Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,065,028 C→T A76V (GCC→GTC)  scpC → propionyl‑CoA:succinate CoA transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,065,0280CT100.0% 58.0 / NA 22A76V (GCC→GTC) scpCpropionyl‑CoA:succinate CoA transferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/12);  total (10/12)

GAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGCCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAATTACGGTTTCTTCGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGC  >  NC_000913/3064790‑3065252
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                
aaagaataaaaaaTGAAAACTCAGTGGAAAAGTAGGCCCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGTTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCGAACGAACAGCATGAGGCCAAAAAGCCGTAGCAAATTCGCCTTCGGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTc                                                                                                                                                                                                                      <  1:70654/243‑1 (MQ=255)
           aaTGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGCTTGCCCGCCGAGCTAACGAACAGCATGAGGCCAAAACGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTgcgc                                                                                                                                                                                                           >  1:243231/1‑251 (MQ=255)
                              aGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCgn                                                                                                                                                                                        <  1:298539/251‑2 (MQ=255)
                                           aTGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTaaaa                                                                                                                                                                             >  1:175890/1‑249 (MQ=255)
                                                                                 gCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGAATGCACCCAGAGCTAACGCACAGCATGAGGACAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCCTATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCAttt                                                                                                                                     >  1:249475/1‑251 (MQ=255)
                                                                                                          gtgcGCCGAAATCCCTACCCACCTCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCTAAAAAGCCGTATAAAATTCGCCTGCTGACGGGGGCGTCAATCAGCGTCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTGTCCTGGCTTGCGCCATATCAAACATCGTCCGGGTTACGTAAAAAGATCAATCAGGGCGCGGTTAGTTTCGTTGACCTGCAtn                                                                                                                                      <  1:102454/222‑2 (MQ=255)
                                                                                                          gTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAAGTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTgn                                                                                                                                                                                                                  <  1:57674/149‑2 (MQ=255)
                                                                                                              gCCGAAAGCCCTACCCACCGCAATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGGCCGGTTGACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAATTACg                                                                                                        <  1:408977/251‑1 (MQ=255)
                                                                                                                cGAAAGCCCTACCCACCGCGAGTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGGGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACAtgg                                                                                                                                                                                             <  1:474952/164‑3 (MQ=255)
                                                                                                                           cccACCGCGATTGCCCGCAGAGCTAACGAACCGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTg                                                                                                                                                                                                                   >  1:153921/1‑131 (MQ=255)
                                                                                                                                                  tAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTAGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATAAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAg                                                                                                                                                       >  1:438689/1‑168 (MQ=255)
                                                                                                                                                           gCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGatca                                                                                                                                                                       >  1:220159/1‑143 (MQ=255)
                                                                                                                                                                    cAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAATTACGGTTTCTTCGGCGACATTGATGTTGCCGTCa                                                                          >  1:334992/1‑227 (MQ=255)
                                                                                                                                                                       naaGCCNTATNAAATTCGCCTTCTGACGGGTGCGGCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGGTNACGNAAAANGANCAATCAGGGCGCGGTNAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAANNANNNTNGCTTCGGc                                                                                             <  2:346272/204‑1 (MQ=255)
                                                                                                                                                                                                       cGTCAATCCGCGCCGCCGCTGACGATNTACTTTNNGANGTCGATGCTGTTTCCTGGCNTGNNNCNNNTNAAACANCGNNCGGTTTACGNAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTNGCGCAAATGNNCACTTACGGTTTCTTCGGCGANATTNNTNNTNCCGNCa                                                                          >  2:111826/1‑192 (MQ=255)
                                                                                                                                                                                                       cGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGGCGTCGATGCTGGGTCCGGGCGTGCGCCAGATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCg                                                                                                                                                  <  1:359542/120‑1 (MQ=255)
                                                                                                                                                                                                       cGTCAACCNGNNNCNNCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGNGCCATCTCAAACATCNTCNGGCTNACGNAACAAGATCAATCAGGGCGCGGTGAGTTTCg                                                                                                                                                  >  2:359542/1‑120 (MQ=255)
                                                                                                                                                                                                           aaTCAGCGCCGCCGCTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAATTACGGTTTCTGCGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATgcgc           <  1:111826/251‑1 (MQ=255)
                                                                                                                                                                                                               aGCGCCGCCTCTGACGATGTACTGTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGGAAAAAGAGCAATCAGGTCGCGGTGAGTTGCTTTGACCTGCATTTGAGCGAAGTGGCTCAAATGGGCAATTACGGTTTCTTCGTCGACAGTGATGTGGCCGTCATTGAAGCATCGGAACTGGCACCGGACGGTCGAGTCTGGTTAAcc                              <  1:356297/228‑1 (MQ=255)
                                                                                                                                                                                                                    ngccgcTGACGATGTACTTTCTGACGTCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTg                                                                                                                                                         >  1:132231/2‑100 (MQ=255)
                                                                                                                                                                                                                    cgccgcTGACGATGTACTTTCTGACGTCGATGCTTTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAATTACGGTTTCTTCGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGgc  <  1:29468/251‑1 (MQ=255)
                                                                                                                                                                                                                                        cNGANGTCGANGCTGNTTCCTGGCGTGCGCCNTATCAAACTTCGTCCGGTANNCNTAAAANGatca                                                                                                                                                                       <  2:220159/66‑1 (MQ=255)
                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                
GAAGGAGTAAAAATGGAAACTCAGTGGACAAGGATGACCGCCAATGAAGCGGCAGAAATTATCCAGCATAACGACATGGTGGCATTTAGCGGCTTTACCCCGGCGGGTTCGCCGAAAGCCCTACCCACCGCGATTGCCCGCAGAGCTAACGAACAGCATGAGGCCAAAAAGCCGTATCAAATTCGCCTTCTGACGGGTGCGTCAATCAGCGCCGCCGCTGACGATGTACTTTCTGACGCCGATGCTGTTTCCTGGCGTGCGCCATATCAAACATCGTCCGGTTTACGTAAAAAGATCAATCAGGGCGCGGTGAGTTTCGTTGACCTGCATTTGAGCGAAGTGGCGCAAATGGTCAATTACGGTTTCTTCGGCGACATTGATGTTGCCGTCATTGAAGCATCGGCACTGGCACCGGATGGTCGAGTCTGGTTAACCAGCGGGATCGGTAATGCGCCGACCTGGC  >  NC_000913/3064790‑3065252

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑