Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,276,230	C→T	P463P (CCG→CCA)	narX ←	sensor histidine kinase NarX

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,276,230	0	C	T	92.9%	30.4 / ‑3.5	14	P463P (CCG→CCA)	narX	sensor histidine kinase NarX
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base T (8/5);  total (8/6)
Fisher's exact test for biased strand distribution p-value = 4.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.73e-01

GTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCA  >  NC_000913/1276054‑1276422                                                                                                                                                                                 |                                                                                                                                                                                                 gTCACCACGACTTCACTCGCTTGAGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCCATTAGCAACAAGGGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGAAATTGATAATCCAGCTTCCCCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGTTGCAtt                                                                                                                        >  1:402358/1‑251 (MQ=255)   caccacGACTTCACTCTCTTGCGAATGTTTGAGGGCGTTACTTAATTCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGACGGCAATTGATAATCCAGCTTCACCGCGAAGCCAAATTTTGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGTCGCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTc                                                                                                                       <  1:504941/250‑1 (MQ=255)                                                 gCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGACGGCACCAGGCGAGGCGGCAATTGCTCATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGc                                                                          >  1:270602/1‑249 (MQ=255)                                                           cAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATACTCCAGCTTCACCAAGAAGCCAAATTTGGCACTGTACTCTTCGCAAATCGCCTCCAGCGCTGGACGTACTACCGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACCGTTCGCGGCTGCTTTCTGGCAGCGCATCGccc                                                              >  1:345184/1‑251 (MQ=255)                                                                            aTTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATg                                                                                                                            <  1:202092/172‑1 (MQ=255)                                                                                          aGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCNATTNNNNNaann                                                                                                                                        >  2:312391/1‑144 (MQ=255)                                                                                          aGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAAGTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCan                                                                        <  1:312391/210‑2 (MQ=255)                                                                                               ccAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCa                                                                    <  1:26181/209‑1 (MQ=255)                                                                                                                 gATAATCCAGCTTCACCGGGAAGCCAAGTTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCAGTCGGTTCGTTAGGGATCTGACGTAACATTTCGCGGCTGCTTTCTGGCAGCGGATCGCCCTg                                                            <  1:453971/199‑1 (MQ=255)                                                                                                                              cACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGACTGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCt                                  >  1:117605/1‑212 (MQ=255)                                                                                                                              cACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCCCGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGa      >  1:381237/1‑240 (MQ=255)                                                                                                                               aCCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAAGGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGAc                                                                                                    <  1:222896/145‑1 (MQ=255)                                                                                                                                                 gcgcTGTACTCTTCGCAACTCGCCTACAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCa  >  1:398158/1‑225 (MQ=255)                                                                                                                                                         ctctTCGCAACTCGCCTCAAGCGCTGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCa                                                          >  1:136340/1‑161 (MQ=255)                                                                                                                                                                                 |                                                                                                                                                                                                 GTCACCACGACTTCACTCGCTTGCGAATGTTTGAGGGCGTTACTTAATGCCTCACGGGCAATTTGCAACAAGTGGATTGCCTGATGCGAAGGCACCAGGCGAGGCGGCAATTGATAATCCAGCTTCACCGGGAAGCCAAATTTGGCGCTGTACTCTTCGCAACTCGCCTCCAGCGCCGGACGTAATCCAGGCTCGGTGAGCTGCAAGCGGAATGTGGTGAGCAATTCACGCAACTGCGCCCAGGATGCATTCAGTTCGTTACGGATCTGACTTAACAGTTCGCGGCTGCTTTCTGGCAGCGCATCGCCCTGCATCTGTAAACAACTCACCTGCATCTTCATGCAAGAGAGAGATTGGGCAATAGAATCA  >  NC_000913/1276054‑1276422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑