Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,713,679	C→T	H262Y (CAT→TAT)	srmB →	ATP‑dependent RNA helicase SrmB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,713,679	0	C	T	100.0%	48.6 / NA	17	H262Y (CAT→TAT)	srmB	ATP‑dependent RNA helicase SrmB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

GGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTAT  >  NC_000913/2713507‑2713891                                                                                                                                                                             |                                                                                                                                                                                                                     ggAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCTACCCTCTCAATTGTGTTGGTTCGTAAGCTTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCgg                                                                                                                                                                                        <  1:474472/203‑1 (MQ=255)                   ttCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTNGCTNGTGCNTCNGTTAAAACAGCCGGANGCGACCCGCTCAATTGAGTTTGTGCGTAAGCGTGAGCGTGTGTANNANNNGNCAAACTgg                                                                                                                                                                                                     <  2:356822/172‑1 (MQ=255)                     cTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCCTCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAAc                                                                                                                                      >  1:362030/1‑233 (MQ=255)                                                                  aTTACCGCGCCGATGATCTTGAGAATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCACCAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAAc                                                                                                                                      >  1:175813/1‑188 (MQ=255)                                                                     aCCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGc                                                                           <  1:163906/244‑1 (MQ=255)                                                                        gcgcCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGACGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGt                                                                 >  1:325139/1‑251 (MQ=255)                                                                                  tcgtGAGCAGAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCTGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATg                                                                                                                                                                                                                   <  1:331485/92‑1 (MQ=255)                                                                                                    gTTGCTGGAGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCCATTGTGTTTGTGAGTAAGCGAGAGAGTGAGTATGAGATGGCAAACAGGCTgca                                                                                                                                                                                                >  1:72519/1‑95 (MQ=255)                                                                                                          ggTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCggg                                                                                                                                                                                       >  1:399888/1‑99 (MQ=255)                                                                                                                 cTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTCTGCGCTGGCACACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGa                                                                                 >  1:281482/1‑194 (MQ=255)                                                                                                                   nttAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATcaaca                                                                                                                                                                               >  1:307359/2‑98 (MQ=255)                                                                                                                   gTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATcaaca                                                                                                                                                                               <  2:307359/98‑1 (MQ=255)                                                                                                                        aaCAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTgg                                                                                           <  1:173611/177‑1 (MQ=255)                                                                                                                          cAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCgcgc                 >  1:77732/1‑249 (MQ=255)                                                                                                                                       cccGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTAt  >  1:191383/1‑251 (MQ=255)                                                                                                                                                          gCGTANNCGNNNGCNNGAGTATGAGCTGGCAAACTGGCTNCGCGACGCGGGCATCAACAACTGCTATCTNGNANGTGANNTGGNACCGGACAAGNGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGNCNCAACCGATGTTGCCGCGCGCGGTATCGACATNCCTGACGTCAGCCACGTc                                      >  2:363769/1‑196 (MQ=255)                                                                                                                                                          gCGTAAGCGTGAGCGTGTGTATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGACAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCTCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTc                                      <  1:363769/196‑1 (MQ=255)                                                                                                                                                                             |                                                                                                                                                                                                                     GGAAGATCCGGTGGAAGTTTCTGCCAATCCCTCCACCCGTGAGCGCAAAAAAATTCATCAGTGGTATTACCGCGCCGATGATCTTGAGCATAAAACCGCGTTGCTGGTGCATCTGTTAAAACAGCCGGAAGCGACCCGCTCAATTGTGTTTGTGCGTAAGCGTGAGCGTGTGCATGAGCTGGCAAACTGGCTGCGCGAAGCGGGCATCAACAACTGCTATCTCGAAGGTGAGATGGTACAGGGCAAGCGTAACGAAGCGATCAAGCGTTTGACCGAAGGTCGCGTAAACGTACTGGTCGCAACCGATGTTGCCGCGCGCGGTATCGACATTCCTGACGTCAGCCACGTCTTTAACTTCGATATGCCGCGCAGTGGCGATACTTAT  >  NC_000913/2713507‑2713891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑