCCTGCTGGCAAACCTGGGTACGCCCGATGCCCCCACACCTGAAGCGGTAAAACGCTATCTGAAACAATTTTTAAGCGACAGACGCGTGGTTGATACCTCACGGTTGTTATGGTGGCCATTGCTGCGCGGCGTGATTTTGCCGCTGCGCTCGCCGCGTGTGGCGAAGCTGTATGCCTCTGTCTGGATGGAAGGTGGCTCGCCGCTGATGGTTTACAGCCGCCAGCAACAGCAGGCGCTGGCACAACGTTTACCGGAGATGCCC  >  NC_000913/497881‑498339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑