Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 869,418 G→A R3K (AGA→AAA)  gsiB → glutathione ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913869,4180GA100.0% 55.3 / NA 22R3K (AGA→AAA) gsiBglutathione ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

TACGCGTAAATTACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGC  >  NC_000913/869196‑869637
                                                                                                                                                                                                                              |                                                                                                                                                                                                                           
taCGCGTAAATTACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGgcg                                                                                                                                                                                                 >  1:247241/1‑251 (MQ=255)
                                  gAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAg                                                                                                                                                                                                              >  1:38753/1‑204 (MQ=255)
                                          ncGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCCTCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGCCAGCGTTg                                                                                                                                                                            >  1:43366/2‑230 (MQ=255)
                                                          gtagatgtgtgctcttccGATCT‑CCCAGCAATATTCATCTGCGTGGTGAAGAGGTGGCAGCCGTATCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGAAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTATTGGGTTAGTGGCGATGGGCATTGCGACAGCGTTTATGGCATCTTGTGCATTCGCTGCCAAAGATgtggtgg                                                                                                                                       <  1:342463/233‑1 (MQ=255)
                                                                                 tCCCATCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCTGGCCGGGGCATTACGGCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCATGCGGAGAATAAAAGGGCAAAAGCTTGACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCTCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTc                                                                                                                   <  1:484474/248‑1 (MQ=255)
                                                                                   ccAGCAATATTCATCTGCGTGGCGATGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGTACAACCACAATCAGAATACGCATTCATGCGAAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCAACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGGGGTGGGGGCGGAAGGATCGAAt                                                                                                                      >  1:374015/1‑243 (MQ=255)
                                                                                      gCNNNNNTCANCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTa                                                                                                                                                                                                                      <  2:299339/144‑1 (MQ=255)
                                                                                                                   gCAGCCGGCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACTCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAAGGGCAAAAGCTGTACACCGTAGTGGGGTAGAGGCGCTGGGCATGGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTcac                                                                                                                 <  1:360843/216‑1 (MQ=255)
                                                                                                                    cAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATAAGAATACGCATGCATGCGCAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGAAAACGATGTGGTGGTGGCGGTAGGAACGAATTTCACAACGCTCGATCCGTATGACGCAAATGACACGTTAtc                                                                             >  1:369705/1‑251 (MQ=255)
                                                                                                                       ccGTCTCGTTGTAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGACTACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGAAAAAGCTGTACACCGTAg                                                                                                                                                                                                              >  1:383884/1‑119 (MQ=255)
                                                                                                                                tGCAATGCGTCGGGCCGGGTCATTACGTCGCACAACCACAATCAGAATACTCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTacac                                                                                                                                                                                                                   <  1:248049/105‑1 (MQ=255)
                                                                                                                                           gggCCGGGGCATTACGTCGCACAACCACAATCAGAATCCGCATTCATGCCTAGATAACATTCAGGCGGAGAATAAAATGGCCAACGCTGTACACCGTAGTGGGTTCGTGACGCTGGGCATTGCGACAGCGTTCATGGCATCt                                                                                                                                                                   >  1:245634/1‑142 (MQ=255)
                                                                                                                                            ggCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAATATTCAGGCGGAGATTAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGAGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAGGc                                                                       >  1:467185/1‑233 (MQ=255)
                                                                                                                                                     aTTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTTCACCGTAGTGGGTTAGTGGCGCTGGGCATTTCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATTTGGTGGTGGTGGTAGGATCGAATTTCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAgg                                                                        <  1:493859/223‑1 (MQ=255)
                                                                                                                                                                   ncacaATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGAt                               >  1:127723/2‑250 (MQ=255)
                                                                                                                                                                    cacaATCAGAAGACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATGGCGACAGCGTTGATGGCAGCTGGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGa                                                                                                                            <  1:187716/156‑1 (MQ=255)
                                                                                                                                                                               tACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCtn                                                                                                                                                                                              <  1:307295/79‑2 (MQ=255)
                                                                                                                                                                               tACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTg                                                                                                                                                                                              >  2:307295/1‑79 (MQ=255)
                                                                                                                                                                                       cATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGAGGCGGTAGGATCGACTTTCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGaaacac          >  1:17037/1‑248 (MQ=255)
                                                                                                                                                                                                        cAGGCGGAGAATAAAATTGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATGGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGAGGTGGGTGTGGCGGGAGAATCGAATTGCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAGGCCTGAGCGAAATCGTTTTTCCAGGGGCGGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGc  <  1:75520/242‑1 (MQ=255)
                                                                                                                                                                                                                   tAAAATGGCAAAAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGGGGTGGCGGTAGGATCGAATTTCACCACGCTCGATCCGTATGACGCAAATTACACGTTATCTCAggn                                                                       <  1:405282/162‑2 (MQ=255)
                                                                                                                                                                                                                        tGGCANAAGCTNTNNACCGTAGTGGGTTAGTGGCNCTGGGCATTGCGACANCGTTNATGGcntcnt                                                                                                                                                                  >  2:210041/1‑66 (MQ=255)
                                                                                                                                                                                                                              |                                                                                                                                                                                                                           
TACGCGTAAATTACTGGCGGCAGTTCCGGTCGCTGAACCGTCCCGACAACGACCGCAGCGTGTACTGCTGTCGGACGATCTTCCCAGCAATATTCATCTGCGTGGCGAAGAGGTGGCAGCCGTCTCGTTGCAATGCGTCGGGCCGGGGCATTACGTCGCACAACCACAATCAGAATACGCATTCATGCGTAGATAACATTCAGGCGGAGAATAAAATGGCAAGAGCTGTACACCGTAGTGGGTTAGTGGCGCTGGGCATTGCGACAGCGTTGATGGCATCTTGTGCATTCGCTGCCAAAGATGTGGTGGTGGCGGTAGGATCGAATTTCACCACGCTCGATCCGTATGACGCAAATGACACGTTATCTCAGGCCGTAGCGAAATCGTTTTACCAGGGGCTGTTCGGTCTGGATAAAGAGATGAAACTGAAAAACGTGCTGGC  >  NC_000913/869196‑869637

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑