Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,869,637	C→A	L87L (CTC→CTA)	cbrA →	colicin M resistance protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,869,637	0	C	A	100.0%	79.2 / NA	28	L87L (CTC→CTA)	cbrA	colicin M resistance protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/18);  total (10/18)

ATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGG  >  NC_000913/3869401‑3869862                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  attGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTAcc                                                                                                                                                                                                                      <  1:273842/250‑1 (MQ=255)                   ggATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCTGATCTCTAACGCGTAGCTACCAGCGAAGTGATATCAatat                                                                                                                                                                                                   <  1:175721/251‑1 (MQ=255)                    gATCGGCGTTGGCACGAAAGTTCGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGAACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTa                                                                                                                                                                                                                            >  1:209432/1‑225 (MQ=255)                                                cAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATg                                                                                                                                                                       >  1:340895/1‑250 (MQ=255)                                                                                              gAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGCCGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCtgtg                                                                                                                        >  1:218183/1‑251 (MQ=255)                                                                                                   cccgtccgattgatGCGGCGGGCTGCAGGCACCGGACGCGAAGCGATCTTTTATTCGCGATGGGCTTACGTTTCCTGTCGATGTGATCGCTAATCCGCAGAGATTCAGCTGCAAAACCAACGACGACGCTGCAACGCTAACGCGTAACTACCAGCGAAGCTATAAAAATATGAATCGCCATGCTTTCGACTGGTGGATGAAAACGCTGACCTCCGCCAGCGTTGTTGTTTATCACTACAGCCTGTGCCGaa                                                                                                                   <  1:494639/238‑1 (MQ=255)                                                                                                                               gCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGAc                                                                                                                                                                                <  1:214115/162‑1 (MQ=255)                                                                                                                                                  cTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCNNTAACGCNNa                                                                                                                                                                                                                            >  2:12005/1‑99 (MQ=255)                                                                                                                                                          cgcgATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAANCGTNGACGNCGNCGCATCGCTAACGCGNAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGANNTNNNGNTGAAATCg                                                                                                                                                                 <  2:345078/150‑1 (MQ=255)                                                                                                                                                          cgcgATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAACGTCAAAACCGTCGACGCCGCCGCATCGAAAACGCGTAACTCCCAGCGACGCTATATAAATCTTAATCGCCATGCTTTCGACTTGTGGATGAAATCg                                                                                                                                                                 >  1:345078/1‑150 (MQ=255)                                                                                                                                                                 ggCTGCCGCTTCCTGTCGATGTGATCTCTAATCCGCAGATTTTCAGCGTCAAAACCTCCGACGTCGCCGCATCGCTAACTCGTAACTACCAGCGAAGCTATATCAAAATTAATCGCCATGCTTTCGACTTGTTGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACat                                                     <  1:12005/251‑1 (MQ=255)                                                                                                                                                                            ccTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGACGCCGCCTCGCTAACGCGAAACTACCAGCCAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGCTGACATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGcc                                                                                                                      >  1:80810/1‑175 (MQ=255)                                                                                                                                                                              tGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGGCGCCGGATCGCTAACGCGTAACTGCCAGCGAAGCTATATGAATATTAACCGCCATGCTTTGGACTGGGGGAGGAAAACGCGGATCCCCGCCAGCGTTGATGTTTATCACGACAGCCTGTTCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAg                                                           <  1:279555/232‑1 (MQ=255)                                                                                                                                                                                  naNGTNATCGCNAATCNGCAGATTTTCAGCGTNAAAACCGTCGACGTCGCCGNNTNGCTAANGCGTa                                                                                                                                                                                                                            <  2:209432/66‑1 (MQ=255)                                                                                                                                                                                      tgATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCNAGCNNNNNgg                                                                                                                                           >  2:318131/1‑144 (MQ=255)                                                                                                                                                                                       gATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATTTCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTc                                <  1:288817/250‑1 (MQ=255)                                                                                                                                                                                          cGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCTCCAGCGTTGAGGTTTATCACTACAGCCTGTGCCGAAAAATc                                                                                                              <  1:164827/169‑1 (MQ=255)                                                                                                                                                                                            cTAATCCGCAGATTTTCAGCGTAAAACCCGTTGAGGTCGCCGCACCGCTATCGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGGGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTGTATCGCGACAGCCTGTGCCGAAAAATCGGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTg                                   <  1:207784/242‑1 (MQ=255)                                                                                                                                                                                                  cGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCATACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTcn                               <  1:53397/240‑2 (MQ=255)                                                                                                                                                                                                    cAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTGCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTAGCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATAGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGgcgc                  <  1:474623/251‑1 (MQ=255)                                                                                                                                                                                                           tCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTT‑CGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCg           <  1:450680/250‑1 (MQ=255)                                                                                                                                                                                                                gTCAAAACCGTCGACGTCGCCCCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATAAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCCAGACAGCCTGTGCCGAAAAATCTGGCGTTAGGAGGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAc                                                <  1:364270/209‑1 (MQ=255)                                                                                                                                                                                                                    aaaCCGTCGACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTgcgn  <  1:318131/251‑2 (MQ=255)                                                                                                                                                                                                                            gACGTCGCCGCATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGCCAGCCTGTGCCGAAAACTCTGGCGTGAGGCTGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTAccgcc                                            >  1:356894/1‑201 (MQ=255)                                                                                                                                                                                                                                 cgccgcATCGCTAACGCGTAACTACCAGCGAAGCTATATCACTATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATa                                                                                              >  1:450614/1‑146 (MQ=255)                                                                                                                                                                                                                                   ccgcATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGt                                                                                                        >  1:170486/1‑134 (MQ=255)                                                                                                                                                                                                                                     gcATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTgn                                                                                                           <  1:313374/129‑2 (MQ=255)                                                                                                                                                                                                                                     gcATCGCTAACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTGTAGCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTg                                                                         <  1:288154/163‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                  ATTGGCCTCGGCCCGGCAGGATCGGCGTTGGCACGAAAGTTAGCAGGCAAAATGCAGGTGATCGCGCTGGATAAAAAGCACCAGTGTGGTACTGAAGGTTTCAGCAAACCCTGCGGCGGTCTGCTGGCACCGGACGCGCAGCGATCTTTTATTCGCGATGGGCTGACGCTTCCTGTCGATGTGATCGCTAATCCGCAGATTTTCAGCGTCAAAACCGTCGACGTCGCCGCATCGCTCACGCGTAACTACCAGCGAAGCTATATCAATATTAATCGCCATGCTTTCGACTTGTGGATGAAATCGCTGATCCCCGCCAGCGTTGAGGTTTATCACGACAGCCTGTGCCGAAAAATCTGGCGTGAGGATGATAAATGGCATGTCATTTTTCGTGCAGACGGCTGGGAGCAACATATTACCGCCCGCTATCTGGTCGGTGCCGATGGCGCAAACTCGATGGTGCGG  >  NC_000913/3869401‑3869862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑