Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,396,373	C→T	T103T (ACC→ACT)	amiB →	N‑acetylmuramoyl‑L‑alanine amidase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,396,373	0	C	T	100.0%	40.3 / NA	16	T103T (ACC→ACT)	amiB	N‑acetylmuramoyl‑L‑alanine amidase B
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/8);  total (8/8)

GGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATC  >  NC_000913/4396151‑4396580                                                                                                                                                                                                                               |                                                                                                                                                                                                                ggTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGGGTTTTGTTGGCGATCCGGATTATGCGTTTAGCCATCAAAGAAAACTCACCGGGGCGCTCGATAACAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGGTAAGGCGATTCGCTCGGGAACGCCTAAAGATGCAGAAACGCTGCGGCTGGGGGTGGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGc                                                                                                                                                                                     <  1:271128/251‑1 (MQ=255)         aCGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGAAAACGCACCGTGGCGCTCGATATCAAACAACCGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGa                                                                                                                                                                               >  1:235767/1‑249 (MQ=255)                          nggATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGc                                                                                                                                                                                             >  1:147539/2‑218 (MQ=255)                                                     cnTGATTATTCGGTTAGCCATTAAAGCAAACGCNCNGGGTCGCTGGATATCAAACAAACGTGCGTGATTCAGGGACTGCCGTTGNGGGTCAGCGGNAATNNGCTGNTNANGGCGATTCGCGCTGGAACGCCTAAAGATGNACAAACGCTCCGGCTGGTGGTTGNNCTNNNTGNNAACgg                                                                                                                                                                                                         <  2:370642/179‑1 (MQ=255)                                                                        aTCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACgg                                                                                                                                                                                                         >  1:295834/1‑160 (MQ=255)                                                                                                 cGATATCAAACAAACGGGCATGATTCAGGGACTGCCGTTTTTGTTCTGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGTAACGCTTAAAGATGCACAAACTCTGCGGCTGGTGGTCGATCTTACTGAAAACGGTAAAGCCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACCCTGTCGTCTTTCCGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGa                                                                                       <  1:30109/249‑1 (MQ=255)                                                                                                  gagatCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCGACGGCTGGTGGGCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGGTGTGCCGCCATCGCCGGCTCCGCCGCCCGTCTTTGCGAAACGCTTTGAAAc                                                                                    <  1:419683/248‑1 (MQ=255)                                                                                                                     tGATTCAGGGCCTGCCGTTGTTGCTCAGAGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTCAGGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTCAAAACGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGACGTCTTTACGATTAACGCCGATGTGCCGCCACCGCATCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGCCGCACcg                                                                 >  1:426457/1‑251 (MQ=255)                                                                                                                                                                     ngATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGc                 >  1:318054/2‑251 (MQ=255)                                                                                                                                                                          cGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCctcctc                                                                                                                   >  1:140500/1‑148 (MQ=255)                                                                                                                                                                            ctctGGAACGCCTAAAGATGCACAAANGCTACGGCTNNTGNTCGAGNTNNNTNNNAANGGTAAAACCGAAGCGGTGANGCNNCAGAATNGCAGCAATTACACTGTCGTCTTTACg                                                                                                                                                  <  2:109239/115‑1 (MQ=255)                                                                                                                                                                            ctctGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGCAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACg                                                                                                                                                  >  1:109239/1‑115 (MQ=255)                                                                                                                                                                                ggAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACGTTCGACTGTACGATTAACGTCGATGTGCCGCCGCCGCCTCGTCCTCCGCTCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCTCACCGCGCGTCAGCGAACCTTCGCGCAATCCGTTTAAAACGTAAAGTAACCGCACTACggg         <  1:294876/248‑1 (MQ=255)                                                                                                                                                                                    cGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATc  <  1:77814/251‑1 (MQ=255)                                                                                                                                                                                         naaGATGCACAAACGCTACGGCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAAc                                                                                              >  1:303436/2‑154 (MQ=255)                                                                                                                                                                                             nnnnnnaanNNNNACGNCTGGTGGTCGATCTTACTGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAAGGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAAc                                                                                              <  2:303436/144‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                GGTTTCTAACGGTAATCAACAGGCGCGGATAACGTTGAGTTTTATTGGCGATCCTGATTATGCGTTTAGCCATCAAAGCAAACGCACCGTGGCGCTCGATATCAAACAAACGGGCGTGATTCAGGGACTGCCGTTGTTGTTCAGCGGCAATAATCTGGTGAAGGCGATTCGCTCTGGAACGCCTAAAGATGCACAAACGCTACGGCTGGTGGTCGATCTTACCGAAAACGGTAAAACCGAAGCGGTGAAGCGGCAGAATGGCAGCAATTACACTGTCGTCTTTACGATTAACGCCGATGTGCCGCCACCGCCTCCTCCGCCGCCCGTGGTTGCGAAACGCGTTGAAACGCCTGCGGTTGTCGCACCGCGCGTCAGCGAACCGGCGCGCAATCCGTTTAAAACGGAAAGTAACCGCACTACGGGTGTTATC  >  NC_000913/4396151‑4396580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑