Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	622,820	G→A	G174D (GGC→GAC)	entS →	enterobactin exporter EntS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	622,820	0	G	A	100.0%	35.9 / NA	14	G174D (GGC→GAC)	entS	enterobactin exporter EntS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/9);  total (5/9)

TGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGG  >  NC_000913/622590‑623029                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  tGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCTGTGACGTAGCCTgn                                                                                                                                                                                                         <  1:132362/241‑2 (MQ=255)                            gnAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGCGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGGGGGGGGCACGGTTATTACCt                                                                                                                                                                    >  1:395590/1‑251 (MQ=255)                                 cGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTgg                                                                                                                                                                                                         <  1:64909/209‑1 (MQ=255)                                        gCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGc                                                                                                                                                                                               <  1:418538/212‑1 (MQ=255)                                                                                                            cggcgACACCAGCGCTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTACGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACTGGCTGGCGGCGGCGGGc                                                                                                                                                                                 <  1:31277/158‑1 (MQ=255)                                                                                                             ggcgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGCCCGTGCGTCTGGGGTCGGCGATTTCGCCCATGATTGGCGGTTTATTGATGGCGAACGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAg                                                                                                               >  1:257028/1‑223 (MQ=255)                                                                                                               cgACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAANTAc                                                                                                                                                                                                   >  2:305592/1‑137 (MQ=255)                                                                                                                               aGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGcc                                                                  <  1:219567/250‑1 (MQ=255)                                                                                                                                  gCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAAGCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGcc                                                              <  1:428784/251‑1 (MQ=255)                                                                                                                                   cGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGccn                                                             <  1:305592/251‑2 (MQ=255)                                                                                                                                                                  ccATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCtggt                                                       >  1:12423/1‑226 (MQ=255)                                                                                                                                                                                 gTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTAc                                                                                                                                                                                                   <  1:435304/71‑1 (MQ=255)                                                                                                                                                                                              gATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAATCATTACTGGCAGGATTTCGTGTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTTGGTGGTTTATTGACGATGGCGAGCGCGGTGCggg  <  1:427807/251‑1 (MQ=255)                                                                                                                                                                                                                              cGACCGGTGACGTAGCCTGGAACTACGGGCTGGCGGCGGCGCGCACGTTTATTCCCTTGCTACCGTTGTTACGCCTTCCGGCGTTGCCCCCGCCACCGCAGCCGCGTGCGTATCCGTTGCAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGggtg                                >  1:273502/1‑189 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                                  TGGACTGTGCCTTAATGCACTGCTGCCGGAGCCGTCATTGCTGGCAATCTATTTACTTGGTTTATGGGATGGTTTTTTCGCATCGCTTGGCGTTACGGCGCTATTGGCGGCGACACCAGCACTGGTAGGGCGTGAAAACTTAATGCAGGCCGGGGCGATCACCATGTTGACCGTGCGTCTGGGGTCGGTGATTTCGCCCATGATTGGCGGTTTATTGCTGGCGACCGGTGGCGTAGCCTGGAACTACGGGCTGGCGGCGGCGGGCACGTTTATTACCTTGCTACCGTTGTTAAGCCTTCCGGCGTTGCCACCGCCACCGCAGCCGCGTGAGCATCCGTTGAAATCATTACTGGCAGGATTTCGTTTTCTGCTTGCCAGCCCGCTGGTGGGCGGGATTGCGCTGCTGGGTGGTTTATTGACGATGGCGAGCGCGGTGCGGG  >  NC_000913/622590‑623029

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑