| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 35,476 | G→A | G229G (GGC→GGT) | caiD ← | crotonobetainyl‑CoA hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 35,476 | 0 | G | A | 92.9% | 34.6 / ‑7.2 | 14 | G229G (GGC→GGT) | caiD | crotonobetainyl‑CoA hydratase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (7/6); minor base T (0/1); total (7/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACG > NC_000913/35258‑35701 | gCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACACACGCCGTCGGGAGAACCAACGGAATTAACCACTCAAAGGCGTAATAGATCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTCATCGGCAAACGCCAGCGGCACTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGCGAat > 1:504623/1‑230 (MQ=255) gTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGa > 1:236678/1‑205 (MQ=255) aaCCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCGTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTcgcg < 1:346901/250‑1 (MQ=255) aaCGTCCTTTCCACACCAGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAg > 1:277683/1‑199 (MQ=255) cGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTGCGCTGGCGTTATCCAGCAGTTCCTCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTgcgc < 1:327208/251‑1 (MQ=255) cccTTCAATGGCATCTTCCGCATGAAGAACCGATGGCTAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAg > 1:413803/1‑186 (MQ=255) cccTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACTCCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTACGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTc < 1:496586/251‑1 (MQ=255) aTGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGct < 1:253253/69‑1 (MQ=255) cNAATGNAGNACCGATGTATAGTGTNTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCNNTNNNCNGGTGGTGc < 2:355653/86‑1 (MQ=255) cGAATGCAGAACCGATGGATAGTGTTTCAACACACCGCTGAGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGc > 1:355653/1‑86 (MQ=255) gAACCGATGGATAGTGTTTCAACTCACCTCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAg < 1:473588/94‑1 (MQ=255) ccGATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGtca > 1:113459/1‑231 (MQ=255) gATGGATAGTGTTTCAACACACCGCTGCGAATATAGCGATAc < 1:291460/42‑1 (MQ=255) gATAGTGTTTCAACACACCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGTCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCCTCACCATTTCATTGACg > 1:173594/1‑242 (MQ=255) | GCCGATGTAGACACCGGCTCCCACAATCACATCGCCAATCAAGACGGCACTGGGATGGACAAACGCCGTCGGGTGAACCACCGGAATTAACCCCTCAAAGGCGTAATAGCTCACGGTTGTTAACGTCCTTTCCACACCGGATCGCGCTTCTCGGCAAACGCCAGCGGCCCTTCAATGGCATCTTCCGAATGCAGAACCGATGGATAGTGTTTCAACACGCCGCTGCGAATATAGCGATACGCTTCTTCTACCGGCATTTCGCTGGTGGTGCGGTAGATCTCTTTCAGCGCCGCAATCGCCAGCGGGGCGCTGTTAACCAGCTGCTGAGCCAGTTCGCGGGCGTTATCCATCAGTTCCGCCTGGCTAACCACGCGGTTGACTATCCCCCAACGCAGCGCCTCTTCTGCGCCCATTCGTCTGCCGGTCATCACCATTTCATTGACG > NC_000913/35258‑35701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |