Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 516,955 C→T P125S (CCT→TCT)  fetB → putative iron ABC exporter membrane subunit FetB

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913516,9550CT100.0% 32.3 / NA 14P125S (CCT→TCT) fetBputative iron ABC exporter membrane subunit FetB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (4/10);  total (4/10)

ATTTTCAGCGTGGATGATGCCAGCCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTA‑TTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATG  >  NC_000913/516751‑517185
                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
atTTTCAGCGTGGATGATGCCAGCCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGTAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCTCTATCGCCGTGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGt                                                                                                                                                                                            <  1:177821/250‑1 (MQ=255)
                  nccAGCCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGGTTGAATTTATCCCCATGCAGGTGGTCTCTATCGCCGGGGTGATTGCCGGTAACGCCCTGGGAGCGGGGGGGTTGTGTTacaac                                                                                                                                                                                 >  1:123810/2‑243 (MQ=255)
                                                    gCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGGTTCTCTTAGGGTCGGTTGAATTTTTCCCGATTCAGGTGATCTCTATCGCCGGGGTGATTTCCGGGAAAGCCATGGTAGCGGTGGGGGtgtggta                                                                                                                                                                                     >  1:366651/1‑202 (MQ=255)
                                                    gCNTTAATGCGGCGTGGAACGCGCAAANACGCAGTAAATATATTGCTAAAGCTTTTATCNCNTCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGNTTCTCTCAGGNTCGNNTGAANTNANCCCGATGCAGGTGATCTCTATCGCCGGGANGATTGCCGGTAACGCCATGGTAGNNGTNNNGTNNTGTTa                                                                                                                                                                                     <  2:366651/205‑1 (MQ=255)
                                                                                                 cTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTTTCTCAGGGTCGATTGAATTTATTCCGATGCAGGGGATCTCTATCTCCGGGATGATTGGCGGGAACGCCATGGGAGCGGTGGGGTTGGGTTTCAACAATTTAGGGCAACGGGGCATTAGCGAAAAACAACCGATTCAGGAGAAACTGAGGTTTGGTGCGACGCCGAAGCAGGCTTCAGCGat                                                                                           >  1:38236/1‑250 (MQ=255)
                                                                                                                    gTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTGATCCCGATGCAGGTGATCTCTATCGCCGGGACGATTGCCTGTAACCCCATGGTAGCGGt                                                                                                                                                                                                 <  1:399973/129‑1 (MQ=255)
                                                                                                                    gTTTATTGCCAGTACGGGCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCTATGGAAGTGATCCCGATGCAGGTGATCTCTATCGCCGGTATGATTGCCGGTAACGCCATTGTAGCGGTGGGGTTGTGTTACAGCAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTTAGGAGAAACTg                                                                                                                              <  1:72757/196‑1 (MQ=255)
                                                                                                                                                           ggTGCTGATTCTCACAGAGACGATTGAATTTATCCCGATGCAGGTGATCTCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTGGTGTT‑CAACCATTTTGGGCAAAGGGGCATTAACGAAAAGCAACAGATTCAGGGGAAAATGGGGCTTGGTGGGGCGCCGAAGCAGGGCTCCGCGAAATTGATTAGGCACCGTAATTGGCG‑GGGTTTAATTCCGACGGGCGATTCCGCaaaaaaggg                              >  1:402402/1‑247 (MQ=255)
                                                                                                                                                                tgatgcgctcAGGGTCGATGGAATTTATCCCGATGCAGGTGAGATCTATCGCCGGGATGATTTCCGGTAACGCCAGGGTAGCGGTGGGGTTGTGTTacaac                                                                                                                                                                                 <  1:464310/94‑1 (MQ=255)
                                                                                                                                                                       ctcAGGGTCGATTGAATTTATCCCGATGCAGGTGATCTCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTA‑TTCGCGCGGCTTTAATGCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAg                      <  1:427127/248‑1 (MQ=255)
                                                                                                                                                                             gTCGATTGAATTTATCCCGATGCAGGTGATCTCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTA‑TTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGTCTTAGTGAGTTTAc             <  1:423320/251‑1 (MQ=255)
                                                                                                                                                                                  ttGAATTTATCCCGATGCAGGTGATCTCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTA‑TTCGCGCGGCTTTAATTcc                                                      <  1:169374/205‑1 (MQ=255)
                                                                                                                                                                                       nntANNNNNATGNAGGTGATCTCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGcc                                                                                                             <  2:302425/144‑1 (MQ=255)
                                                                                                                                                                                                    aGGTGATCTCTATCGCCGGGATGATTGCCGGTAACTCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTGGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTA‑TGCTCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAatgatg  <  1:495861/239‑1 (MQ=255)
                                                                                                                                                                                                            |                                                                                                                                                                                                                                       
ATTTTCAGCGTGGATGATGCCAGCCTGACATTATTGATGGTGTTATTTATCTGCTTTAATGCGGCGTGGAACGCGCAAAAACGCAGTAAATATATTGCTAAAGCTTTTATCTCATCGTTTATTGCCATTACGGTCGGGGCGGGAATTACCCTGGCGGTGCTGATTCTCTCAGGGTCGATTGAATTTATCCCGATGCAGGTGATCCCTATCGCCGGGATGATTGCCGGTAACGCCATGGTAGCGGTGGGGTTGTGTTACAACAATTTAGGGCAACGGGTCATTAGCGAACAGCAACAGATTCAGGAGAAACTGAGTCTTGGTGCGACGCCGAAGCAGGCTTCAGCGATATTGATTCGCGACAGTA‑TTCGCGCGGCTTTAATTCCGACGGTCGATTCAGCAAAAACGGTTGGCTTAGTGAGTTTACCAGGAATGATG  >  NC_000913/516751‑517185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑