| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,150,616 | C→T | L297L (CTG→TTG) | fabD → | [acyl‑carrier‑protein] S‑malonyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,150,616 | 0 | C | T | 90.0% | 19.1 / ‑3.3 | 10 | L297L (CTG→TTG) | fabD | [acyl‑carrier‑protein] S‑malonyltransferase |
| Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (5/4); total (6/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.49e-01 | |||||||||||
GCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTATTGGCAC > NC_000913/1150376‑1150773 | gCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGAAcc > 1:235062/1‑251 (MQ=255) gAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCAGCGTTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTg > 1:422129/1‑251 (MQ=255) ngAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGaa > 1:62302/2‑240 (MQ=255) cGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGc < 1:71893/251‑1 (MQ=255) gTCAGTTGTATAACCCGGTGCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGAAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCTGTGCAAGCCGCGGAATTGGc < 1:420867/251‑1 (MQ=255) ngcAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTgaacgaac > 1:91125/2‑102 (MQ=255) tctcTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGAACCTTCAGCGATGGCAgcn < 1:149468/103‑2 (MQ=255) ctATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTgg < 1:175926/155‑1 (MQ=255) ggCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGCACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCAc > 1:205772/1‑142 (MQ=255) nccGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGTTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTATTGGCAc > 1:308197/2‑219 (MQ=255) | GCGAAAATCACCTTTAACGCACCAACAGTTCCTGTTGTGAATAACGTTGATGTGAAATGCGAAACCAATGGTGATGCCATCCGTGACGCACTGGTACGTCAGTTGTATAACCCGGTTCAGTGGACGAAGTCTGTTGAGTACATGGCAGCGCAAGGCGTAGAACATCTCTATGAAGTCGGCCCGGGCAAAGTGCTTACTGGCCTGACGAAACGCATTGTCGACACCCTGACCGCCTCGGCGCTGAACGAACCTTCAGCGATGGCAGCGGCGCTCGAGCTTTAAAAGAGGAAAATCATGAATTTTGAAGGAAAAATCGCACTGGTAACCGGTGCAAGCCGCGGAATTGGCCGCGCAATTGCTGAAACGCTCGCAGCCCGTGGCGCGAAAGTTATTGGCAC > NC_000913/1150376‑1150773 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |