Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,698,650	G→A	intergenic (‑100/‑109)	pgpC ← / → yfhH	phosphatidylglycerophosphatase C/putative DNA‑binding transcriptional regulator YfhH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,698,650	0	G	A	100.0%	53.3 / NA	20	intergenic (‑100/‑109)	pgpC/yfhH	phosphatidylglycerophosphatase C/putative DNA‑binding transcriptional regulator YfhH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/11);  total (9/11)

CTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGG  >  NC_000913/2698406‑2698892                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   ntataATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCCCTTTTCCGGCAACAATCGACCGCATCATGACTAACt                                                                                                                                                                                                                                              >  1:297384/2‑251 (MQ=255)                                               ncGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCCATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTCATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTa                                                                                                                                                                                                                                    >  1:45986/2‑215 (MQ=255)                                                 gACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGc                                                                                                                                                                                                                           <  1:388442/222‑1 (MQ=255)                                                                          nccGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGACCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCtgtt                                                                                                                                                                     >  1:103911/2‑251 (MQ=255)                                                                                      ctgctgATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTAtt                                                                                                                                                         >  1:17147/1‑251 (MQ=255)                                                                                                                cAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACGGGAATATTTAAGTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGGGTGTCGCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGc                                                                                                                               <  1:286766/251‑1 (MQ=255)                                                                                                                       caccacACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTNGAANNNNNaannnnnn                                                                                                                                                                                                                             >  2:315180/1‑144 (MQ=255)                                                                                                                                  gCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCan                                                                                                             <  1:315180/251‑2 (MQ=255)                                                                                                                                                          nctctGGACTGAAACATCCTGATTACTTTTCGCTACTACATAGCCTAACAGATAGATCATCCCATTTCCGGCAACAATCGAGCGCATCCTGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCACTGGACTATCACATTCACTCGCGTGTGTCTCCTATTACCTGTTGCCGCCACTATTTAATCGCGAGGAAAGATATGACAGGCTTACTTCGTa                                                                                                                     >  1:60996/2‑219 (MQ=255)                                                                                                                                                                           ccTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCAGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGCATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCgg                                                                     >  1:330747/1‑250 (MQ=255)                                                                                                                                                                              gATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGTCTTACTTCGTATCCGTCAGCTTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGAt                                                                   <  1:475556/249‑1 (MQ=255)                                                                                                                                                                                        cGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATTACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATcn                                                                                                                  <  1:100083/192‑2 (MQ=255)                                                                                                                                                                                              tacATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCAGATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCg                                                                                                                       <  1:163841/181‑1 (MQ=255)                                                                                                                                                                                               acATAGCCTTACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCtn                                                <  1:149905/251‑2 (MQ=255)                                                                                                                                                                                                        anNAGATAGNNCATCACTTTTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCa                                                                                                                                                                                               <  2:37974/99‑1 (MQ=255)                                                                                                                                                                                                                 atnnnnNCTTNTCCGGCAACAATCGACCGCATCATGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGa                                                                                                                                         <  2:297384/144‑1 (MQ=255)                                                                                                                                                                                                                 ancatcACTTTTCCGGCAACAATCGACCGCATCATGANGAANNGGNNTTNTTAAt                                                                                                                                                                                                                                  <  2:286675/55‑1 (MQ=255)                                                                                                                                                                                                                                    caaTCGAACGCATCATGACTAACTGGACTTTTTAATTCATGGCAATTAGCGACAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCa            >  1:141319/1‑250 (MQ=255)                                                                                                                                                                                                                                               atcatGACTAACTGGAATTTTTAATTCATGGCAATTAGCCGCAATGGAATATACAATTCACTCCCGTGTGTCTAATATTGCCTGTTGTCGCCCCTATCTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACTAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCgg  >  1:504206/1‑249 (MQ=255)                                                                                                                                                                                                                                                 catGACTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGc              <  1:294337/235‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                   CTATAATCGGTAACAACGGCAGGACAAGTAACGCATTCAGCGGTTGGCGACGTAGTAAATAGCGCAGAAAACTGCCGAACATATCCTGCTGATGCAATGTTCCATCTAAGTCAAAAAACACCACACGACGCTCGTGAGTTGCCAAACCATACTCCTCTGGACTGAAACATCCTGATTAATTTTCGCTACTACATAGCCTAACAGATAGATCATCACTTTTCCGGCAACAATCGACCGCATCATGGCTAACTGGAATTTTTAATTCATGGCAATTAGCGGCAATGGAATATAAAATTCACTCGCGTGTGTCTCATATTGCCTGTTGTCGCCACTATTTCATCGCGAGGAAAGATATGAACGGCTTACTTCGTATCCGTCAGCGTTACCAGGGGCTTGCCCAGAGTGATAAAAAACTGGCGGATTATCTGCTGCTACAACCTGATACGGCGCGCCATTTAAGCTCTCAGCAACTGGCCAACGAAGCCGG  >  NC_000913/2698406‑2698892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑