| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 474,558 | C→T | A202T (GCT→ACT) | tesB ← | acyl‑CoA thioesterase II |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 474,558 | 0 | C | T | 93.8% | 36.3 / ‑6.1 | 16 | A202T (GCT→ACT) | tesB | acyl‑CoA thioesterase II |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (5/10); minor base G (1/0); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.75e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01 | |||||||||||
TGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGC > NC_000913/474359‑474799 | tGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGAAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGCACGCGCAGGTCATCCGGCACGCTACCAtt > 1:458715/1‑251 (MQ=255) nnnnnnnnncncgnnCACANAGCCACGTGCGCTGGACGCCGAGGNGCTCTCCACGCGATACAGCAGCCATTCATTCNANTTAAACGGGCGATTGAACCACATGGAATGGTCAATGGTGGCAATCTGANTCCCCGGTTCNAGANNACCGNTNCNGTGCGGCTGTAGAGCTACCGGCAGGAAGTNAAGAACAGAAGTGTAACCGAGCANNGANNNATNNAcgcg < 2:373655/211‑1 (MQ=255) gcACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGc < 1:29210/225‑1 (MQ=255) cacaAAGCCACGTGCGCTGGACGCCGCGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTCAACGGGCGATGGAACCACATGGCATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGCGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGAc > 1:220611/1‑251 (MQ=255) gcCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTgcg < 1:164968/185‑1 (MQ=255) caAATTAAGCGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTACGAGAAACCCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGGACGCTACCATTTGCGCGGATCCACACCGGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACcggacgg < 1:500143/244‑1 (MQ=255) tAAACNNGCNNNGGNNCAACATGGAATGGTCAATGGTGGNAATCTGAATCCCCGGTTCGAGAAAACCGANGNCNTGCGNNTGTNGCGCTACCGGNAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGCGGAACGCGCNGNTCATCAGGCCCGTTACCATTTGCGCGGGTCCNCACCTGACGATGTGGTTCTGCGAcncatcnnggnannnnnnnnnn > 2:377276/1‑202 (MQ=255) tAAACGGGCGATGGAACCACATTGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAATACCGATGCCGTGCGTCTGTAGAGCTACCGGCATGAAGTTAAGATCAGAAGTGTAACCGATCAGATACTGATTAACGCGCAGGGCATCCGGCACGCTACCATTTGCGCGGATCCACACCTTACGATGTGGTTCTGCGAc < 1:377276/202‑1 (MQ=255) ggCGATGGAACCACATGGAATGGTCAATAGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGAGGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCt < 1:497663/177‑1 (MQ=255) gCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCAGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCgn < 1:57949/251‑2 (MQ=255) cccGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGCTCAGAAGTGTCACCGAGCAGATACTGATGAACGCGCAGGTCATCCGACACCCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGAt > 1:79242/1‑212 (MQ=255) gCCGTGCGGCTGTAGAGCTACCGGCAGGAAGGTAAGATCAGAAGGGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGNGACNNNNNAg > 2:299177/1‑144 (MQ=255) ccGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGtttn < 1:299177/251‑2 (MQ=255) aaGATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGAGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAg > 1:167864/1‑157 (MQ=255) gATCAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTGCTGCGACGTGACCTGTCAGTGGGTTATGAAACTCCACCGGACGGACTGCCAGCGTACGACCGCAGATGAATGTATCTTTCAGCACGGGCGGCAGCAGGAGCGCCAGCGATGGGGCGATTTGCGTTTCCGAAGGGAGGCCATCTGGCGCTGGCGCGGACGGc < 1:453316/251‑1 (MQ=255) cAGAAGTGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATc < 1:235783/148‑1 (MQ=255) | TGGGTATAAAACTCACCGCGCACAAAGCCACGTGCGCTGGACGCCGAGGTGCTCTCCACGCTATACAGCAGCCATTCATTCAAATTAAACGGGCGATGGAACCACATGGAATGGTCAATGGTGGCAATCTGAATCCCCGGTTCGAGAAAACCGATGCCGTGCGGCTGTAGAGCTACCGGCAGGAAGTTAAGATCAGAAGCGTAACCGAGCAGATACTGATGAACGCGCAGGTCATCCGGCACGCTACCATTTGCGCGGATCCACACCTGACGATGTGGTTCTGCGACGTGACCTTTCAGTGGGTTATGAAACTCCACCGGACGGACTTCCAGCGGACGATCGCAGATGAATTTATCTTTCAGCACTGGCGGCAGCAGGTGCGCCAGCGATTGGGCGATTTGCGTTTCCGAAGGGAGGCCATCAGGCGCTGGCGCGGACGGC > NC_000913/474359‑474799 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |