Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,621,998	C→T	A77V (GCC→GTC)	yjjJ →	toxin YjjJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,621,998	0	C	T	100.0%	58.8 / NA	21	A77V (GCC→GTC)	yjjJ	toxin YjjJ
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/10);  total (11/10)

CGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGCCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGT  >  NC_000913/4621754‑4622236                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               cGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCACGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGAAGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACAt                                                                                                                                                                                                                                          >  1:334252/1‑251 (MQ=255)                                          gCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAgg                                                                                                                                                                                                                      <  1:408797/230‑1 (MQ=255)                                                 ccGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGCTATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGGTGTGCTGGCCGCAGAGAAGTTGTCTGGTAACa                                                                                                                                                                                                     >  1:257816/1‑240 (MQ=255)                                                                                  gCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAg                                                                                                                                                                                              >  1:262858/1‑214 (MQ=255)                                                                                   cAATCAGTCAGNCGACGTTCTCACGCCTTGTTGCCAGAGAAGANCNGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGNCCTTATCGCGNAATNNAGCGNANTNCCGTCTGGCGGGTGGACGATACCGGAAAGNCGCATAAATTCGTCGACATCCGGNNGTNNNGGNNGCAgg                                                                                                                                                                                                                      <  2:370815/189‑1 (MQ=255)                                                                                   cAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAgg                                                                                                                                                                                                                      >  1:370815/1‑189 (MQ=255)                                                                                                 aCGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATATCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCg                                                                                                                                          <  1:270991/251‑1 (MQ=255)                                                                                                                                gggTGATTCGCTTTGGTAAAGCACGGNCAACGCNNTANGCACTGCTGCGTCCTTATCNCGNNNTNNNGNGTATTNCCNNCTGGCGGGTNGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCCNGGAAGTTGTNNGGTAACAGGCGCAGATGGcgg                                                                                                                                                                                       >  2:113059/1‑174 (MQ=255)                                                                                                                                gggTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACGGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCTTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGcga                                                                                                                                                                                       <  1:113059/175‑1 (MQ=255)                                                                                                                                                  aaGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGttn                                                                                                                                                                                                                                   <  1:94765/113‑2 (MQ=255)                                                                                                                                                               gcgATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTggn                                                                                                                                                                                                                            <  1:47052/107‑2 (MQ=255)                                                                                                                                                                       naCTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGCAAGGCGCATACATTCGTCGACATCCGGTTGTGCTGGCCGCCGGGAAGTTGTCTGGTAACAGGCGCAGCTGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGgc                                                                                                                      >  1:55935/2‑201 (MQ=255)                                                                                                                                                                                                            cGTCTGGCGGGTGGCCGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGATGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCCGATGGCGACGAACAGAGGTTTGATGGTTTGCCCTGGTATTTGAc                                                                                                                                                   >  1:374986/1‑135 (MQ=255)                                                                                                                                                                                                            cGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCNTCGACATCCGNTTGNNCTTGNCNCNGGGAAGTTGTCTGGTAACAGGCGCAGATGNCGACGAACAGTGGTTTGATGGTTNNCCNNNGTNNTTGAc                                                                                                                                                   <  2:374986/135‑1 (MQ=255)                                                                                                                                                                                                                   cGGGTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTTGGGCAGGAAGTTATCCGCGCAACTGAAGCTGACTGATGATATACGTCTCGGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTAtt                        <  1:455336/251‑1 (MQ=255)                                                                                                                                                                                                                     ggTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGAc                              <  1:424778/243‑1 (MQ=255)                                                                                                                                                                                                                     ggTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCAGCAGGGAAGTTGTCTGGTAACAGGCGCCGATGGCGACGAACAGTGGTTTGATGGATTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTggg                                                                                                             >  1:294219/1‑164 (MQ=255)                                                                                                                                                                                                                      gTGGACGATACCGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTgg                                                                                                                                                           >  1:141245/1‑117 (MQ=255)                                                                                                                                                                                                                                ccGGANAGNNGCNNAAANTCATCGACATCCGGTTGTGCTGGCCGCAGGGAAGt                                                                                                                                                                                                                 >  2:276632/1‑53 (MQ=255)                                                                                                                                                                                                                                ccGGAAAGGCGCATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGGCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGGTTGCCGTGGTATTTGACCGATCTGCGACCGCAGGGCTTTTTAGGGCTCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATctgact                                                                             <  1:276632/185‑1 (MQ=255)                                                                                                                                                                                                                                         cgcATAAATTCGTCGACATCCGGTTGTGCTGGCCGCAGGGACGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGCTGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCggg  >  1:491802/1‑250 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                               CGAATCTATTCATTAATGAGCGAGCTGACTGATCTTTTACTGCAAGGGCCGCGTTCTGCCCCGGAATTGCGCCAGCGTCTGGCAATCAGTCAGGCGACGTTCTCACGCCTTGTTGCCAGAGAAGATCGGGTGATTCGCTTTGGTAAAGCACGGGCAACGCGATATGCACTGCTGCGTCCTTATCGCGGAATTGAGCGTATTCCCGTCTGGCGGGTGGACGATACCGGAAAGGCGCATAAATTCGCCGACATCCGGTTGTGCTGGCCGCAGGGAAGTTGTCTGGTAACAGGCGCAGATGGCGACGAACAGTGGTTTGATGGTTTGCCCTGGTATTTGACCGATCTCCGACCGCAGGGCTTTTTAGGGCGCGCGTGGGGCAGGAAGTTAGCCGCGCAACTGAATCTGACTGATGATATACGTCTCTGGCAGGAAGAAGATGTGCTCTACGCCCTGACCGTATTTAACGGTGAATATACTGGCGGT  >  NC_000913/4621754‑4622236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑